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** **
** I-Mutant v2.0 **
** Predictor of Protein Stability Changes upon Mutations **
** **
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SEQ File: fileseq.txt
Position WT NEW DDG pH T
311 Q V 0.70 7.5 50
311 Q L 0.52 7.5 50
311 Q I 0.61 7.5 50
311 Q M 0.00 7.5 50
311 Q F 1.20 7.5 50
311 Q W 0.60 7.5 50
311 Q Y 0.91 7.5 50
311 Q G -0.21 7.5 50
311 Q A -0.02 7.5 50
311 Q P -1.00 7.5 50
311 Q S -0.47 7.5 50
311 Q T -0.01 7.5 50
311 Q C 1.06 7.5 50
311 Q H 0.51 7.5 50
311 Q R 0.28 7.5 50
311 Q K -0.06 7.5 50
311 Q E 0.50 7.5 50
311 Q N -1.25 7.5 50
311 Q D 0.24 7.5 50
WT: Aminoacid in Wild-Type Protein
NEW: New Aminoacid after Mutation
DDG: DG(NewProtein)-DG(WildType) in Kcal/mol
DDG<0: Decrease Stability
DDG>0: Increase Stability
T: Temperature in Celsius degrees
pH: -log[H+]
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* *
* Capriotti E, Fariselli P and Casadio R (2005). I-Mutant2.0: predicting *
* stability changes upon mutation from the protein sequence or structure. *
* Nucl. Acids Res. 33: W306-W310. *
* http://gpcr.biocomp.unibo.it/cgi/predictors/I-Mutant2.0/I-Mutant2.0.cgi *
* *
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