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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
17 P V -0.85 5.0 60
17 P L -0.70 5.0 60
17 P I -0.22 5.0 60
17 P M -0.83 5.0 60
17 P F -0.25 5.0 60
17 P W -0.87 5.0 60
17 P Y -1.24 5.0 60
17 P G -1.56 5.0 60
17 P A -1.68 5.0 60
17 P S -1.62 5.0 60
17 P T -1.09 5.0 60
17 P C -1.12 5.0 60
17 P H -1.61 5.0 60
17 P R -1.01 5.0 60
17 P K -1.16 5.0 60
17 P Q -1.62 5.0 60
17 P E -0.73 5.0 60
17 P N -2.09 5.0 60
17 P D -1.80 5.0 60
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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