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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
245 R V 0.08 7.0 25
245 R L -0.74 7.0 25
245 R I 0.22 7.0 25
245 R M -1.06 7.0 25
245 R F -0.01 7.0 25
245 R W -0.21 7.0 25
245 R Y -0.24 7.0 25
245 R G -0.68 7.0 25
245 R A -0.81 7.0 25
245 R P -0.60 7.0 25
245 R S -2.81 7.0 25
245 R T -1.62 7.0 25
245 R C -0.92 7.0 25
245 R H -1.10 7.0 25
245 R K -0.80 7.0 25
245 R Q -0.78 7.0 25
245 R E -0.22 7.0 25
245 R N -1.14 7.0 25
245 R D -0.93 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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