***************************************************************************
** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
***************************************************************************
SEQ File: fileseq.txt
Position WT NEW DDG pH T
123 S V 0.45 7.5 50
123 S L 0.07 7.5 50
123 S I 0.20 7.5 50
123 S M -0.16 7.5 50
123 S F 0.09 7.5 50
123 S W 0.06 7.5 50
123 S Y -0.31 7.5 50
123 S G -0.87 7.5 50
123 S A -0.16 7.5 50
123 S P -1.66 7.5 50
123 S T -0.48 7.5 50
123 S C -0.34 7.5 50
123 S H 0.20 7.5 50
123 S R 0.17 7.5 50
123 S K -0.04 7.5 50
123 S Q -0.45 7.5 50
123 S E -0.11 7.5 50
123 S N -0.49 7.5 50
123 S D -0.14 7.5 50
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
***************************************************************************
* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
***************************************************************************