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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
269 R V 0.25 7.0 25
269 R L 0.08 7.0 25
269 R I 0.21 7.0 25
269 R M -0.69 7.0 25
269 R F 0.02 7.0 25
269 R W -0.17 7.0 25
269 R Y -0.05 7.0 25
269 R G -1.18 7.0 25
269 R A -0.45 7.0 25
269 R P -0.56 7.0 25
269 R S -2.19 7.0 25
269 R T -0.70 7.0 25
269 R C -0.72 7.0 25
269 R H -0.33 7.0 25
269 R K -1.18 7.0 25
269 R Q -0.70 7.0 25
269 R E 0.03 7.0 25
269 R N -0.99 7.0 25
269 R D -0.58 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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