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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
266 T V 0.27 7.0 25
266 T L -0.53 7.0 25
266 T I -0.69 7.0 25
266 T M -0.37 7.0 25
266 T F 0.20 7.0 25
266 T W 0.04 7.0 25
266 T Y 0.89 7.0 25
266 T G -2.16 7.0 25
266 T A -1.26 7.0 25
266 T P -1.68 7.0 25
266 T S -0.57 7.0 25
266 T C -0.87 7.0 25
266 T H -1.45 7.0 25
266 T R -0.44 7.0 25
266 T K -1.01 7.0 25
266 T Q -1.44 7.0 25
266 T E 0.18 7.0 25
266 T N -0.41 7.0 25
266 T D 0.39 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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