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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
262 S V 1.16 7.0 25
262 S L 0.28 7.0 25
262 S I 0.76 7.0 25
262 S M -0.00 7.0 25
262 S F 0.74 7.0 25
262 S W 0.47 7.0 25
262 S Y -0.08 7.0 25
262 S G -1.77 7.0 25
262 S A -0.34 7.0 25
262 S P 0.02 7.0 25
262 S T -0.11 7.0 25
262 S C -1.71 7.0 25
262 S H -0.30 7.0 25
262 S R -0.70 7.0 25
262 S K 0.23 7.0 25
262 S Q -0.13 7.0 25
262 S E 0.52 7.0 25
262 S N 0.43 7.0 25
262 S D 0.61 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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