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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
263 Q V 0.66 7.0 25
263 Q L 0.41 7.0 25
263 Q I 0.53 7.0 25
263 Q M -1.28 7.0 25
263 Q F 0.28 7.0 25
263 Q W 0.04 7.0 25
263 Q Y 0.04 7.0 25
263 Q G -1.74 7.0 25
263 Q A -0.66 7.0 25
263 Q P -0.70 7.0 25
263 Q S -1.49 7.0 25
263 Q T -0.75 7.0 25
263 Q C -0.09 7.0 25
263 Q H -0.73 7.0 25
263 Q R -1.35 7.0 25
263 Q K -0.20 7.0 25
263 Q E 0.60 7.0 25
263 Q N -1.77 7.0 25
263 Q D -0.21 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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