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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
264 N V 1.26 7.0 25
264 N L 1.01 7.0 25
264 N I 1.46 7.0 25
264 N M 0.25 7.0 25
264 N F 0.45 7.0 25
264 N W 0.15 7.0 25
264 N Y -0.17 7.0 25
264 N G -2.68 7.0 25
264 N A -1.17 7.0 25
264 N P -0.01 7.0 25
264 N S -1.14 7.0 25
264 N T -0.40 7.0 25
264 N C -0.94 7.0 25
264 N H -1.49 7.0 25
264 N R -0.92 7.0 25
264 N K -0.89 7.0 25
264 N Q -1.03 7.0 25
264 N E 0.33 7.0 25
264 N D -0.49 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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