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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
92 A V 0.81 7.0 25
92 A L 0.53 7.0 25
92 A I 0.56 7.0 25
92 A M 0.34 7.0 25
92 A F 0.21 7.0 25
92 A W -0.72 7.0 25
92 A Y -0.89 7.0 25
92 A G -1.25 7.0 25
92 A P -1.17 7.0 25
92 A S -0.57 7.0 25
92 A T -1.23 7.0 25
92 A C -0.02 7.0 25
92 A H -1.40 7.0 25
92 A R -0.06 7.0 25
92 A K -1.02 7.0 25
92 A Q -0.60 7.0 25
92 A E -0.95 7.0 25
92 A N -0.83 7.0 25
92 A D -1.26 7.0 25
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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