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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
261 L V -0.85 7.0 25
261 L I -1.10 7.0 25
261 L M -1.23 7.0 25
261 L F -0.60 7.0 25
261 L W -0.44 7.0 25
261 L Y -0.81 7.0 25
261 L G -5.01 7.0 25
261 L A -3.72 7.0 25
261 L P -1.49 7.0 25
261 L S -3.79 7.0 25
261 L T -3.67 7.0 25
261 L C -3.57 7.0 25
261 L H -1.37 7.0 25
261 L R -2.79 7.0 25
261 L K -2.95 7.0 25
261 L Q -2.12 7.0 25
261 L E -1.41 7.0 25
261 L N -2.93 7.0 25
261 L D -3.45 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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