I-Mutant2.0

Last Update 27/12/06



        
***************************************************************************
**                                                                       **
**                             I-Mutant v2.0                             **
**         Predictor of Protein Stability Changes upon Mutations         **
**                                                                       **
***************************************************************************


      SEQ File: fileseq.txt

      Position   WT  NEW     DDG    pH    T
            89    P    V    0.46   7.0   25
            89    P    L   -0.32   7.0   25
            89    P    I    0.20   7.0   25
            89    P    M   -1.15   7.0   25
            89    P    F   -0.61   7.0   25
            89    P    W   -0.76   7.0   25
            89    P    Y   -1.05   7.0   25
            89    P    G   -0.96   7.0   25
            89    P    A   -0.69   7.0   25
            89    P    S   -0.86   7.0   25
            89    P    T   -1.61   7.0   25
            89    P    C   -0.57   7.0   25
            89    P    H   -0.84   7.0   25
            89    P    R   -0.12   7.0   25
            89    P    K   -0.92   7.0   25
            89    P    Q   -1.04   7.0   25
            89    P    E   -0.04   7.0   25
            89    P    N   -1.37   7.0   25
            89    P    D   -0.77   7.0   25


      WT:  Aminoacid in Wild-Type Protein
      NEW: New Aminoacid after Mutation
      DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
             DDG<0: Decrease Stability
             DDG>0: Increase Stability
      T:   Temperature in Celsius degrees
      pH:  -log[H+]



***************************************************************************
*                                                                         *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting  *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310.                                         *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
*                                                                         *
***************************************************************************