[ { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "WT(L112A,Y132W)", "MUTATION_PDB": "WT(L22A,Y42W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 12.25, "ln(ku)_H2O": 6.35, "dGKIN_H2O": 14.64, "mf": -2.93, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "BACKGROUND_MUT_PDB": [ "WT" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.081", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "WT(L112A,Y132W)", "MUTATION_PDB": "WT(L22A,Y42W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 12.25, "ln(ku)_H2O": 6.35, "dGKIN_H2O": 14.64, "mf": -2.93, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "BACKGROUND_MUT_PDB": [ "WT" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.081", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A139G", "MUTATION_PDB": "A139G", "SEC_STR": "Helix", "RSA": "0.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.97, "dln(kf)_H2O": -1.26, "ln(ku)_H2O": 6.8, "dln(ku)_H2O": 3.8, "mf": -2.01, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.022", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A139G", "MUTATION_PDB": "A139G", "SEC_STR": "Helix", "RSA": "0.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.97, "dln(kf)_H2O": -1.26, "ln(ku)_H2O": 6.8, "dln(ku)_H2O": 3.8, "mf": -2.01, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.022", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E141A", "MUTATION_PDB": "E141A", "SEC_STR": "Helix", "RSA": "70.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -0.19, "ln(ku)_H2O": 4.17, "dln(ku)_H2O": 1.17, "mf": -2.34, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.023", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E141A", "MUTATION_PDB": "E141A", "SEC_STR": "Helix", "RSA": "70.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -0.19, "ln(ku)_H2O": 4.17, "dln(ku)_H2O": 1.17, "mf": -2.34, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.023", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V144A", "MUTATION_PDB": "V144A", "SEC_STR": "Coil", "RSA": "4.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.73, "dln(kf)_H2O": -1.5, "ln(ku)_H2O": 5.48, "dln(ku)_H2O": 2.48, "mf": -2.47, "mu": 0.88, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.024", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V144A", "MUTATION_PDB": "V144A", "SEC_STR": "Coil", "RSA": "4.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.73, "dln(kf)_H2O": -1.5, "ln(ku)_H2O": 5.48, "dln(ku)_H2O": 2.48, "mf": -2.47, "mu": 0.88, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.024", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D145N", "MUTATION_PDB": "D145N", "SEC_STR": "Coil", "RSA": "42.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.55, "dln(kf)_H2O": -0.68, "ln(ku)_H2O": 4.09, "dln(ku)_H2O": 1.09, "mf": -2.09, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.025", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D145N", "MUTATION_PDB": "D145N", "SEC_STR": "Coil", "RSA": "42.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.55, "dln(kf)_H2O": -0.68, "ln(ku)_H2O": 4.09, "dln(ku)_H2O": 1.09, "mf": -2.09, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.025", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D145A", "MUTATION_PDB": "D145A", "SEC_STR": "Coil", "RSA": "42.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.81, "dln(kf)_H2O": -1.42, "ln(ku)_H2O": 4.7, "dln(ku)_H2O": 1.7, "mf": -2.05, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.026", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D145A", "MUTATION_PDB": "D145A", "SEC_STR": "Coil", "RSA": "42.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.81, "dln(kf)_H2O": -1.42, "ln(ku)_H2O": 4.7, "dln(ku)_H2O": 1.7, "mf": -2.05, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.026", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I146V", "MUTATION_PDB": "I146V", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.2, "dln(kf)_H2O": -1.03, "ln(ku)_H2O": 5.7, "dln(ku)_H2O": 2.7, "mf": -1.92, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.027", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I146V", "MUTATION_PDB": "I146V", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.2, "dln(kf)_H2O": -1.03, "ln(ku)_H2O": 5.7, "dln(ku)_H2O": 2.7, "mf": -1.92, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.027", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L159A", "MUTATION_PDB": "L159A", "SEC_STR": "Coil", "RSA": "35.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.56, "dln(kf)_H2O": -1.67, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": 2.44, "mf": -2.68, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.030", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L159A", "MUTATION_PDB": "L159A", "SEC_STR": "Coil", "RSA": "35.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.56, "dln(kf)_H2O": -1.67, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": 2.44, "mf": -2.68, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.030", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D162N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "17.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.07, "dln(kf)_H2O": -2.16, "ln(ku)_H2O": 4.55, "dln(ku)_H2O": 1.55, "mf": -2.8, "mu": 0.96, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.031", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D162N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "17.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.07, "dln(kf)_H2O": -2.16, "ln(ku)_H2O": 4.55, "dln(ku)_H2O": 1.55, "mf": -2.8, "mu": 0.96, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.031", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I163V", "MUTATION_PDB": "I163V", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -0.69, "ln(ku)_H2O": 3.91, "dln(ku)_H2O": 0.91, "mf": -2.34, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.032", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I163V", "MUTATION_PDB": "I163V", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -0.69, "ln(ku)_H2O": 3.91, "dln(ku)_H2O": 0.91, "mf": -2.34, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.032", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D164N", "MUTATION_PDB": "D164N", "SEC_STR": "Helix", "RSA": "52.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.24, "dln(kf)_H2O": -0.99, "ln(ku)_H2O": 4.87, "dln(ku)_H2O": 1.87, "mf": -2.09, "mu": 0.96, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.033", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D164N", "MUTATION_PDB": "D164N", "SEC_STR": "Helix", "RSA": "52.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.24, "dln(kf)_H2O": -0.99, "ln(ku)_H2O": 4.87, "dln(ku)_H2O": 1.87, "mf": -2.09, "mu": 0.96, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.033", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D164A", "MUTATION_PDB": "D164A", "SEC_STR": "Helix", "RSA": "52.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.81, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 6.43, "dln(ku)_H2O": 3.43, "mf": -2.3, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.034", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D164A", "MUTATION_PDB": "D164A", "SEC_STR": "Helix", "RSA": "52.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.81, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 6.43, "dln(ku)_H2O": 3.43, "mf": -2.3, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.034", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A165G", "MUTATION_PDB": "A165G", "SEC_STR": "Helix", "RSA": "48.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.9, "dln(kf)_H2O": -1.33, "ln(ku)_H2O": 4.09, "dln(ku)_H2O": 1.09, "mf": -2.43, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.035", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A165G", "MUTATION_PDB": "A165G", "SEC_STR": "Helix", "RSA": "48.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.9, "dln(kf)_H2O": -1.33, "ln(ku)_H2O": 4.09, "dln(ku)_H2O": 1.09, "mf": -2.43, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.035", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V149A", "MUTATION_PDB": "V149A", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.37, "dln(kf)_H2O": -1.86, "ln(ku)_H2O": 5.48, "dln(ku)_H2O": 2.48, "mf": -2.47, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.028", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V149A", "MUTATION_PDB": "V149A", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.37, "dln(kf)_H2O": -1.86, "ln(ku)_H2O": 5.48, "dln(ku)_H2O": 2.48, "mf": -2.47, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.028", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V158A", "MUTATION_PDB": "V158A", "SEC_STR": "Beta", "RSA": "0.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 7.94, "dln(kf)_H2O": -2.29, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 3.86, "mf": -3.93, "mu": 0.38, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.029", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V158A", "MUTATION_PDB": "V158A", "SEC_STR": "Beta", "RSA": "0.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 7.94, "dln(kf)_H2O": -2.29, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 3.86, "mf": -3.93, "mu": 0.38, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.029", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L167A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "48.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.99, "dln(kf)_H2O": -1.24, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 1.38, "mf": -2.51, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.036", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L167A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "48.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.99, "dln(kf)_H2O": -1.24, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 1.38, "mf": -2.51, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.036", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "WT(F166W)", "MUTATION_PDB": "WT", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.23, "ln(ku)_H2O": 3.0, "mf": -2.3, "mu": 1.26, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.016", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "WT(F166W)", "MUTATION_PDB": "WT", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.23, "ln(ku)_H2O": 3.0, "mf": -2.3, "mu": 1.26, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.016", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V129A", "MUTATION_PDB": "V129A", "SEC_STR": "Coil", "RSA": "24.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": -0.16, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 2.35, "mf": -2.18, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.016", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V129A", "MUTATION_PDB": "V129A", "SEC_STR": "Coil", "RSA": "24.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": -0.16, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 2.35, "mf": -2.18, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.016", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I130A", "MUTATION_PDB": "I130A", "SEC_STR": "Coil", "RSA": "37.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.74, "dln(kf)_H2O": -0.49, "ln(ku)_H2O": 5.99, "dln(ku)_H2O": 2.99, "mf": -2.01, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.017", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I130A", "MUTATION_PDB": "I130A", "SEC_STR": "Coil", "RSA": "37.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.74, "dln(kf)_H2O": -0.49, "ln(ku)_H2O": 5.99, "dln(ku)_H2O": 2.99, "mf": -2.01, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.017", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I130G", "MUTATION_PDB": "I130G", "SEC_STR": "Coil", "RSA": "37.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.78, "dln(kf)_H2O": -0.45, "ln(ku)_H2O": 6.21, "dln(ku)_H2O": 3.21, "mf": -1.92, "mu": 0.59, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.018", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I130G", "MUTATION_PDB": "I130G", "SEC_STR": "Coil", "RSA": "37.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.78, "dln(kf)_H2O": -0.45, "ln(ku)_H2O": 6.21, "dln(ku)_H2O": 3.21, "mf": -1.92, "mu": 0.59, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.018", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "UPF0337 protein YjbJ", "SOURCE": "Escherichia coli", "LENGTH": 69.0, "UniProt": "P68206", "PDB_wild": "1ryk", "MUTATED_CHAIN": "A", "PFAM": [ "PF05532" ], "CATH": [ "1rykA00 (1.10.1470.10)" ], "MUTATION_PDB": "WT", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "NaPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.08, "ln(ku)_H2O": 4.49, "dGKIN_H2O": 11.37, "AUTHOR_KIN": "Maxwell, K.L., Wildes, D., Zarrine-Afsar, A., De Los Rios, M.A., Brown, A.G., Friel, C.T., Hedberg, L., Horng, J.-C., Bona, D., Miller, E.J., Vall\u00e9e-B\u00e9lisle, A., Main, E.R.G., Bemporad, F., Qiu, L., Teilum, K., Vu, N.-D., Edwards, A.M., Ruczinski, I., Poulsen, F.M., Kragelund, B.B., Michnick, S.W., Chiti, F., Bai, Y., Hagen, S.J., Serrano, L., Oliveberg, M., Raleigh, D.P., Wittung-Stafshede, P., Radford, S.E., Jackson, S.E., Sosnick, T.R., Marqusee, S., Davidson, A.R., Plaxco, K.W.", "REFERENCE_KIN": "Protein folding: defining a \u201cstandard\u201d set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci. 14, 602\u2013616. https://doi.org/10.1110/ps.041205428", "YEAR_KIN": 2005.0, "PMID_KIN": "15689503", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "UPF0337 protein YjbJ", "SOURCE": "Escherichia coli", "LENGTH": 69.0, "UniProt": "P68206", "PDB_wild": "1ryk", "MUTATED_CHAIN": "A", "PFAM": [ "PF05532" ], "CATH": [ "1rykA00 (1.10.1470.10)" ], "MUTATION_PDB": "WT", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "NaPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.08, "ln(ku)_H2O": 4.49, "dGKIN_H2O": 11.37, "AUTHOR_KIN": "Maxwell, K.L., Wildes, D., Zarrine-Afsar, A., De Los Rios, M.A., Brown, A.G., Friel, C.T., Hedberg, L., Horng, J.-C., Bona, D., Miller, E.J., Vall\u00e9e-B\u00e9lisle, A., Main, E.R.G., Bemporad, F., Qiu, L., Teilum, K., Vu, N.-D., Edwards, A.M., Ruczinski, I., Poulsen, F.M., Kragelund, B.B., Michnick, S.W., Chiti, F., Bai, Y., Hagen, S.J., Serrano, L., Oliveberg, M., Raleigh, D.P., Wittung-Stafshede, P., Radford, S.E., Jackson, S.E., Sosnick, T.R., Marqusee, S., Davidson, A.R., Plaxco, K.W.", "REFERENCE_KIN": "Protein folding: defining a \u201cstandard\u201d set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci. 14, 602\u2013616. https://doi.org/10.1110/ps.041205428", "YEAR_KIN": 2005.0, "PMID_KIN": "15689503", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A131G", "MUTATION_PDB": "A131G", "SEC_STR": "Beta", "RSA": "14.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.79, "dln(kf)_H2O": -0.44, "ln(ku)_H2O": 6.82, "dln(ku)_H2O": 3.82, "mf": -2.05, "mu": 0.42, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.019", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A131G", "MUTATION_PDB": "A131G", "SEC_STR": "Beta", "RSA": "14.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.79, "dln(kf)_H2O": -0.44, "ln(ku)_H2O": 6.82, "dln(ku)_H2O": 3.82, "mf": -2.05, "mu": 0.42, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.019", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V135A", "MUTATION_PDB": "V135A", "SEC_STR": "Helix", "RSA": "2.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.93, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 6.51, "dln(ku)_H2O": 3.51, "mf": -1.92, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.020", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V135A", "MUTATION_PDB": "V135A", "SEC_STR": "Helix", "RSA": "2.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.93, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 6.51, "dln(ku)_H2O": 3.51, "mf": -1.92, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.020", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "Y138F", "MUTATION_PDB": "Y138F", "SEC_STR": "Helix", "RSA": "18.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.45, "dln(kf)_H2O": -0.78, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 3.15, "mf": -1.92, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.021", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "E3BD (F166W)", "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)", "LENGTH": 45.0, "UniProt": "P11961", "PDB_wild": "1w4e", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4eA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "Y138F", "MUTATION_PDB": "Y138F", "SEC_STR": "Helix", "RSA": "18.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump or stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.45, "dln(kf)_H2O": -0.78, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 3.15, "mf": -1.92, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "F166W" ], "AUTHOR_KIN": "Ferguson, N., Sharpe, T.D., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.021", "YEAR_KIN": 2006.0, "PMID_KIN": "16406408", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FF domain from HYPA/FBP11", "SOURCE": "Homo sapiens", "LENGTH": 70.0, "UniProt": "O75400", "PDB_wild": "1uzc", "MUTATED_CHAIN": "A", "PFAM": [ "PF01846" ], "CATH": [ "1uzcA00 (1.10.10.440)" ], "MUTATION_UNIPROT": "L431A", "MUTATION_PDB": "L52A", "SEC_STR": "Coil", "RSA": "0.6", "T": 10.0, "pH": 5.7, "BUFFER_NAME": "Na-Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "heat", "ln(kf)_H2O": 6.02, "dln(kf)_H2O": -1.68, "ln(ku)_H2O": 5.94, "dln(ku)_H2O": 4.64, "dGKIN_H2O": 3.14, "ddGKIN_H2O": -12.09, "mf": -4.52, "mu": -0.63, "AUTHOR_KIN": "Jemth P, Day R, Gianni S, Khan F, Allen M, Daggett V, Fersht AR", "REFERENCE_KIN": "The structure of the major transition state for folding of an FF domain from experiment and simulation. J Mol Biol. 350(2):363-78. https://doi.org/10.1016/j.jmb.2005.04.067", "YEAR_KIN": 2005.0, "PMID_KIN": "15935381", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "FF domain from HYPA/FBP11", "SOURCE": "Homo sapiens", "LENGTH": 70.0, "UniProt": "O75400", "PDB_wild": "1uzc", "MUTATED_CHAIN": "A", "PFAM": [ "PF01846" ], "CATH": [ "1uzcA00 (1.10.10.440)" ], "MUTATION_UNIPROT": "L431A", "MUTATION_PDB": "L52A", "SEC_STR": "Coil", "RSA": "0.6", "T": 10.0, "pH": 5.7, "BUFFER_NAME": "Na-Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "heat", "ln(kf)_H2O": 6.02, "dln(kf)_H2O": -1.68, "ln(ku)_H2O": 5.94, "dln(ku)_H2O": 4.64, "dGKIN_H2O": 3.14, "ddGKIN_H2O": -12.09, "mf": -4.52, "mu": -0.63, "AUTHOR_KIN": "Jemth P, Day R, Gianni S, Khan F, Allen M, Daggett V, Fersht AR", "REFERENCE_KIN": "The structure of the major transition state for folding of an FF domain from experiment and simulation. J Mol Biol. 350(2):363-78. https://doi.org/10.1016/j.jmb.2005.04.067", "YEAR_KIN": 2005.0, "PMID_KIN": "15935381", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "WT(H125W)", "MUTATION_PDB": "WT(H142W)", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 11.17, "ln(ku)_H2O": 6.65, "dGKIN_H2O": 10.67, "mf": -2.34, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "WT(H125W)", "MUTATION_PDB": "WT(H142W)", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 11.17, "ln(ku)_H2O": 6.65, "dGKIN_H2O": 10.67, "mf": -2.34, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L114A", "MUTATION_PDB": "L131A", "SEC_STR": "Coil", "RSA": "6.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.34, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 7.32, "dln(ku)_H2O": 0.67, "dGKIN_H2O": 7.11, "ddGKIN_H2O": -3.56, "mf": -2.09, "mu": 0.75, "PhiF_H2O": 0.52, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L114A", "MUTATION_PDB": "L131A", "SEC_STR": "Coil", "RSA": "6.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.34, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 7.32, "dln(ku)_H2O": 0.67, "dGKIN_H2O": 7.11, "ddGKIN_H2O": -3.56, "mf": -2.09, "mu": 0.75, "PhiF_H2O": 0.52, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "S115G", "MUTATION_PDB": "S132G", "SEC_STR": "Coil", "RSA": "34.6", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.61, "dln(kf)_H2O": -0.56, "ln(ku)_H2O": 6.49, "dln(ku)_H2O": -0.16, "dGKIN_H2O": 9.67, "ddGKIN_H2O": -1.0, "mf": -2.09, "mu": 0.88, "PhiF_H2O": 0.55, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "S115G", "MUTATION_PDB": "S132G", "SEC_STR": "Coil", "RSA": "34.6", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.61, "dln(kf)_H2O": -0.56, "ln(ku)_H2O": 6.49, "dln(ku)_H2O": -0.16, "dGKIN_H2O": 9.67, "ddGKIN_H2O": -1.0, "mf": -2.09, "mu": 0.88, "PhiF_H2O": 0.55, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "I118A", "MUTATION_PDB": "I135A", "SEC_STR": "Helix", "RSA": "4.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.47, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 8.37, "dln(ku)_H2O": 1.72, "dGKIN_H2O": 4.94, "ddGKIN_H2O": -5.73, "mf": -1.92, "mu": 0.84, "PhiF_H2O": 0.32, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "I118A", "MUTATION_PDB": "I135A", "SEC_STR": "Helix", "RSA": "4.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.47, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 8.37, "dln(ku)_H2O": 1.72, "dGKIN_H2O": 4.94, "ddGKIN_H2O": -5.73, "mf": -1.92, "mu": 0.84, "PhiF_H2O": 0.32, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L121A", "MUTATION_PDB": "L138A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.18, "dln(kf)_H2O": -0.99, "ln(ku)_H2O": 8.58, "dln(ku)_H2O": 1.93, "dGKIN_H2O": 3.77, "ddGKIN_H2O": -6.9, "mf": -1.92, "mu": 0.67, "PhiF_H2O": 0.35, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L121A", "MUTATION_PDB": "L138A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.18, "dln(kf)_H2O": -0.99, "ln(ku)_H2O": 8.58, "dln(ku)_H2O": 1.93, "dGKIN_H2O": 3.77, "ddGKIN_H2O": -6.9, "mf": -1.92, "mu": 0.67, "PhiF_H2O": 0.35, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A123G", "MUTATION_PDB": "A140G", "SEC_STR": "Helix", "RSA": "52.8", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.36, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 7.14, "dln(ku)_H2O": 0.49, "dGKIN_H2O": 7.57, "ddGKIN_H2O": -3.1, "mf": -1.76, "mu": 0.96, "PhiF_H2O": 0.49, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A123G", "MUTATION_PDB": "A140G", "SEC_STR": "Helix", "RSA": "52.8", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.36, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 7.14, "dln(ku)_H2O": 0.49, "dGKIN_H2O": 7.57, "ddGKIN_H2O": -3.1, "mf": -1.76, "mu": 0.96, "PhiF_H2O": 0.49, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L127A", "MUTATION_PDB": "L144A", "SEC_STR": "Coil", "RSA": "24.4", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 8.29, "dln(ku)_H2O": 1.64, "dGKIN_H2O": 5.02, "ddGKIN_H2O": -5.65, "mf": -1.72, "mu": 0.75, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L127A", "MUTATION_PDB": "L144A", "SEC_STR": "Coil", "RSA": "24.4", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 8.29, "dln(ku)_H2O": 1.64, "dGKIN_H2O": 5.02, "ddGKIN_H2O": -5.65, "mf": -1.72, "mu": 0.75, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A129G", "MUTATION_PDB": "A146G", "SEC_STR": "Helix", "RSA": "18.9", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.75, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 6.81, "dln(ku)_H2O": 0.16, "dGKIN_H2O": 9.29, "ddGKIN_H2O": -1.38, "mf": -2.09, "mu": 0.84, "PhiF_H2O": 0.42, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A129G", "MUTATION_PDB": "A146G", "SEC_STR": "Helix", "RSA": "18.9", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.75, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 6.81, "dln(ku)_H2O": 0.16, "dGKIN_H2O": 9.29, "ddGKIN_H2O": -1.38, "mf": -2.09, "mu": 0.84, "PhiF_H2O": 0.42, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A131G", "MUTATION_PDB": "A148G", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 6.36, "dln(ku)_H2O": -0.29, "dGKIN_H2O": 10.21, "ddGKIN_H2O": -0.46, "mf": -1.8, "mu": 1.17, "PhiF_H2O": 0.52, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "A131G", "MUTATION_PDB": "A148G", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 6.36, "dln(ku)_H2O": -0.29, "dGKIN_H2O": 10.21, "ddGKIN_H2O": -0.46, "mf": -1.8, "mu": 1.17, "PhiF_H2O": 0.52, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "I132V", "MUTATION_PDB": "I149V", "SEC_STR": "Coil", "RSA": "8.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.44, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": -0.17, "dGKIN_H2O": 9.37, "ddGKIN_H2O": -1.3, "mf": -1.97, "mu": 0.92, "PhiF_H2O": 0.59, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "I132V", "MUTATION_PDB": "I149V", "SEC_STR": "Coil", "RSA": "8.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.44, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": -0.17, "dGKIN_H2O": 9.37, "ddGKIN_H2O": -1.3, "mf": -1.97, "mu": 0.92, "PhiF_H2O": 0.59, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "T135S", "MUTATION_PDB": "T152S", "SEC_STR": "Coil", "RSA": "73.9", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.02, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 8.63, "dln(ku)_H2O": 1.98, "dGKIN_H2O": 3.26, "ddGKIN_H2O": -7.41, "mf": -2.01, "mu": 0.63, "PhiF_H2O": 0.39, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "T135S", "MUTATION_PDB": "T152S", "SEC_STR": "Coil", "RSA": "73.9", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.02, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 8.63, "dln(ku)_H2O": 1.98, "dGKIN_H2O": 3.26, "ddGKIN_H2O": -7.41, "mf": -2.01, "mu": 0.63, "PhiF_H2O": 0.39, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "V137G", "MUTATION_PDB": "V154G", "SEC_STR": "Coil", "RSA": "80.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.24, "dln(kf)_H2O": -0.93, "ln(ku)_H2O": 6.63, "dln(ku)_H2O": -0.02, "dGKIN_H2O": 8.49, "ddGKIN_H2O": -2.18, "mf": -1.97, "mu": 1.0, "PhiF_H2O": 0.54, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "V137G", "MUTATION_PDB": "V154G", "SEC_STR": "Coil", "RSA": "80.3", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.24, "dln(kf)_H2O": -0.93, "ln(ku)_H2O": 6.63, "dln(ku)_H2O": -0.02, "dGKIN_H2O": 8.49, "ddGKIN_H2O": -2.18, "mf": -1.97, "mu": 1.0, "PhiF_H2O": 0.54, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L141A", "MUTATION_PDB": "L158A", "SEC_STR": "Coil", "RSA": "3.0", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -1.63, "ln(ku)_H2O": 8.73, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 1.92, "ddGKIN_H2O": -8.75, "mf": -2.01, "mu": 0.96, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L141A", "MUTATION_PDB": "L158A", "SEC_STR": "Coil", "RSA": "3.0", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -1.63, "ln(ku)_H2O": 8.73, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 1.92, "ddGKIN_H2O": -8.75, "mf": -2.01, "mu": 0.96, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "T142S", "MUTATION_PDB": "T159S", "SEC_STR": "Coil", "RSA": "23.2", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.25, "dln(kf)_H2O": -0.92, "ln(ku)_H2O": 7.68, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 6.07, "ddGKIN_H2O": -4.6, "mf": -2.22, "mu": 0.84, "PhiF_H2O": 0.39, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "T142S", "MUTATION_PDB": "T159S", "SEC_STR": "Coil", "RSA": "23.2", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.25, "dln(kf)_H2O": -0.92, "ln(ku)_H2O": 7.68, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 6.07, "ddGKIN_H2O": -4.6, "mf": -2.22, "mu": 0.84, "PhiF_H2O": 0.39, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "D145N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "18.4", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.48, "dln(kf)_H2O": -1.69, "ln(ku)_H2O": 8.09, "dln(ku)_H2O": 1.44, "dGKIN_H2O": 3.26, "ddGKIN_H2O": -7.41, "mf": -2.01, "mu": 0.71, "PhiF_H2O": 0.48, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "D145N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "18.4", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.48, "dln(kf)_H2O": -1.69, "ln(ku)_H2O": 8.09, "dln(ku)_H2O": 1.44, "dGKIN_H2O": 3.26, "ddGKIN_H2O": -7.41, "mf": -2.01, "mu": 0.71, "PhiF_H2O": 0.48, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "V146A", "MUTATION_PDB": "V163A", "SEC_STR": "Helix", "RSA": "2.1", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.9, "dln(kf)_H2O": -1.27, "ln(ku)_H2O": 9.06, "dln(ku)_H2O": 2.41, "dGKIN_H2O": 1.97, "ddGKIN_H2O": -8.7, "mf": -2.01, "mu": 0.67, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "V146A", "MUTATION_PDB": "V163A", "SEC_STR": "Helix", "RSA": "2.1", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.9, "dln(kf)_H2O": -1.27, "ln(ku)_H2O": 9.06, "dln(ku)_H2O": 2.41, "dGKIN_H2O": 1.97, "ddGKIN_H2O": -8.7, "mf": -2.01, "mu": 0.67, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "H149G", "MUTATION_PDB": "H166G", "SEC_STR": "Helix", "RSA": "24.5", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.35, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 8.71, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 3.89, "ddGKIN_H2O": -6.78, "mf": -1.88, "mu": 0.88, "PhiF_H2O": 0.25, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "H149G", "MUTATION_PDB": "H166G", "SEC_STR": "Helix", "RSA": "24.5", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.35, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 8.71, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 3.89, "ddGKIN_H2O": -6.78, "mf": -1.88, "mu": 0.88, "PhiF_H2O": 0.25, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L150A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "28.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.42, "dln(kf)_H2O": -0.75, "ln(ku)_H2O": 6.65, "dGKIN_H2O": 8.91, "ddGKIN_H2O": -1.76, "mf": -2.26, "mu": 0.67, "PhiF_H2O": 0.71, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (H142W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L150A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "28.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.42, "dln(kf)_H2O": -0.75, "ln(ku)_H2O": 6.65, "dGKIN_H2O": 8.91, "ddGKIN_H2O": -1.76, "mf": -2.26, "mu": 0.67, "PhiF_H2O": 0.71, "BACKGROUND_MUT_UNIPROT": [ "H125W" ], "BACKGROUND_MUT_PDB": [ "H142W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (L131W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "WT(L114W)", "MUTATION_PDB": "WT(L131W)", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.4, "ln(ku)_H2O": 6.46, "dGKIN_H2O": 9.29, "mf": -2.18, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "L114W" ], "BACKGROUND_MUT_PDB": [ "L131W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (L131W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "WT(L114W)", "MUTATION_PDB": "WT(L131W)", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 10.4, "ln(ku)_H2O": 6.46, "dGKIN_H2O": 9.29, "mf": -2.18, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "L114W" ], "BACKGROUND_MUT_PDB": [ "L131W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (L131W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L150A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "28.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.6, "dln(kf)_H2O": -0.8, "ln(ku)_H2O": 7.0, "dln(ku)_H2O": 0.54, "dGKIN_H2O": 6.11, "ddGKIN_H2O": -3.18, "mf": -2.3, "mu": 0.59, "PhiF_H2O": 0.61, "BACKGROUND_MUT_UNIPROT": [ "L114W" ], "BACKGROUND_MUT_PDB": [ "L131W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BBL domain (L131W)", "SOURCE": "Escherichia coli", "LENGTH": 45.0, "UniProt": "P0AFG6", "PDB_wild": "2wxc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "2wxcA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.61", "MUTATION_UNIPROT": "L150A", "MUTATION_PDB": "L167A", "SEC_STR": "Helix", "RSA": "28.7", "T": 10.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 9.6, "dln(kf)_H2O": -0.8, "ln(ku)_H2O": 7.0, "dln(ku)_H2O": 0.54, "dGKIN_H2O": 6.11, "ddGKIN_H2O": -3.18, "mf": -2.3, "mu": 0.59, "PhiF_H2O": 0.61, "BACKGROUND_MUT_UNIPROT": [ "L114W" ], "BACKGROUND_MUT_PDB": [ "L131W" ], "AUTHOR_KIN": "Neuweiler H, Sharpe TD, Rutherford TJ, Johnson CM, Allen MD, Ferguson N, Fersht AR", "REFERENCE_KIN": "The folding mechanism of BBL: Plasticity of transition-state structure observed within an ultrafast folding protein family. J Mol Biol.390(5):1060-73. https://doi.org/10.1016/j.jmb.2009.05.011", "YEAR_KIN": 2009.0, "PMID_KIN": "19445954", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "WT(L30W)", "MUTATION_PDB": "WT(L31W)", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.06, "ln(ku)_H2O": 2.08, "dGKIN_H2O": 14.07, "mf": -1.76, "mu": 0.88, "CM_KIN": -2.64, "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "WT(L30W)", "MUTATION_PDB": "WT(L31W)", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.06, "ln(ku)_H2O": 2.08, "dGKIN_H2O": 14.07, "mf": -1.76, "mu": 0.88, "CM_KIN": -2.64, "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Y4A", "MUTATION_PDB": "Y5A", "SEC_STR": "Coil", "RSA": "8.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Y4A", "MUTATION_PDB": "Y5A", "SEC_STR": "Coil", "RSA": "8.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Y4F", "MUTATION_PDB": "Y5F", "SEC_STR": "Coil", "RSA": "8.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.82, "dln(kf)_H2O": -0.24, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 3.27, "dGKIN_H2O": 5.81, "ddGKIN_H2O": -8.26, "mf": -1.67, "mu": 0.92, "CM_KIN": -2.59, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Y4F", "MUTATION_PDB": "Y5F", "SEC_STR": "Coil", "RSA": "8.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.82, "dln(kf)_H2O": -0.24, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 3.27, "dGKIN_H2O": 5.81, "ddGKIN_H2O": -8.26, "mf": -1.67, "mu": 0.92, "CM_KIN": -2.59, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S5A", "MUTATION_PDB": "S6A", "SEC_STR": "Coil", "RSA": "61.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S5A", "MUTATION_PDB": "S6A", "SEC_STR": "Coil", "RSA": "61.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S5G", "MUTATION_PDB": "S6G", "SEC_STR": "Coil", "RSA": "61.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S5G", "MUTATION_PDB": "S6G", "SEC_STR": "Coil", "RSA": "61.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L7A", "MUTATION_PDB": "L8A", "SEC_STR": "Coil", "RSA": "37.2", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.21, "dln(kf)_H2O": -1.85, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 4.61, "ddGKIN_H2O": -9.46, "mf": -2.18, "mu": 0.67, "CM_KIN": -2.85, "PhiF_H2O": 0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L7A", "MUTATION_PDB": "L8A", "SEC_STR": "Coil", "RSA": "37.2", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.21, "dln(kf)_H2O": -1.85, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 4.61, "ddGKIN_H2O": -9.46, "mf": -2.18, "mu": 0.67, "CM_KIN": -2.85, "PhiF_H2O": 0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "T8S", "MUTATION_PDB": "T9S", "SEC_STR": "Coil", "RSA": "27.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "T8S", "MUTATION_PDB": "T9S", "SEC_STR": "Coil", "RSA": "27.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "T8A", "MUTATION_PDB": "T9A", "SEC_STR": "Coil", "RSA": "27.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.4, "dln(kf)_H2O": -1.66, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 8.0, "ddGKIN_H2O": -6.07, "mf": -1.84, "mu": 1.17, "CM_KIN": -3.01, "PhiF_H2O": 0.6, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "T8A", "MUTATION_PDB": "T9A", "SEC_STR": "Coil", "RSA": "27.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.4, "dln(kf)_H2O": -1.66, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 8.0, "ddGKIN_H2O": -6.07, "mf": -1.84, "mu": 1.17, "CM_KIN": -3.01, "PhiF_H2O": 0.6, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A", "SEC_STR": "Helix", "RSA": "45.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A", "SEC_STR": "Helix", "RSA": "45.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9G", "MUTATION_PDB": "V10G", "SEC_STR": "Helix", "RSA": "45.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.58, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 2.08, "dGKIN_H2O": 12.94, "ddGKIN_H2O": -1.13, "mf": -1.76, "mu": 1.13, "CM_KIN": -2.89, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9G", "MUTATION_PDB": "V10G", "SEC_STR": "Helix", "RSA": "45.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.58, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 2.08, "dGKIN_H2O": 12.94, "ddGKIN_H2O": -1.13, "mf": -1.76, "mu": 1.13, "CM_KIN": -2.89, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A,V11A", "SEC_STR": "Helix,Helix", "RSA": "45.8,61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A,V11A", "SEC_STR": "Helix,Helix", "RSA": "45.8,61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V10A", "MUTATION_PDB": "V11A", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V10A", "MUTATION_PDB": "V11A", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V10G", "MUTATION_PDB": "V11G", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V10G", "MUTATION_PDB": "V11G", "SEC_STR": "Helix", "RSA": "61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q11A", "MUTATION_PDB": "Q12A", "SEC_STR": "Helix", "RSA": "55.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.04, "dln(kf)_H2O": -0.02, "ln(ku)_H2O": 4.61, "dln(ku)_H2O": 2.53, "dGKIN_H2O": 8.07, "ddGKIN_H2O": -6.0, "mf": -2.97, "mu": 0.08, "CM_KIN": -3.05, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q11A", "MUTATION_PDB": "Q12A", "SEC_STR": "Helix", "RSA": "55.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.04, "dln(kf)_H2O": -0.02, "ln(ku)_H2O": 4.61, "dln(ku)_H2O": 2.53, "dGKIN_H2O": 8.07, "ddGKIN_H2O": -6.0, "mf": -2.97, "mu": 0.08, "CM_KIN": -3.05, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q11G", "MUTATION_PDB": "Q12G", "SEC_STR": "Helix", "RSA": "55.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.94, "dln(kf)_H2O": -1.12, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 6.33, "ddGKIN_H2O": -7.74, "mf": -2.13, "mu": 0.75, "CM_KIN": -2.89, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q11G", "MUTATION_PDB": "Q12G", "SEC_STR": "Helix", "RSA": "55.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.94, "dln(kf)_H2O": -1.12, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 6.33, "ddGKIN_H2O": -7.74, "mf": -2.13, "mu": 0.75, "CM_KIN": -2.89, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L12A", "MUTATION_PDB": "L13A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.1, "dln(kf)_H2O": 0.04, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 12.0, "ddGKIN_H2O": -2.07, "mf": -1.8, "mu": 0.92, "CM_KIN": -2.76, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L12A", "MUTATION_PDB": "L13A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.1, "dln(kf)_H2O": 0.04, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 12.0, "ddGKIN_H2O": -2.07, "mf": -1.8, "mu": 0.92, "CM_KIN": -2.76, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K13A", "MUTATION_PDB": "K14A", "SEC_STR": "Helix", "RSA": "44.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.18, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 10.56, "ddGKIN_H2O": -3.51, "mf": -1.8, "mu": 0.92, "CM_KIN": -2.72, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K13A", "MUTATION_PDB": "K14A", "SEC_STR": "Helix", "RSA": "44.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.18, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 10.56, "ddGKIN_H2O": -3.51, "mf": -1.8, "mu": 0.92, "CM_KIN": -2.72, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K13G", "MUTATION_PDB": "K14G", "SEC_STR": "Helix", "RSA": "44.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.35, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 5.14, "dln(ku)_H2O": 3.06, "dGKIN_H2O": 5.2, "ddGKIN_H2O": -8.87, "mf": -2.26, "mu": 0.5, "CM_KIN": -2.76, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K13G", "MUTATION_PDB": "K14G", "SEC_STR": "Helix", "RSA": "44.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.35, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 5.14, "dln(ku)_H2O": 3.06, "dGKIN_H2O": 5.2, "ddGKIN_H2O": -8.87, "mf": -2.26, "mu": 0.5, "CM_KIN": -2.76, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D14A", "MUTATION_PDB": "D15A", "SEC_STR": "Helix", "RSA": "60.7", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D14A", "MUTATION_PDB": "D15A", "SEC_STR": "Helix", "RSA": "60.7", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L15A", "MUTATION_PDB": "L16A", "SEC_STR": "Helix", "RSA": "17.7", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.95, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 6.05, "ddGKIN_H2O": -8.02, "mf": -2.72, "mu": 0.33, "CM_KIN": -3.01, "PhiF_H2O": 0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L15A", "MUTATION_PDB": "L16A", "SEC_STR": "Helix", "RSA": "17.7", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 6.95, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 6.05, "ddGKIN_H2O": -8.02, "mf": -2.72, "mu": 0.33, "CM_KIN": -3.01, "PhiF_H2O": 0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L16A", "MUTATION_PDB": "L17A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.4, "dln(kf)_H2O": -0.66, "ln(ku)_H2O": 8.01, "dln(ku)_H2O": 5.93, "dGKIN_H2O": -1.44, "ddGKIN_H2O": -15.51, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L16A", "MUTATION_PDB": "L17A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.4, "dln(kf)_H2O": -0.66, "ln(ku)_H2O": 8.01, "dln(ku)_H2O": 5.93, "dGKIN_H2O": -1.44, "ddGKIN_H2O": -15.51, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K18A", "MUTATION_PDB": "K19A", "SEC_STR": "Helix", "RSA": "76.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K18A", "MUTATION_PDB": "K19A", "SEC_STR": "Helix", "RSA": "76.1", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "R19A", "MUTATION_PDB": "R20A", "SEC_STR": "Helix", "RSA": "33.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.22, "dln(kf)_H2O": -0.84, "ln(ku)_H2O": 7.38, "dln(ku)_H2O": 5.3, "dGKIN_H2O": -0.38, "ddGKIN_H2O": -14.45, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "R19A", "MUTATION_PDB": "R20A", "SEC_STR": "Helix", "RSA": "33.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.22, "dln(kf)_H2O": -0.84, "ln(ku)_H2O": 7.38, "dln(ku)_H2O": 5.3, "dGKIN_H2O": -0.38, "ddGKIN_H2O": -14.45, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S22A", "MUTATION_PDB": "S23A", "SEC_STR": "Coil", "RSA": "48.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.26, "dln(kf)_H2O": 0.2, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 9.13, "ddGKIN_H2O": -4.94, "mf": -2.05, "mu": 0.63, "CM_KIN": -2.68, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "S22A", "MUTATION_PDB": "S23A", "SEC_STR": "Coil", "RSA": "48.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.26, "dln(kf)_H2O": 0.2, "ln(ku)_H2O": 4.38, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 9.13, "ddGKIN_H2O": -4.94, "mf": -2.05, "mu": 0.63, "CM_KIN": -2.68, "PhiF_H2O": -0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V23A", "MUTATION_PDB": "V24A", "SEC_STR": "Coil", "RSA": "26.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.01, "dln(kf)_H2O": -0.05, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 10.16, "ddGKIN_H2O": -3.91, "mf": -1.8, "mu": 0.63, "CM_KIN": -2.43, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V23A", "MUTATION_PDB": "V24A", "SEC_STR": "Coil", "RSA": "26.8", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.01, "dln(kf)_H2O": -0.05, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 10.16, "ddGKIN_H2O": -3.91, "mf": -1.8, "mu": 0.63, "CM_KIN": -2.43, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K27A", "MUTATION_PDB": "K28A", "SEC_STR": "Helix", "RSA": "31.2", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "K27A", "MUTATION_PDB": "K28A", "SEC_STR": "Helix", "RSA": "31.2", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q32A", "MUTATION_PDB": "Q33A", "SEC_STR": "Helix", "RSA": "62.6", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "Q32A", "MUTATION_PDB": "Q33A", "SEC_STR": "Helix", "RSA": "62.6", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "R33A", "MUTATION_PDB": "R34A", "SEC_STR": "Helix", "RSA": "23.4", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.73, "dln(kf)_H2O": 0.67, "ln(ku)_H2O": 6.51, "dln(ku)_H2O": 4.43, "dGKIN_H2O": 5.22, "ddGKIN_H2O": -8.85, "mf": -2.47, "mu": 0.01, "CM_KIN": -2.47, "PhiF_H2O": -0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "R33A", "MUTATION_PDB": "R34A", "SEC_STR": "Helix", "RSA": "23.4", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 8.73, "dln(kf)_H2O": 0.67, "ln(ku)_H2O": 6.51, "dln(ku)_H2O": 4.43, "dGKIN_H2O": 5.22, "ddGKIN_H2O": -8.85, "mf": -2.47, "mu": 0.01, "CM_KIN": -2.47, "PhiF_H2O": -0.5, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L34A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 5.94, "dln(kf)_H2O": -2.12, "ln(ku)_H2O": 7.26, "dln(ku)_H2O": 5.18, "dGKIN_H2O": -3.11, "ddGKIN_H2O": -17.18, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "L34A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 5.94, "dln(kf)_H2O": -2.12, "ln(ku)_H2O": 7.26, "dln(ku)_H2O": 5.18, "dGKIN_H2O": -3.11, "ddGKIN_H2O": -17.18, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "I35A", "MUTATION_PDB": "I36A", "SEC_STR": "Helix", "RSA": "24.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.67, "dln(kf)_H2O": -0.39, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 10.99, "ddGKIN_H2O": -3.08, "mf": -1.51, "mu": 1.21, "CM_KIN": -2.72, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "I35A", "MUTATION_PDB": "I36A", "SEC_STR": "Helix", "RSA": "24.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.67, "dln(kf)_H2O": -0.39, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 10.99, "ddGKIN_H2O": -3.08, "mf": -1.51, "mu": 1.21, "CM_KIN": -2.72, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "I35V", "MUTATION_PDB": "I36V", "SEC_STR": "Helix", "RSA": "24.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.78, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 9.62, "ddGKIN_H2O": -4.45, "mf": -1.97, "mu": 0.42, "CM_KIN": -2.38, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "I35V", "MUTATION_PDB": "I36V", "SEC_STR": "Helix", "RSA": "24.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.78, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 3.69, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 9.62, "ddGKIN_H2O": -4.45, "mf": -1.97, "mu": 0.42, "CM_KIN": -2.38, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D37A", "MUTATION_PDB": "D38A", "SEC_STR": "Helix", "RSA": "12.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D37A", "MUTATION_PDB": "D38A", "SEC_STR": "Helix", "RSA": "12.9", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D38A", "MUTATION_PDB": "D39A", "SEC_STR": "Helix", "RSA": "39.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.82, "dln(kf)_H2O": -0.24, "ln(ku)_H2O": 6.06, "dln(ku)_H2O": 3.98, "dGKIN_H2O": 4.14, "ddGKIN_H2O": -9.93, "mf": -1.42, "mu": 0.5, "CM_KIN": -1.97, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D38A", "MUTATION_PDB": "D39A", "SEC_STR": "Helix", "RSA": "39.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.82, "dln(kf)_H2O": -0.24, "ln(ku)_H2O": 6.06, "dln(ku)_H2O": 3.98, "dGKIN_H2O": 4.14, "ddGKIN_H2O": -9.93, "mf": -1.42, "mu": 0.5, "CM_KIN": -1.97, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D38N", "MUTATION_PDB": "D39N", "SEC_STR": "Helix", "RSA": "39.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.86, "dln(kf)_H2O": -0.2, "ln(ku)_H2O": 6.49, "dln(ku)_H2O": 4.41, "dGKIN_H2O": 3.22, "ddGKIN_H2O": -10.85, "mf": -1.84, "mu": 0.46, "CM_KIN": -2.3, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "D38N", "MUTATION_PDB": "D39N", "SEC_STR": "Helix", "RSA": "39.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 7.86, "dln(kf)_H2O": -0.2, "ln(ku)_H2O": 6.49, "dln(ku)_H2O": 4.41, "dGKIN_H2O": 3.22, "ddGKIN_H2O": -10.85, "mf": -1.84, "mu": 0.46, "CM_KIN": -2.3, "PhiF_H2O": 0.1, "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "E39Q", "MUTATION_PDB": "E40Q", "SEC_STR": "Helix", "RSA": "65.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "E39Q", "MUTATION_PDB": "E40Q", "SEC_STR": "Helix", "RSA": "65.5", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "E40G", "MUTATION_PDB": "E41G", "SEC_STR": "Coil", "RSA": "54.6", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "E40G", "MUTATION_PDB": "E41G", "SEC_STR": "Coil", "RSA": "54.6", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E258G", "MUTATION_PDB": "E48G", "SEC_STR": "Helix", "RSA": "45.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.97, "dln(kf)_H2O": -0.51, "ln(ku)_H2O": 5.54, "dln(ku)_H2O": 2.32, "dGKIN_H2O": 13.47, "ddGKIN_H2O": -7.03, "mf": -5.61, "mu": 0.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.099", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E258G", "MUTATION_PDB": "E48G", "SEC_STR": "Helix", "RSA": "45.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.97, "dln(kf)_H2O": -0.51, "ln(ku)_H2O": 5.54, "dln(ku)_H2O": 2.32, "dGKIN_H2O": 13.47, "ddGKIN_H2O": -7.03, "mf": -5.61, "mu": 0.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.099", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A259G", "MUTATION_PDB": "A49G", "SEC_STR": "Helix", "RSA": "0.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.39, "dln(kf)_H2O": -1.09, "ln(ku)_H2O": 6.08, "dln(ku)_H2O": 2.86, "dGKIN_H2O": 10.71, "ddGKIN_H2O": -9.79, "mf": -6.28, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.100", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A259G", "MUTATION_PDB": "A49G", "SEC_STR": "Helix", "RSA": "0.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.39, "dln(kf)_H2O": -1.09, "ln(ku)_H2O": 6.08, "dln(ku)_H2O": 2.86, "dGKIN_H2O": 10.71, "ddGKIN_H2O": -9.79, "mf": -6.28, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.100", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "62.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.07, "dln(kf)_H2O": -0.41, "ln(ku)_H2O": 2.21, "dln(ku)_H2O": -1.01, "dGKIN_H2O": 21.97, "ddGKIN_H2O": 1.47, "mf": -4.23, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.101", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "62.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.07, "dln(kf)_H2O": -0.41, "ln(ku)_H2O": 2.21, "dln(ku)_H2O": -1.01, "dGKIN_H2O": 21.97, "ddGKIN_H2O": 1.47, "mf": -4.23, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.101", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "62.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.77, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 3.93, "dln(ku)_H2O": 0.71, "dGKIN_H2O": 16.95, "ddGKIN_H2O": -3.55, "mf": -4.27, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.102", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "62.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.77, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 3.93, "dln(ku)_H2O": 0.71, "dGKIN_H2O": 16.95, "ddGKIN_H2O": -3.55, "mf": -4.27, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.102", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.84, "dln(kf)_H2O": -0.64, "ln(ku)_H2O": 5.02, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 14.43, "ddGKIN_H2O": -6.07, "mf": -4.52, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.103", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.84, "dln(kf)_H2O": -0.64, "ln(ku)_H2O": 5.02, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 14.43, "ddGKIN_H2O": -6.07, "mf": -4.52, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.103", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262G", "MUTATION_PDB": "L52G", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.74, "dln(kf)_H2O": -0.74, "ln(ku)_H2O": 8.12, "dln(ku)_H2O": 4.9, "dGKIN_H2O": 6.49, "ddGKIN_H2O": -14.01, "mf": -5.19, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.104", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262G", "MUTATION_PDB": "L52G", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.74, "dln(kf)_H2O": -0.74, "ln(ku)_H2O": 8.12, "dln(ku)_H2O": 4.9, "dGKIN_H2O": 6.49, "ddGKIN_H2O": -14.01, "mf": -5.19, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.104", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "D264A", "MUTATION_PDB": "D54A", "SEC_STR": "Helix", "RSA": "63.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.41, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 2.67, "dln(ku)_H2O": -0.55, "dGKIN_H2O": 21.67, "ddGKIN_H2O": 1.17, "mf": -4.6, "mu": 1.26, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.105", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "D264A", "MUTATION_PDB": "D54A", "SEC_STR": "Helix", "RSA": "63.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.41, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 2.67, "dln(ku)_H2O": -0.55, "dGKIN_H2O": 21.67, "ddGKIN_H2O": 1.17, "mf": -4.6, "mu": 1.26, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.105", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "D264G", "MUTATION_PDB": "D54G", "SEC_STR": "Helix", "RSA": "63.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.37, "dln(kf)_H2O": -0.11, "ln(ku)_H2O": 5.28, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 15.1, "ddGKIN_H2O": -5.4, "mf": -4.9, "mu": 0.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.106", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "D264G", "MUTATION_PDB": "D54G", "SEC_STR": "Helix", "RSA": "63.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.37, "dln(kf)_H2O": -0.11, "ln(ku)_H2O": 5.28, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 15.1, "ddGKIN_H2O": -5.4, "mf": -4.9, "mu": 0.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.106", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A265G", "MUTATION_PDB": "A55G", "SEC_STR": "Helix", "RSA": "84.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.45, "dln(kf)_H2O": -0.03, "ln(ku)_H2O": 5.09, "dln(ku)_H2O": 1.87, "dGKIN_H2O": 15.77, "ddGKIN_H2O": -4.73, "mf": -5.02, "mu": 0.88, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.107", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A265G", "MUTATION_PDB": "A55G", "SEC_STR": "Helix", "RSA": "84.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.45, "dln(kf)_H2O": -0.03, "ln(ku)_H2O": 5.09, "dln(ku)_H2O": 1.87, "dGKIN_H2O": 15.77, "ddGKIN_H2O": -4.73, "mf": -5.02, "mu": 0.88, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.107", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(Y225W)", "MUTATION_PDB": "WT", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.48, "ln(ku)_H2O": 3.22, "dGKIN_H2O": 20.5, "mf": -4.81, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "BACKGROUND_MUT_PDB": [ "WT" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(Y225W)", "MUTATION_PDB": "WT", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.48, "ln(ku)_H2O": 3.22, "dGKIN_H2O": 20.5, "mf": -4.81, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "BACKGROUND_MUT_PDB": [ "WT" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q221A", "MUTATION_PDB": "Q11A", "SEC_STR": "Helix", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.2, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 2.59, "dln(ku)_H2O": -0.63, "dGKIN_H2O": 21.34, "ddGKIN_H2O": 0.84, "mf": -4.52, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q221A", "MUTATION_PDB": "Q11A", "SEC_STR": "Helix", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.2, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 2.59, "dln(ku)_H2O": -0.63, "dGKIN_H2O": 21.34, "ddGKIN_H2O": 0.84, "mf": -4.52, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q221G", "MUTATION_PDB": "Q11G", "SEC_STR": "Helix", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.77, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 3.11, "dln(ku)_H2O": -0.11, "dGKIN_H2O": 19.0, "ddGKIN_H2O": -1.5, "mf": -4.35, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q221G", "MUTATION_PDB": "Q11G", "SEC_STR": "Helix", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.77, "dln(kf)_H2O": -0.71, "ln(ku)_H2O": 3.11, "dln(ku)_H2O": -0.11, "dGKIN_H2O": 19.0, "ddGKIN_H2O": -1.5, "mf": -4.35, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "58.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.52, "dln(kf)_H2O": 0.04, "ln(ku)_H2O": 2.53, "dln(ku)_H2O": -0.69, "dGKIN_H2O": 22.3, "ddGKIN_H2O": 1.8, "mf": -4.77, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "58.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.52, "dln(kf)_H2O": 0.04, "ln(ku)_H2O": 2.53, "dln(ku)_H2O": -0.69, "dGKIN_H2O": 22.3, "ddGKIN_H2O": 1.8, "mf": -4.77, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "58.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.05, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 3.47, "dln(ku)_H2O": 0.25, "dGKIN_H2O": 18.79, "ddGKIN_H2O": -1.71, "mf": -4.6, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "58.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.05, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 3.47, "dln(ku)_H2O": 0.25, "dGKIN_H2O": 18.79, "ddGKIN_H2O": -1.71, "mf": -4.6, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A223G", "MUTATION_PDB": "A13G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.74, "dln(kf)_H2O": -0.74, "ln(ku)_H2O": 5.1, "dln(ku)_H2O": 1.88, "dGKIN_H2O": 13.97, "ddGKIN_H2O": -6.53, "mf": -4.98, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A223G", "MUTATION_PDB": "A13G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.74, "dln(kf)_H2O": -0.74, "ln(ku)_H2O": 5.1, "dln(ku)_H2O": 1.88, "dGKIN_H2O": 13.97, "ddGKIN_H2O": -6.53, "mf": -4.98, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F224A", "MUTATION_PDB": "F14A", "SEC_STR": "Helix", "RSA": "37.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.57, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 3.29, "dln(ku)_H2O": 0.07, "dGKIN_H2O": 18.03, "ddGKIN_H2O": -2.47, "mf": -4.98, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F224A", "MUTATION_PDB": "F14A", "SEC_STR": "Helix", "RSA": "37.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.57, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 3.29, "dln(ku)_H2O": 0.07, "dGKIN_H2O": 18.03, "ddGKIN_H2O": -2.47, "mf": -4.98, "mu": 0.84, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F224G", "MUTATION_PDB": "F14G", "SEC_STR": "Helix", "RSA": "37.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.2, "dln(kf)_H2O": -1.28, "ln(ku)_H2O": 5.41, "dln(ku)_H2O": 2.19, "dGKIN_H2O": 11.88, "ddGKIN_H2O": -8.62, "mf": -5.65, "mu": 0.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F224G", "MUTATION_PDB": "F14G", "SEC_STR": "Helix", "RSA": "37.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.2, "dln(kf)_H2O": -1.28, "ln(ku)_H2O": 5.41, "dln(ku)_H2O": 2.19, "dGKIN_H2O": 11.88, "ddGKIN_H2O": -8.62, "mf": -5.65, "mu": 0.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "39.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.66, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 3.03, "dln(ku)_H2O": -0.19, "dGKIN_H2O": 18.91, "ddGKIN_H2O": -1.59, "mf": -3.97, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "39.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.66, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 3.03, "dln(ku)_H2O": -0.19, "dGKIN_H2O": 18.91, "ddGKIN_H2O": -1.59, "mf": -3.97, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "39.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.59, "dln(kf)_H2O": -0.89, "ln(ku)_H2O": 4.16, "dln(ku)_H2O": 0.94, "dGKIN_H2O": 15.94, "ddGKIN_H2O": -4.56, "mf": -4.9, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "39.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.59, "dln(kf)_H2O": -0.89, "ln(ku)_H2O": 4.16, "dln(ku)_H2O": 0.94, "dGKIN_H2O": 15.94, "ddGKIN_H2O": -4.56, "mf": -4.9, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.13, "dln(kf)_H2O": -1.35, "ln(ku)_H2O": 3.13, "dln(ku)_H2O": -0.09, "dGKIN_H2O": 13.18, "ddGKIN_H2O": -7.32, "mf": -4.6, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.13, "dln(kf)_H2O": -1.35, "ln(ku)_H2O": 3.13, "dln(ku)_H2O": -0.09, "dGKIN_H2O": 13.18, "ddGKIN_H2O": -7.32, "mf": -4.6, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228A", "MUTATION_PDB": "L18A", "SEC_STR": "Helix", "RSA": "33.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -1.44, "ln(ku)_H2O": 2.46, "dln(ku)_H2O": -0.76, "dGKIN_H2O": 18.79, "ddGKIN_H2O": -1.71, "mf": -4.39, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228A", "MUTATION_PDB": "L18A", "SEC_STR": "Helix", "RSA": "33.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -1.44, "ln(ku)_H2O": 2.46, "dln(ku)_H2O": -0.76, "dGKIN_H2O": 18.79, "ddGKIN_H2O": -1.71, "mf": -4.39, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Helix", "RSA": "33.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.78, "dln(kf)_H2O": -1.7, "ln(ku)_H2O": 3.26, "dln(ku)_H2O": 0.04, "dGKIN_H2O": 16.15, "ddGKIN_H2O": -4.35, "mf": -5.23, "mu": 1.63, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Helix", "RSA": "33.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.78, "dln(kf)_H2O": -1.7, "ln(ku)_H2O": 3.26, "dln(ku)_H2O": 0.04, "dGKIN_H2O": 16.15, "ddGKIN_H2O": -4.35, "mf": -5.23, "mu": 1.63, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L20A", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.18, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 7.17, "dln(ku)_H2O": 3.95, "dGKIN_H2O": 9.92, "ddGKIN_H2O": -10.58, "mf": -5.61, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L20A", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.18, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 7.17, "dln(ku)_H2O": 3.95, "dGKIN_H2O": 9.92, "ddGKIN_H2O": -10.58, "mf": -5.61, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230G", "MUTATION_PDB": "L20G", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -1.44, "ln(ku)_H2O": 9.68, "dln(ku)_H2O": 6.46, "dGKIN_H2O": 0.88, "ddGKIN_H2O": -19.62, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230G", "MUTATION_PDB": "L20G", "SEC_STR": "Coil", "RSA": "4.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.04, "dln(kf)_H2O": -1.44, "ln(ku)_H2O": 9.68, "dln(ku)_H2O": 6.46, "dGKIN_H2O": 0.88, "ddGKIN_H2O": -19.62, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "P231G", "MUTATION_PDB": "P21G", "SEC_STR": "Coil", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.98, "dln(kf)_H2O": -0.5, "ln(ku)_H2O": 4.26, "dln(ku)_H2O": 1.04, "dGKIN_H2O": 16.65, "ddGKIN_H2O": -3.85, "mf": -4.44, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "P231G", "MUTATION_PDB": "P21G", "SEC_STR": "Coil", "RSA": "58.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.98, "dln(kf)_H2O": -0.5, "ln(ku)_H2O": 4.26, "dln(ku)_H2O": 1.04, "dGKIN_H2O": 16.65, "ddGKIN_H2O": -3.85, "mf": -4.44, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N232G", "MUTATION_PDB": "N22G", "SEC_STR": "Coil", "RSA": "32.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.7, "dln(kf)_H2O": 0.22, "ln(ku)_H2O": 6.28, "dln(ku)_H2O": 3.06, "dGKIN_H2O": 13.43, "ddGKIN_H2O": -7.07, "mf": -4.81, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N232G", "MUTATION_PDB": "N22G", "SEC_STR": "Coil", "RSA": "32.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.7, "dln(kf)_H2O": 0.22, "ln(ku)_H2O": 6.28, "dln(ku)_H2O": 3.06, "dGKIN_H2O": 13.43, "ddGKIN_H2O": -7.07, "mf": -4.81, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L233A", "MUTATION_PDB": "L23A", "SEC_STR": "Coil", "RSA": "4.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.17, "dln(kf)_H2O": -2.31, "ln(ku)_H2O": 9.17, "dln(ku)_H2O": 5.95, "ddGKIN_H2O": -20.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L233A", "MUTATION_PDB": "L23A", "SEC_STR": "Coil", "RSA": "4.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.17, "dln(kf)_H2O": -2.31, "ln(ku)_H2O": 9.17, "dln(ku)_H2O": 5.95, "ddGKIN_H2O": -20.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E236A", "MUTATION_PDB": "E26A", "SEC_STR": "Helix", "RSA": "78.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.51, "dln(kf)_H2O": 0.03, "ln(ku)_H2O": 3.15, "dln(ku)_H2O": -0.07, "dGKIN_H2O": 20.71, "ddGKIN_H2O": 0.21, "mf": -4.9, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E236A", "MUTATION_PDB": "E26A", "SEC_STR": "Helix", "RSA": "78.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.51, "dln(kf)_H2O": 0.03, "ln(ku)_H2O": 3.15, "dln(ku)_H2O": -0.07, "dGKIN_H2O": 20.71, "ddGKIN_H2O": 0.21, "mf": -4.9, "mu": 1.05, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E236G", "MUTATION_PDB": "E26G", "SEC_STR": "Helix", "RSA": "78.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.72, "dln(kf)_H2O": -1.76, "ln(ku)_H2O": 2.19, "dln(ku)_H2O": -1.03, "dGKIN_H2O": 18.66, "ddGKIN_H2O": -1.84, "mf": -4.23, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.063", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E236G", "MUTATION_PDB": "E26G", "SEC_STR": "Helix", "RSA": "78.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.72, "dln(kf)_H2O": -1.76, "ln(ku)_H2O": 2.19, "dln(ku)_H2O": -1.03, "dGKIN_H2O": 18.66, "ddGKIN_H2O": -1.84, "mf": -4.23, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.063", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q237A", "MUTATION_PDB": "Q27A", "SEC_STR": "Helix", "RSA": "39.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.78, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 5.47, "dln(ku)_H2O": 2.25, "dGKIN_H2O": 13.18, "ddGKIN_H2O": -7.32, "mf": -4.69, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.064", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q237A", "MUTATION_PDB": "Q27A", "SEC_STR": "Helix", "RSA": "39.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.78, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 5.47, "dln(ku)_H2O": 2.25, "dGKIN_H2O": 13.18, "ddGKIN_H2O": -7.32, "mf": -4.69, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.064", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q237G", "MUTATION_PDB": "Q27G", "SEC_STR": "Helix", "RSA": "39.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.48, "dln(kf)_H2O": -2.0, "ln(ku)_H2O": 6.92, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 6.36, "ddGKIN_H2O": -14.14, "mf": -6.19, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.065", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q237G", "MUTATION_PDB": "Q27G", "SEC_STR": "Helix", "RSA": "39.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.48, "dln(kf)_H2O": -2.0, "ln(ku)_H2O": 6.92, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 6.36, "ddGKIN_H2O": -14.14, "mf": -6.19, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.065", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "18.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.33, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 15.06, "ddGKIN_H2O": -5.44, "mf": -4.31, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.066", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "18.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.33, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 4.25, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 15.06, "ddGKIN_H2O": -5.44, "mf": -4.31, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.066", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "18.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.77, "dln(kf)_H2O": -2.71, "ln(ku)_H2O": 5.41, "dln(ku)_H2O": 2.19, "dGKIN_H2O": 8.33, "ddGKIN_H2O": -12.17, "mf": -5.77, "mu": 0.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.067", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "18.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.77, "dln(kf)_H2O": -2.71, "ln(ku)_H2O": 5.41, "dln(ku)_H2O": 2.19, "dGKIN_H2O": 8.33, "ddGKIN_H2O": -12.17, "mf": -5.77, "mu": 0.5, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.067", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N239A", "MUTATION_PDB": "N29A", "SEC_STR": "Helix", "RSA": "52.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.57, "dln(kf)_H2O": 0.09, "ln(ku)_H2O": 2.56, "dln(ku)_H2O": -0.66, "dGKIN_H2O": 22.34, "ddGKIN_H2O": 1.84, "mf": -4.56, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.068", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N239A", "MUTATION_PDB": "N29A", "SEC_STR": "Helix", "RSA": "52.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.57, "dln(kf)_H2O": 0.09, "ln(ku)_H2O": 2.56, "dln(ku)_H2O": -0.66, "dGKIN_H2O": 22.34, "ddGKIN_H2O": 1.84, "mf": -4.56, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.068", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N239G", "MUTATION_PDB": "N29G", "SEC_STR": "Helix", "RSA": "52.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.09, "dln(kf)_H2O": -1.39, "ln(ku)_H2O": 2.39, "dln(ku)_H2O": -0.83, "dGKIN_H2O": 19.08, "ddGKIN_H2O": -1.42, "mf": -4.85, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.069", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N239G", "MUTATION_PDB": "N29G", "SEC_STR": "Helix", "RSA": "52.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.09, "dln(kf)_H2O": -1.39, "ln(ku)_H2O": 2.39, "dln(ku)_H2O": -0.83, "dGKIN_H2O": 19.08, "ddGKIN_H2O": -1.42, "mf": -4.85, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.069", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240A", "MUTATION_PDB": "G30A", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.53, "dln(kf)_H2O": 1.05, "ln(ku)_H2O": 3.45, "dln(ku)_H2O": 0.23, "dGKIN_H2O": 23.14, "ddGKIN_H2O": 2.64, "mf": -4.39, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.070", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240A", "MUTATION_PDB": "G30A", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.53, "dln(kf)_H2O": 1.05, "ln(ku)_H2O": 3.45, "dln(ku)_H2O": 0.23, "dGKIN_H2O": 23.14, "ddGKIN_H2O": 2.64, "mf": -4.39, "mu": 1.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.070", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240S", "MUTATION_PDB": "G30S", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.9, "dln(kf)_H2O": 0.42, "ln(ku)_H2O": 3.27, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 22.18, "ddGKIN_H2O": 1.68, "mf": -4.35, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.071", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240S", "MUTATION_PDB": "G30S", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.9, "dln(kf)_H2O": 0.42, "ln(ku)_H2O": 3.27, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 22.18, "ddGKIN_H2O": 1.68, "mf": -4.35, "mu": 1.09, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.071", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240T", "MUTATION_PDB": "G30T", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.5, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 3.08, "dln(ku)_H2O": -0.14, "dGKIN_H2O": 21.25, "ddGKIN_H2O": 0.75, "mf": -4.31, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.072", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240T", "MUTATION_PDB": "G30T", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.5, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 3.08, "dln(ku)_H2O": -0.14, "dGKIN_H2O": 21.25, "ddGKIN_H2O": 0.75, "mf": -4.31, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.072", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240I", "MUTATION_PDB": "G30I", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.1, "dln(kf)_H2O": 0.62, "ln(ku)_H2O": 4.23, "dln(ku)_H2O": 1.01, "dGKIN_H2O": 20.75, "ddGKIN_H2O": 0.25, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.073", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240I", "MUTATION_PDB": "G30I", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.1, "dln(kf)_H2O": 0.62, "ln(ku)_H2O": 4.23, "dln(ku)_H2O": 1.01, "dGKIN_H2O": 20.75, "ddGKIN_H2O": 0.25, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.073", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240V", "MUTATION_PDB": "G30V", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.69, "dln(kf)_H2O": 0.21, "ln(ku)_H2O": 3.19, "dln(ku)_H2O": -0.03, "dGKIN_H2O": 20.88, "ddGKIN_H2O": 0.38, "mf": -4.48, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.074", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "G240V", "MUTATION_PDB": "G30V", "SEC_STR": "Helix", "RSA": "39.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.69, "dln(kf)_H2O": 0.21, "ln(ku)_H2O": 3.19, "dln(ku)_H2O": -0.03, "dGKIN_H2O": 20.88, "ddGKIN_H2O": 0.38, "mf": -4.48, "mu": 1.46, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.074", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F241A", "MUTATION_PDB": "F31A", "SEC_STR": "Helix", "RSA": "7.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.12, "dln(kf)_H2O": -1.36, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 3.13, "dGKIN_H2O": 9.37, "ddGKIN_H2O": -11.13, "mf": -7.2, "mu": 1.17, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.075", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F241A", "MUTATION_PDB": "F31A", "SEC_STR": "Helix", "RSA": "7.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.12, "dln(kf)_H2O": -1.36, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 3.13, "dGKIN_H2O": 9.37, "ddGKIN_H2O": -11.13, "mf": -7.2, "mu": 1.17, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.075", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F241G", "MUTATION_PDB": "F31G", "SEC_STR": "Helix", "RSA": "7.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.33, "dln(kf)_H2O": -4.15, "ln(ku)_H2O": 6.89, "dln(ku)_H2O": 3.67, "dGKIN_H2O": 1.09, "ddGKIN_H2O": -19.41, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.076", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "F241G", "MUTATION_PDB": "F31G", "SEC_STR": "Helix", "RSA": "7.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.33, "dln(kf)_H2O": -4.15, "ln(ku)_H2O": 6.89, "dln(ku)_H2O": 3.67, "dGKIN_H2O": 1.09, "ddGKIN_H2O": -19.41, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.076", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.21, "dln(kf)_H2O": -1.27, "ln(ku)_H2O": 3.91, "dln(ku)_H2O": 0.69, "dGKIN_H2O": 15.61, "ddGKIN_H2O": -4.89, "mf": -4.56, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.077", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.21, "dln(kf)_H2O": -1.27, "ln(ku)_H2O": 3.91, "dln(ku)_H2O": 0.69, "dGKIN_H2O": 15.61, "ddGKIN_H2O": -4.89, "mf": -4.56, "mu": 1.34, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.077", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242A", "MUTATION_PDB": "I32A", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.1, "dln(kf)_H2O": -1.38, "ln(ku)_H2O": 4.76, "dln(ku)_H2O": 1.54, "dGKIN_H2O": 13.22, "ddGKIN_H2O": -7.28, "mf": -5.4, "mu": 0.67, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.078", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242A", "MUTATION_PDB": "I32A", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.1, "dln(kf)_H2O": -1.38, "ln(ku)_H2O": 4.76, "dln(ku)_H2O": 1.54, "dGKIN_H2O": 13.22, "ddGKIN_H2O": -7.28, "mf": -5.4, "mu": 0.67, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.078", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242G", "MUTATION_PDB": "I32G", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.83, "dln(kf)_H2O": -2.65, "ln(ku)_H2O": 4.89, "dln(ku)_H2O": 1.67, "dGKIN_H2O": 9.75, "ddGKIN_H2O": -10.75, "mf": -6.57, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.079", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242G", "MUTATION_PDB": "I32G", "SEC_STR": "Helix", "RSA": "8.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.83, "dln(kf)_H2O": -2.65, "ln(ku)_H2O": 4.89, "dln(ku)_H2O": 1.67, "dGKIN_H2O": 9.75, "ddGKIN_H2O": -10.75, "mf": -6.57, "mu": 1.0, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.079", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q243A", "MUTATION_PDB": "Q33A", "SEC_STR": "Helix", "RSA": "59.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.95, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 2.17, "dln(ku)_H2O": -1.05, "dGKIN_H2O": 21.76, "ddGKIN_H2O": 1.26, "mf": -4.48, "mu": 1.8, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.080", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q243A", "MUTATION_PDB": "Q33A", "SEC_STR": "Helix", "RSA": "59.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.95, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 2.17, "dln(ku)_H2O": -1.05, "dGKIN_H2O": 21.76, "ddGKIN_H2O": 1.26, "mf": -4.48, "mu": 1.8, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.080", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q243G", "MUTATION_PDB": "Q33G", "SEC_STR": "Helix", "RSA": "59.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.6, "dln(kf)_H2O": -1.88, "ln(ku)_H2O": 2.04, "dln(ku)_H2O": -1.18, "dGKIN_H2O": 18.74, "ddGKIN_H2O": -1.76, "mf": -4.64, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.081", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "Q243G", "MUTATION_PDB": "Q33G", "SEC_STR": "Helix", "RSA": "59.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.6, "dln(kf)_H2O": -1.88, "ln(ku)_H2O": 2.04, "dln(ku)_H2O": -1.18, "dGKIN_H2O": 18.74, "ddGKIN_H2O": -1.76, "mf": -4.64, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.081", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "36.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.81, "dln(kf)_H2O": 0.33, "ln(ku)_H2O": 3.18, "dln(ku)_H2O": -0.04, "dGKIN_H2O": 21.42, "ddGKIN_H2O": 0.92, "mf": -4.56, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.082", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "36.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.81, "dln(kf)_H2O": 0.33, "ln(ku)_H2O": 3.18, "dln(ku)_H2O": -0.04, "dGKIN_H2O": 21.42, "ddGKIN_H2O": 0.92, "mf": -4.56, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.082", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "36.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.61, "dln(kf)_H2O": -0.87, "ln(ku)_H2O": 3.68, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 17.15, "ddGKIN_H2O": -3.35, "mf": -4.94, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.083", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "36.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.61, "dln(kf)_H2O": -0.87, "ln(ku)_H2O": 3.68, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 17.15, "ddGKIN_H2O": -3.35, "mf": -4.94, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.083", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.83, "dln(kf)_H2O": -1.65, "ln(ku)_H2O": 5.29, "dln(ku)_H2O": 2.07, "dGKIN_H2O": 11.25, "ddGKIN_H2O": -9.25, "mf": -5.31, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.084", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.83, "dln(kf)_H2O": -1.65, "ln(ku)_H2O": 5.29, "dln(ku)_H2O": 2.07, "dGKIN_H2O": 11.25, "ddGKIN_H2O": -9.25, "mf": -5.31, "mu": 0.79, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.084", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245G", "MUTATION_PDB": "L35G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.36, "dln(kf)_H2O": -2.12, "ln(ku)_H2O": 5.75, "dln(ku)_H2O": 2.53, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -11.55, "mf": -7.49, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.085", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245G", "MUTATION_PDB": "L35G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.36, "dln(kf)_H2O": -2.12, "ln(ku)_H2O": 5.75, "dln(ku)_H2O": 2.53, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -11.55, "mf": -7.49, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.085", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "P249G", "MUTATION_PDB": "P39G", "SEC_STR": "Helix", "RSA": "25.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -1.07, "ln(ku)_H2O": 4.87, "dln(ku)_H2O": 1.65, "dGKIN_H2O": 13.72, "ddGKIN_H2O": -6.78, "mf": -4.94, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.086", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "P249G", "MUTATION_PDB": "P39G", "SEC_STR": "Helix", "RSA": "25.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -1.07, "ln(ku)_H2O": 4.87, "dln(ku)_H2O": 1.65, "dGKIN_H2O": 13.72, "ddGKIN_H2O": -6.78, "mf": -4.94, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.086", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Helix", "RSA": "83.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.88, "dln(kf)_H2O": -0.6, "ln(ku)_H2O": 4.01, "dln(ku)_H2O": 0.79, "dGKIN_H2O": 17.03, "ddGKIN_H2O": -3.47, "mf": -4.81, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.087", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Helix", "RSA": "83.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.88, "dln(kf)_H2O": -0.6, "ln(ku)_H2O": 4.01, "dln(ku)_H2O": 0.79, "dGKIN_H2O": 17.03, "ddGKIN_H2O": -3.47, "mf": -4.81, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.087", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "10.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.84, "dln(kf)_H2O": 0.36, "ln(ku)_H2O": 3.11, "dln(ku)_H2O": -0.11, "dGKIN_H2O": 21.63, "ddGKIN_H2O": 1.13, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.088", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "10.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.84, "dln(kf)_H2O": 0.36, "ln(ku)_H2O": 3.11, "dln(ku)_H2O": -0.11, "dGKIN_H2O": 21.63, "ddGKIN_H2O": 1.13, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.088", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "10.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.95, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 4.21, "dln(ku)_H2O": 0.99, "dGKIN_H2O": 16.69, "ddGKIN_H2O": -3.81, "mf": -4.52, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.089", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "10.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.95, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 4.21, "dln(ku)_H2O": 0.99, "dGKIN_H2O": 16.69, "ddGKIN_H2O": -3.81, "mf": -4.52, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.089", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A253G", "MUTATION_PDB": "A43G", "SEC_STR": "Helix", "RSA": "65.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.9, "dln(kf)_H2O": -0.58, "ln(ku)_H2O": 4.08, "dln(ku)_H2O": 0.86, "dGKIN_H2O": 16.95, "ddGKIN_H2O": -3.55, "mf": -4.85, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.090", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A253G", "MUTATION_PDB": "A43G", "SEC_STR": "Helix", "RSA": "65.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.9, "dln(kf)_H2O": -0.58, "ln(ku)_H2O": 4.08, "dln(ku)_H2O": 0.86, "dGKIN_H2O": 16.95, "ddGKIN_H2O": -3.55, "mf": -4.85, "mu": 0.75, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.090", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N254A", "MUTATION_PDB": "N44A", "SEC_STR": "Helix", "RSA": "65.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.43, "dln(kf)_H2O": -1.05, "ln(ku)_H2O": 1.35, "dln(ku)_H2O": -1.87, "dGKIN_H2O": 22.51, "ddGKIN_H2O": 2.01, "mf": -3.72, "mu": 1.72, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.091", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N254A", "MUTATION_PDB": "N44A", "SEC_STR": "Helix", "RSA": "65.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.43, "dln(kf)_H2O": -1.05, "ln(ku)_H2O": 1.35, "dln(ku)_H2O": -1.87, "dGKIN_H2O": 22.51, "ddGKIN_H2O": 2.01, "mf": -3.72, "mu": 1.72, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.091", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N254G", "MUTATION_PDB": "N44G", "SEC_STR": "Helix", "RSA": "65.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.65, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 3.67, "dln(ku)_H2O": 0.45, "dGKIN_H2O": 17.28, "ddGKIN_H2O": -3.22, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.092", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N254G", "MUTATION_PDB": "N44G", "SEC_STR": "Helix", "RSA": "65.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.65, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 3.67, "dln(ku)_H2O": 0.45, "dGKIN_H2O": 17.28, "ddGKIN_H2O": -3.22, "mf": -4.56, "mu": 0.92, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.092", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "7.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": -1.41, "ln(ku)_H2O": 4.19, "dln(ku)_H2O": 0.97, "dGKIN_H2O": 14.56, "ddGKIN_H2O": -5.94, "mf": -4.56, "mu": 1.42, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.093", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "7.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": -1.41, "ln(ku)_H2O": 4.19, "dln(ku)_H2O": 0.97, "dGKIN_H2O": 14.56, "ddGKIN_H2O": -5.94, "mf": -4.56, "mu": 1.42, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.093", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255G", "MUTATION_PDB": "L45G", "SEC_STR": "Helix", "RSA": "7.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.71, "dln(kf)_H2O": -1.77, "ln(ku)_H2O": 7.03, "dln(ku)_H2O": 3.81, "dGKIN_H2O": 6.65, "ddGKIN_H2O": -13.85, "mf": -7.45, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.094", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255G", "MUTATION_PDB": "L45G", "SEC_STR": "Helix", "RSA": "7.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.71, "dln(kf)_H2O": -1.77, "ln(ku)_H2O": 7.03, "dln(ku)_H2O": 3.81, "dGKIN_H2O": 6.65, "ddGKIN_H2O": -13.85, "mf": -7.45, "mu": 0.54, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.094", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L256A", "MUTATION_PDB": "L46A", "SEC_STR": "Helix", "RSA": "26.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.75, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 4.47, "dln(ku)_H2O": 1.25, "dGKIN_H2O": 15.56, "ddGKIN_H2O": -4.94, "mf": -5.56, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.095", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L256A", "MUTATION_PDB": "L46A", "SEC_STR": "Helix", "RSA": "26.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.75, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 4.47, "dln(ku)_H2O": 1.25, "dGKIN_H2O": 15.56, "ddGKIN_H2O": -4.94, "mf": -5.56, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.095", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L256G", "MUTATION_PDB": "L46G", "SEC_STR": "Helix", "RSA": "26.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.94, "dln(kf)_H2O": -1.54, "ln(ku)_H2O": 6.41, "dln(ku)_H2O": 3.19, "dGKIN_H2O": 8.74, "ddGKIN_H2O": -11.76, "mf": -7.45, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.096", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L256G", "MUTATION_PDB": "L46G", "SEC_STR": "Helix", "RSA": "26.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.94, "dln(kf)_H2O": -1.54, "ln(ku)_H2O": 6.41, "dln(ku)_H2O": 3.19, "dGKIN_H2O": 8.74, "ddGKIN_H2O": -11.76, "mf": -7.45, "mu": 0.71, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.096", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A257G", "MUTATION_PDB": "A47G", "SEC_STR": "Helix", "RSA": "38.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.24, "dln(kf)_H2O": -1.24, "ln(ku)_H2O": 3.95, "dln(ku)_H2O": 0.73, "dGKIN_H2O": 15.61, "ddGKIN_H2O": -4.89, "mf": -4.48, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.097", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "A257G", "MUTATION_PDB": "A47G", "SEC_STR": "Helix", "RSA": "38.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.24, "dln(kf)_H2O": -1.24, "ln(ku)_H2O": 3.95, "dln(ku)_H2O": 0.73, "dGKIN_H2O": 15.61, "ddGKIN_H2O": -4.89, "mf": -4.48, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.097", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E258A", "MUTATION_PDB": "E48A", "SEC_STR": "Helix", "RSA": "45.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.08, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": -0.22, "dGKIN_H2O": 20.0, "ddGKIN_H2O": -0.5, "mf": -4.44, "mu": 1.17, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.098", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (Y17W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 62.0, "UniProt": "P38507", "PDB_wild": "1ss1", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1ss1A00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E258A", "MUTATION_PDB": "E48A", "SEC_STR": "Helix", "RSA": "45.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.08, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 3.0, "dln(ku)_H2O": -0.22, "dGKIN_H2O": 20.0, "ddGKIN_H2O": -0.5, "mf": -4.44, "mu": 1.17, "BACKGROUND_MUT_UNIPROT": [ "Y225W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.098", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_PDB": "WT(D17E,Y28K,E43W)", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.96, "ln(ku)_H2O": 0.88, "dGKIN_H2O": 20.02, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_PDB": "WT(D17E,Y28K,E43W)", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.96, "ln(ku)_H2O": 0.88, "dGKIN_H2O": 20.02, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "19.5", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.39, "dln(kf)_H2O": -0.57, "ln(ku)_H2O": 1.63, "dln(ku)_H2O": 0.75, "dGKIN_H2O": 16.75, "ddGKIN_H2O": -3.27, "PhiF_H2O": 0.43, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "19.5", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.39, "dln(kf)_H2O": -0.57, "ln(ku)_H2O": 1.63, "dln(ku)_H2O": 0.75, "dGKIN_H2O": 16.75, "ddGKIN_H2O": -3.27, "PhiF_H2O": 0.43, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.68, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 4.58, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 10.16, "ddGKIN_H2O": -9.86, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.68, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 4.58, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 10.16, "ddGKIN_H2O": -9.86, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 9.05, "dln(kf)_H2O": 0.09, "ln(ku)_H2O": 1.31, "dln(ku)_H2O": 0.43, "dGKIN_H2O": 19.18, "ddGKIN_H2O": -0.84, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 9.05, "dln(kf)_H2O": 0.09, "ln(ku)_H2O": 1.31, "dln(ku)_H2O": 0.43, "dGKIN_H2O": 19.18, "ddGKIN_H2O": -0.84, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "10.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.68, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 0.88, "dGKIN_H2O": 19.33, "ddGKIN_H2O": -0.69, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "10.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.68, "dln(kf)_H2O": -0.28, "ln(ku)_H2O": 0.88, "dGKIN_H2O": 19.33, "ddGKIN_H2O": -0.69, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "41.0", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.41, "dln(kf)_H2O": -0.55, "ln(ku)_H2O": 1.22, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 17.81, "ddGKIN_H2O": -2.21, "PhiF_H2O": 0.61, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "41.0", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.41, "dln(kf)_H2O": -0.55, "ln(ku)_H2O": 1.22, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 17.81, "ddGKIN_H2O": -2.21, "PhiF_H2O": 0.61, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "10.7", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.24, "dln(kf)_H2O": -0.72, "ln(ku)_H2O": 1.44, "dln(ku)_H2O": 0.56, "dGKIN_H2O": 16.85, "ddGKIN_H2O": -3.17, "PhiF_H2O": 0.56, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "10.7", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.24, "dln(kf)_H2O": -0.72, "ln(ku)_H2O": 1.44, "dln(ku)_H2O": 0.56, "dGKIN_H2O": 16.85, "ddGKIN_H2O": -3.17, "PhiF_H2O": 0.56, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L818A", "MUTATION_PDB": "L32A", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.58, "dln(kf)_H2O": -0.38, "ln(ku)_H2O": 1.55, "dln(ku)_H2O": 0.67, "dGKIN_H2O": 17.42, "ddGKIN_H2O": -2.6, "PhiF_H2O": 0.37, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L818A", "MUTATION_PDB": "L32A", "SEC_STR": "Helix", "RSA": "34.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.58, "dln(kf)_H2O": -0.38, "ln(ku)_H2O": 1.55, "dln(ku)_H2O": 0.67, "dGKIN_H2O": 17.42, "ddGKIN_H2O": -2.6, "PhiF_H2O": 0.37, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "30.2", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.1, "dln(kf)_H2O": -0.86, "ln(ku)_H2O": 2.77, "dln(ku)_H2O": 1.89, "dGKIN_H2O": 13.21, "ddGKIN_H2O": -6.81, "PhiF_H2O": 0.31, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "30.2", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.1, "dln(kf)_H2O": -0.86, "ln(ku)_H2O": 2.77, "dln(ku)_H2O": 1.89, "dGKIN_H2O": 13.21, "ddGKIN_H2O": -6.81, "PhiF_H2O": 0.31, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "64.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 9.01, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 1.34, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 19.0, "ddGKIN_H2O": -1.02, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "64.1", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 9.01, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 1.34, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 19.0, "ddGKIN_H2O": -1.02, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Coil", "RSA": "26.8", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.95, "dln(kf)_H2O": -0.01, "ln(ku)_H2O": 3.81, "dln(ku)_H2O": 2.93, "dGKIN_H2O": 12.73, "ddGKIN_H2O": -7.29, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Coil", "RSA": "26.8", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.95, "dln(kf)_H2O": -0.01, "ln(ku)_H2O": 3.81, "dln(ku)_H2O": 2.93, "dGKIN_H2O": 12.73, "ddGKIN_H2O": -7.29, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.19, "dln(kf)_H2O": -0.77, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 4.47, "dGKIN_H2O": 7.04, "ddGKIN_H2O": -12.98, "PhiF_H2O": 0.15, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.19, "dln(kf)_H2O": -0.77, "ln(ku)_H2O": 5.35, "dln(ku)_H2O": 4.47, "dGKIN_H2O": 7.04, "ddGKIN_H2O": -12.98, "PhiF_H2O": 0.15, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "32.3", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.14, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 2.03, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 15.14, "ddGKIN_H2O": -4.88, "PhiF_H2O": 0.42, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "32.3", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.14, "dln(kf)_H2O": -0.82, "ln(ku)_H2O": 2.03, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 15.14, "ddGKIN_H2O": -4.88, "PhiF_H2O": 0.42, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I835A", "MUTATION_PDB": "I49A", "SEC_STR": "Helix", "RSA": "20.7", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.81, "dln(kf)_H2O": -0.15, "ln(ku)_H2O": 1.95, "dln(ku)_H2O": 1.07, "dGKIN_H2O": 17.0, "ddGKIN_H2O": -3.02, "PhiF_H2O": 0.12, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "I835A", "MUTATION_PDB": "I49A", "SEC_STR": "Helix", "RSA": "20.7", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.81, "dln(kf)_H2O": -0.15, "ln(ku)_H2O": 1.95, "dln(ku)_H2O": 1.07, "dGKIN_H2O": 17.0, "ddGKIN_H2O": -3.02, "PhiF_H2O": 0.12, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L836A", "MUTATION_PDB": "L50A", "SEC_STR": "Helix", "RSA": "21.3", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.54, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 3.04, "dln(ku)_H2O": 2.16, "dGKIN_H2O": 13.63, "ddGKIN_H2O": -6.39, "PhiF_H2O": 0.16, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(D27E/Y28K/E43W)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "UniProt": "Q51918", "PDB_wild": "2fs1", "MUTATED_CHAIN": "A", "PFAM": [ "PF17573" ], "CATH": [ "2fs1A00 (1.10.8.40)" ], "MUTATION_UNIPROT": "L836A", "MUTATION_PDB": "L50A", "SEC_STR": "Helix", "RSA": "21.3", "T": 25.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "GndHCl", "ln(kf)_H2O": 8.54, "dln(kf)_H2O": -0.42, "ln(ku)_H2O": 3.04, "dln(ku)_H2O": 2.16, "dGKIN_H2O": 13.63, "ddGKIN_H2O": -6.39, "PhiF_H2O": 0.16, "BACKGROUND_MUT_PDB": [ "D17E", "Y28K", "E43W" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT(T51K,F52A)", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.81, "ln(ku)_H2O": 1.34, "dGKIN_H2O": 16.03, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT(T51K,F52A)", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.81, "ln(ku)_H2O": 1.34, "dGKIN_H2O": 16.03, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "23.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.9, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 0.88, "dln(ku)_H2O": -0.46, "dGKIN_H2O": 14.91, "ddGKIN_H2O": -1.12, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "23.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.9, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 0.88, "dln(ku)_H2O": -0.46, "dGKIN_H2O": 14.91, "ddGKIN_H2O": -1.12, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "1.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.34, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 4.91, "dln(ku)_H2O": 3.57, "dGKIN_H2O": 6.02, "ddGKIN_H2O": -10.01, "PhiF_H2O": 0.12, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "1.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.34, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 4.91, "dln(ku)_H2O": 3.57, "dGKIN_H2O": 6.02, "ddGKIN_H2O": -10.01, "PhiF_H2O": 0.12, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "SEC_STR": "Helix", "RSA": "26.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.0, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 2.64, "dln(ku)_H2O": 1.3, "dGKIN_H2O": 10.8, "ddGKIN_H2O": -5.23, "PhiF_H2O": 0.37, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "SEC_STR": "Helix", "RSA": "26.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.0, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 2.64, "dln(ku)_H2O": 1.3, "dGKIN_H2O": 10.8, "ddGKIN_H2O": -5.23, "PhiF_H2O": 0.37, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "28.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.96, "dln(kf)_H2O": 0.15, "ln(ku)_H2O": 1.16, "dln(ku)_H2O": -0.18, "dGKIN_H2O": 16.85, "ddGKIN_H2O": 0.82, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "28.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.96, "dln(kf)_H2O": 0.15, "ln(ku)_H2O": 1.16, "dln(ku)_H2O": -0.18, "dGKIN_H2O": 16.85, "ddGKIN_H2O": 0.82, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A812G", "MUTATION_PDB": "A26G", "SEC_STR": "Coil", "RSA": "42.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.19, "dln(kf)_H2O": -0.62, "ln(ku)_H2O": 1.39, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 14.37, "ddGKIN_H2O": -1.66, "PhiF_H2O": 0.91, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A812G", "MUTATION_PDB": "A26G", "SEC_STR": "Coil", "RSA": "42.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.19, "dln(kf)_H2O": -0.62, "ln(ku)_H2O": 1.39, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 14.37, "ddGKIN_H2O": -1.66, "PhiF_H2O": 0.91, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "36.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.17, "dln(kf)_H2O": -1.64, "ln(ku)_H2O": 2.48, "dln(ku)_H2O": 1.14, "dGKIN_H2O": 9.14, "ddGKIN_H2O": -6.89, "PhiF_H2O": 0.59, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "36.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.17, "dln(kf)_H2O": -1.64, "ln(ku)_H2O": 2.48, "dln(ku)_H2O": 1.14, "dGKIN_H2O": 9.14, "ddGKIN_H2O": -6.89, "PhiF_H2O": 0.59, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "11.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.02, "dln(kf)_H2O": -0.79, "ln(ku)_H2O": 1.34, "dGKIN_H2O": 14.07, "ddGKIN_H2O": -1.96, "PhiF_H2O": 0.98, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "11.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.02, "dln(kf)_H2O": -0.79, "ln(ku)_H2O": 1.34, "dGKIN_H2O": 14.07, "ddGKIN_H2O": -1.96, "PhiF_H2O": 0.98, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L818A", "MUTATION_PDB": "L32A", "SEC_STR": "Helix", "RSA": "29.3", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.09, "dln(kf)_H2O": -0.72, "ln(ku)_H2O": 2.4, "dln(ku)_H2O": 1.06, "dGKIN_H2O": 11.62, "ddGKIN_H2O": -4.41, "PhiF_H2O": 0.41, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L818A", "MUTATION_PDB": "L32A", "SEC_STR": "Helix", "RSA": "29.3", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.09, "dln(kf)_H2O": -0.72, "ln(ku)_H2O": 2.4, "dln(ku)_H2O": 1.06, "dGKIN_H2O": 11.62, "ddGKIN_H2O": -4.41, "PhiF_H2O": 0.41, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A820G", "MUTATION_PDB": "A34G", "SEC_STR": "Helix", "RSA": "37.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.9, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 1.06, "dln(ku)_H2O": -0.28, "dGKIN_H2O": 14.47, "ddGKIN_H2O": -1.56, "PhiF_H2O": 1.4, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A820G", "MUTATION_PDB": "A34G", "SEC_STR": "Helix", "RSA": "37.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.9, "dln(kf)_H2O": -0.91, "ln(ku)_H2O": 1.06, "dln(ku)_H2O": -0.28, "dGKIN_H2O": 14.47, "ddGKIN_H2O": -1.56, "PhiF_H2O": 1.4, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "6.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.0, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 3.04, "dln(ku)_H2O": 1.7, "dGKIN_H2O": 9.81, "ddGKIN_H2O": -6.22, "PhiF_H2O": 0.32, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "6.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.0, "dln(kf)_H2O": -0.81, "ln(ku)_H2O": 3.04, "dln(ku)_H2O": 1.7, "dGKIN_H2O": 9.81, "ddGKIN_H2O": -6.22, "PhiF_H2O": 0.32, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "58.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.74, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 2.3, "dln(ku)_H2O": 0.96, "dGKIN_H2O": 13.48, "ddGKIN_H2O": -2.55, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "58.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.74, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 2.3, "dln(ku)_H2O": 0.96, "dGKIN_H2O": 13.48, "ddGKIN_H2O": -2.55, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Helix", "RSA": "17.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.94, "dln(kf)_H2O": 0.13, "ln(ku)_H2O": 3.3, "dln(ku)_H2O": 1.96, "dGKIN_H2O": 11.5, "ddGKIN_H2O": -4.53, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Helix", "RSA": "17.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.94, "dln(kf)_H2O": 0.13, "ln(ku)_H2O": 3.3, "dln(ku)_H2O": 1.96, "dGKIN_H2O": 11.5, "ddGKIN_H2O": -4.53, "PhiF_H2O": 0.07, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.38, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 4.78, "dln(ku)_H2O": 3.44, "dGKIN_H2O": 6.44, "ddGKIN_H2O": -9.59, "PhiF_H2O": 0.11, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.38, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 4.78, "dln(ku)_H2O": 3.44, "dGKIN_H2O": 6.44, "ddGKIN_H2O": -9.59, "PhiF_H2O": 0.11, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T830S", "MUTATION_PDB": "T44S", "SEC_STR": "Helix", "RSA": "50.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.01, "dln(kf)_H2O": 0.2, "ln(ku)_H2O": 1.13, "dln(ku)_H2O": -0.21, "dGKIN_H2O": 17.05, "ddGKIN_H2O": 1.02, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T830S", "MUTATION_PDB": "T44S", "SEC_STR": "Helix", "RSA": "50.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.01, "dln(kf)_H2O": 0.2, "ln(ku)_H2O": 1.13, "dln(ku)_H2O": -0.21, "dGKIN_H2O": 17.05, "ddGKIN_H2O": 1.02, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.22, "dln(kf)_H2O": -0.59, "ln(ku)_H2O": 2.71, "dln(ku)_H2O": 1.37, "dGKIN_H2O": 11.17, "ddGKIN_H2O": -4.86, "PhiF_H2O": 0.3, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga77 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.22, "dln(kf)_H2O": -0.59, "ln(ku)_H2O": 2.71, "dln(ku)_H2O": 1.37, "dGKIN_H2O": 11.17, "ddGKIN_H2O": -4.86, "PhiF_H2O": 0.3, "BACKGROUND_MUT_PDB": [ "T51K", "F52A" ], "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.31, "ln(ku)_H2O": 1.86, "dGKIN_H2O": 13.5, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.31, "ln(ku)_H2O": 1.86, "dGKIN_H2O": 13.5, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A798G", "MUTATION_PDB": "A12G", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.52, "dln(kf)_H2O": 1.21, "ln(ku)_H2O": 6.91, "dln(ku)_H2O": 5.05, "dGKIN_H2O": 3.99, "ddGKIN_H2O": -9.51, "PhiF_H2O": 0.32, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A798G", "MUTATION_PDB": "A12G", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.52, "dln(kf)_H2O": 1.21, "ln(ku)_H2O": 6.91, "dln(ku)_H2O": 5.05, "dGKIN_H2O": 3.99, "ddGKIN_H2O": -9.51, "PhiF_H2O": 0.32, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "23.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.55, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 2.04, "dln(ku)_H2O": 0.18, "dGKIN_H2O": 11.17, "ddGKIN_H2O": -2.33, "PhiF_H2O": 0.81, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I803V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "23.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.55, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 2.04, "dln(ku)_H2O": 0.18, "dGKIN_H2O": 11.17, "ddGKIN_H2O": -2.33, "PhiF_H2O": 0.81, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "1.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.51, "dln(kf)_H2O": -0.8, "ln(ku)_H2O": 5.63, "dln(ku)_H2O": 3.77, "dGKIN_H2O": 2.18, "ddGKIN_H2O": -11.32, "PhiF_H2O": 0.18, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L806A", "MUTATION_PDB": "L20A", "SEC_STR": "Helix", "RSA": "1.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.51, "dln(kf)_H2O": -0.8, "ln(ku)_H2O": 5.63, "dln(ku)_H2O": 3.77, "dGKIN_H2O": 2.18, "ddGKIN_H2O": -11.32, "PhiF_H2O": 0.18, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "SEC_STR": "Helix", "RSA": "26.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.65, "dln(kf)_H2O": -0.66, "ln(ku)_H2O": 3.61, "dln(ku)_H2O": 1.75, "dGKIN_H2O": 7.53, "ddGKIN_H2O": -5.97, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A809G", "MUTATION_PDB": "A23G", "SEC_STR": "Helix", "RSA": "26.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.65, "dln(kf)_H2O": -0.66, "ln(ku)_H2O": 3.61, "dln(ku)_H2O": 1.75, "dGKIN_H2O": 7.53, "ddGKIN_H2O": -5.97, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "28.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.31, "ln(ku)_H2O": 2.03, "dln(ku)_H2O": 0.17, "dGKIN_H2O": 13.08, "ddGKIN_H2O": -0.42, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I811V", "MUTATION_PDB": "I25V", "SEC_STR": "Coil", "RSA": "28.4", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.31, "ln(ku)_H2O": 2.03, "dln(ku)_H2O": 0.17, "dGKIN_H2O": 13.08, "ddGKIN_H2O": -0.42, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A812G", "MUTATION_PDB": "A26G", "SEC_STR": "Coil", "RSA": "42.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.63, "dln(kf)_H2O": -0.68, "ln(ku)_H2O": 2.3, "dln(ku)_H2O": 0.44, "dGKIN_H2O": 10.73, "ddGKIN_H2O": -2.77, "PhiF_H2O": 0.61, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A812G", "MUTATION_PDB": "A26G", "SEC_STR": "Coil", "RSA": "42.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.63, "dln(kf)_H2O": -0.68, "ln(ku)_H2O": 2.3, "dln(ku)_H2O": 0.44, "dGKIN_H2O": 10.73, "ddGKIN_H2O": -2.77, "PhiF_H2O": 0.61, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "36.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 5.35, "dln(kf)_H2O": -1.96, "ln(ku)_H2O": 3.47, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 4.66, "ddGKIN_H2O": -8.84, "PhiF_H2O": 0.55, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "Y815A", "MUTATION_PDB": "Y29A", "SEC_STR": "Helix", "RSA": "36.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 5.35, "dln(kf)_H2O": -1.96, "ln(ku)_H2O": 3.47, "dln(ku)_H2O": 1.61, "dGKIN_H2O": 4.66, "ddGKIN_H2O": -8.84, "PhiF_H2O": 0.55, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "11.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.21, "dln(kf)_H2O": -1.1, "ln(ku)_H2O": 2.24, "dln(ku)_H2O": 0.38, "dGKIN_H2O": 9.84, "ddGKIN_H2O": -3.66, "PhiF_H2O": 0.74, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I816V", "MUTATION_PDB": "I30V", "SEC_STR": "Helix", "RSA": "11.8", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.21, "dln(kf)_H2O": -1.1, "ln(ku)_H2O": 2.24, "dln(ku)_H2O": 0.38, "dGKIN_H2O": 9.84, "ddGKIN_H2O": -3.66, "PhiF_H2O": 0.74, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A820G", "MUTATION_PDB": "A34G", "SEC_STR": "Helix", "RSA": "37.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.02, "dln(kf)_H2O": -1.29, "ln(ku)_H2O": 1.81, "dln(ku)_H2O": -0.05, "dGKIN_H2O": 10.43, "ddGKIN_H2O": -3.07, "PhiF_H2O": 1.04, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A820G", "MUTATION_PDB": "A34G", "SEC_STR": "Helix", "RSA": "37.7", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.02, "dln(kf)_H2O": -1.29, "ln(ku)_H2O": 1.81, "dln(ku)_H2O": -0.05, "dGKIN_H2O": 10.43, "ddGKIN_H2O": -3.07, "PhiF_H2O": 1.04, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "6.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 5.3, "dln(kf)_H2O": -2.01, "ln(ku)_H2O": 3.74, "dln(ku)_H2O": 1.88, "dGKIN_H2O": 3.87, "ddGKIN_H2O": -9.63, "PhiF_H2O": 0.52, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "A822G", "MUTATION_PDB": "A36G", "SEC_STR": "Coil", "RSA": "6.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 5.3, "dln(kf)_H2O": -2.01, "ln(ku)_H2O": 3.74, "dln(ku)_H2O": 1.88, "dGKIN_H2O": 3.87, "ddGKIN_H2O": -9.63, "PhiF_H2O": 0.52, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "58.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.09, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 2.77, "dln(ku)_H2O": 0.91, "dGKIN_H2O": 10.7, "ddGKIN_H2O": -2.8, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T824S", "MUTATION_PDB": "T38S", "SEC_STR": "Coil", "RSA": "58.5", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.09, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 2.77, "dln(ku)_H2O": 0.91, "dGKIN_H2O": 10.7, "ddGKIN_H2O": -2.8, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Helix", "RSA": "17.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.86, "dln(kf)_H2O": -0.45, "ln(ku)_H2O": 3.66, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 7.93, "ddGKIN_H2O": -5.57, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V825A", "MUTATION_PDB": "V39A", "SEC_STR": "Helix", "RSA": "17.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.86, "dln(kf)_H2O": -0.45, "ln(ku)_H2O": 3.66, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 7.93, "ddGKIN_H2O": -5.57, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.41, "dln(kf)_H2O": -0.9, "ln(ku)_H2O": 5.56, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 2.11, "ddGKIN_H2O": -11.39, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "V828A", "MUTATION_PDB": "V42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.41, "dln(kf)_H2O": -0.9, "ln(ku)_H2O": 5.56, "dln(ku)_H2O": 3.7, "dGKIN_H2O": 2.11, "ddGKIN_H2O": -11.39, "PhiF_H2O": 0.2, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T830S", "MUTATION_PDB": "T44S", "SEC_STR": "Helix", "RSA": "50.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.48, "dln(kf)_H2O": 0.17, "ln(ku)_H2O": 1.96, "dln(ku)_H2O": 0.1, "dGKIN_H2O": 13.68, "ddGKIN_H2O": 0.18, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T830S", "MUTATION_PDB": "T44S", "SEC_STR": "Helix", "RSA": "50.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.48, "dln(kf)_H2O": 0.17, "ln(ku)_H2O": 1.96, "dln(ku)_H2O": 0.1, "dGKIN_H2O": 13.68, "ddGKIN_H2O": 0.18, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.57, "dln(kf)_H2O": 0.26, "ln(ku)_H2O": 4.14, "dln(ku)_H2O": 2.28, "dGKIN_H2O": 8.5, "ddGKIN_H2O": -5.0, "PhiF_H2O": 0.07, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L831A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "25.6", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.57, "dln(kf)_H2O": 0.26, "ln(ku)_H2O": 4.14, "dln(ku)_H2O": 2.28, "dGKIN_H2O": 8.5, "ddGKIN_H2O": -5.0, "PhiF_H2O": 0.07, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I835A", "MUTATION_PDB": "I49A", "SEC_STR": "Helix", "RSA": "1.2", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.1, "dln(kf)_H2O": -0.21, "ln(ku)_H2O": 3.93, "dln(ku)_H2O": 2.07, "dGKIN_H2O": 7.85, "ddGKIN_H2O": -5.65, "PhiF_H2O": 0.09, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I835A", "MUTATION_PDB": "I49A", "SEC_STR": "Helix", "RSA": "1.2", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.1, "dln(kf)_H2O": -0.21, "ln(ku)_H2O": 3.93, "dln(ku)_H2O": 2.07, "dGKIN_H2O": 7.85, "ddGKIN_H2O": -5.65, "PhiF_H2O": 0.09, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L836A", "MUTATION_PDB": "L50A", "SEC_STR": "Helix", "RSA": "28.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.59, "dln(kf)_H2O": 0.28, "ln(ku)_H2O": 4.49, "dln(ku)_H2O": 2.63, "dGKIN_H2O": 7.68, "ddGKIN_H2O": -5.82, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "L836A", "MUTATION_PDB": "L50A", "SEC_STR": "Helix", "RSA": "28.0", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.59, "dln(kf)_H2O": 0.28, "ln(ku)_H2O": 4.49, "dln(ku)_H2O": 2.63, "dGKIN_H2O": 7.68, "ddGKIN_H2O": -5.82, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T837S", "MUTATION_PDB": "T51S", "SEC_STR": "Helix", "RSA": "73.9", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.02, "dln(kf)_H2O": 0.71, "ln(ku)_H2O": 1.46, "dln(ku)_H2O": -0.4, "dGKIN_H2O": 16.25, "ddGKIN_H2O": 2.75, "PhiF_H2O": 0.64, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "PSD1(Ga88 mutant)", "SOURCE": "Finegoldia magna", "LENGTH": 50.0, "PDB_wild": "2jws", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "T837S", "MUTATION_PDB": "T51S", "SEC_STR": "Helix", "RSA": "73.9", "T": 10.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow, or T-Jump Fluorescence Sectroscopy", "METHOD_KIN": "urea", "ln(kf)_H2O": 8.02, "dln(kf)_H2O": 0.71, "ln(ku)_H2O": 1.46, "dln(ku)_H2O": -0.4, "dGKIN_H2O": 16.25, "ddGKIN_H2O": 2.75, "PhiF_H2O": 0.64, "AUTHOR_KIN": "Giri R, Morrone A, Travaglini-Allocatelli C, Jemth P, Brunori M, Gianni S.", "REFERENCE_KIN": "Folding pathways of proteins with increasing degree of sequence identities but different structure and function. Proc Natl Acad Sci U S A 109(44):17772-6. https://doi.org/10.1073/pnas.1201794109", "YEAR_KIN": 2012.0, "PMID_KIN": "22652570", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y449F", "MUTATION_PDB": "Y20F", "SEC_STR": "Beta", "RSA": "10.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.99, "dln(kf)_H2O": -0.06, "ln(ku)_H2O": 6.98, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 7.03, "ddGKIN_H2O": -2.85, "mf": -2.89, "mu": 1.47, "PhiF_H2O": 0.05, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.076", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y449F", "MUTATION_PDB": "Y20F", "SEC_STR": "Beta", "RSA": "10.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.99, "dln(kf)_H2O": -0.06, "ln(ku)_H2O": 6.98, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 7.03, "ddGKIN_H2O": -2.85, "mf": -2.89, "mu": 1.47, "PhiF_H2O": 0.05, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.076", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y450A", "MUTATION_PDB": "Y21A", "SEC_STR": "Beta", "RSA": "26.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.2, "dln(kf)_H2O": -0.85, "ln(ku)_H2O": 8.01, "dln(ku)_H2O": 2.18, "dGKIN_H2O": 2.8, "ddGKIN_H2O": -7.11, "mf": -4.6, "mu": 2.13, "PhiF_H2O": 0.28, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.077", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y450A", "MUTATION_PDB": "Y21A", "SEC_STR": "Beta", "RSA": "26.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.2, "dln(kf)_H2O": -0.85, "ln(ku)_H2O": 8.01, "dln(ku)_H2O": 2.18, "dGKIN_H2O": 2.8, "ddGKIN_H2O": -7.11, "mf": -4.6, "mu": 2.13, "PhiF_H2O": 0.28, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.077", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "N452A", "MUTATION_PDB": "N23A", "SEC_STR": "Coil", "RSA": "27.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.26, "dln(kf)_H2O": 0.21, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 9.67, "ddGKIN_H2O": -0.29, "mf": -2.72, "mu": 1.72, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.079", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "N452A", "MUTATION_PDB": "N23A", "SEC_STR": "Coil", "RSA": "27.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.26, "dln(kf)_H2O": 0.21, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 9.67, "ddGKIN_H2O": -0.29, "mf": -2.72, "mu": 1.72, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.079", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "R453A", "MUTATION_PDB": "R24A", "SEC_STR": "Coil", "RSA": "73.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -0.41, "ln(ku)_H2O": 6.82, "dln(ku)_H2O": 0.99, "dGKIN_H2O": 6.61, "ddGKIN_H2O": -3.26, "mf": -2.47, "mu": 1.23, "PhiF_H2O": 0.29, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.080", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "R453A", "MUTATION_PDB": "R24A", "SEC_STR": "Coil", "RSA": "73.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -0.41, "ln(ku)_H2O": 6.82, "dln(ku)_H2O": 0.99, "dGKIN_H2O": 6.61, "ddGKIN_H2O": -3.26, "mf": -2.47, "mu": 1.23, "PhiF_H2O": 0.29, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.080", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T454A", "MUTATION_PDB": "T25A", "SEC_STR": "Coil", "RSA": "54.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.32, "dln(kf)_H2O": -1.73, "ln(ku)_H2O": 8.59, "dln(ku)_H2O": 2.76, "ddGKIN_H2O": -10.5, "mf": -5.56, "mu": 2.05, "PhiF_H2O": 0.39, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.081", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T454A", "MUTATION_PDB": "T25A", "SEC_STR": "Coil", "RSA": "54.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.32, "dln(kf)_H2O": -1.73, "ln(ku)_H2O": 8.59, "dln(ku)_H2O": 2.76, "ddGKIN_H2O": -10.5, "mf": -5.56, "mu": 2.05, "PhiF_H2O": 0.39, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.081", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T454S", "MUTATION_PDB": "T25S", "SEC_STR": "Coil", "RSA": "54.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -0.51, "ln(ku)_H2O": 7.24, "dln(ku)_H2O": 1.41, "dGKIN_H2O": 5.4, "ddGKIN_H2O": -4.52, "mf": -3.05, "mu": 2.05, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.082", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T454S", "MUTATION_PDB": "T25S", "SEC_STR": "Coil", "RSA": "54.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.54, "dln(kf)_H2O": -0.51, "ln(ku)_H2O": 7.24, "dln(ku)_H2O": 1.41, "dGKIN_H2O": 5.4, "ddGKIN_H2O": -4.52, "mf": -3.05, "mu": 2.05, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.082", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.05, "ln(ku)_H2O": 5.83, "dGKIN_H2O": 9.92, "mf": -2.43, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.050", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.05, "ln(ku)_H2O": 5.83, "dGKIN_H2O": 9.92, "mf": -2.43, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.050", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T432A", "MUTATION_PDB": "T3A", "SEC_STR": "Coil", "RSA": "62.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.95, "dln(kf)_H2O": -0.1, "ln(ku)_H2O": 5.89, "dln(ku)_H2O": 0.06, "dGKIN_H2O": 9.54, "ddGKIN_H2O": -0.38, "mf": -2.51, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.051", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T432A", "MUTATION_PDB": "T3A", "SEC_STR": "Coil", "RSA": "62.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.95, "dln(kf)_H2O": -0.1, "ln(ku)_H2O": 5.89, "dln(ku)_H2O": 0.06, "dGKIN_H2O": 9.54, "ddGKIN_H2O": -0.38, "mf": -2.51, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.051", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "A433G", "MUTATION_PDB": "A4G", "SEC_STR": "Coil", "RSA": "63.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.38, "dln(kf)_H2O": 0.33, "ln(ku)_H2O": 6.45, "dln(ku)_H2O": 0.62, "dGKIN_H2O": 9.2, "ddGKIN_H2O": -0.67, "mf": -3.05, "mu": 1.47, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.052", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "A433G", "MUTATION_PDB": "A4G", "SEC_STR": "Coil", "RSA": "63.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.38, "dln(kf)_H2O": 0.33, "ln(ku)_H2O": 6.45, "dln(ku)_H2O": 0.62, "dGKIN_H2O": 9.2, "ddGKIN_H2O": -0.67, "mf": -3.05, "mu": 1.47, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.052", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "V434A", "MUTATION_PDB": "V5A", "SEC_STR": "Coil", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.36, "dln(kf)_H2O": 0.31, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 0.32, "dGKIN_H2O": 9.83, "ddGKIN_H2O": -0.04, "mf": -2.89, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.053", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "V434A", "MUTATION_PDB": "V5A", "SEC_STR": "Coil", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.36, "dln(kf)_H2O": 0.31, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 0.32, "dGKIN_H2O": 9.83, "ddGKIN_H2O": -0.04, "mf": -2.89, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.053", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "V434G", "MUTATION_PDB": "V5G", "SEC_STR": "Coil", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.28, "dln(kf)_H2O": 0.23, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": 0.65, "dGKIN_H2O": 8.91, "ddGKIN_H2O": -1.0, "mf": -2.8, "mu": 1.39, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.054", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "V434G", "MUTATION_PDB": "V5G", "SEC_STR": "Coil", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.28, "dln(kf)_H2O": 0.23, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": 0.65, "dGKIN_H2O": 8.91, "ddGKIN_H2O": -1.0, "mf": -2.8, "mu": 1.39, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.054", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S435A", "MUTATION_PDB": "S6A", "SEC_STR": "Coil", "RSA": "59.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.68, "dln(kf)_H2O": -0.37, "ln(ku)_H2O": 5.86, "dln(ku)_H2O": 0.03, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -0.92, "mf": -2.3, "mu": 2.37, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.055", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S435A", "MUTATION_PDB": "S6A", "SEC_STR": "Coil", "RSA": "59.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.68, "dln(kf)_H2O": -0.37, "ln(ku)_H2O": 5.86, "dln(ku)_H2O": 0.03, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -0.92, "mf": -2.3, "mu": 2.37, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.055", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438S", "MUTATION_PDB": "T9S", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.59, "dln(kf)_H2O": -0.46, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": -0.39, "dGKIN_H2O": 9.71, "ddGKIN_H2O": -0.17, "mf": -2.3, "mu": 2.37, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.060", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438S", "MUTATION_PDB": "T9S", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.59, "dln(kf)_H2O": -0.46, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": -0.39, "dGKIN_H2O": 9.71, "ddGKIN_H2O": -0.17, "mf": -2.3, "mu": 2.37, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.060", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E439A", "MUTATION_PDB": "E10A", "SEC_STR": "Beta", "RSA": "55.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.76, "dln(kf)_H2O": 0.71, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": 0.65, "dGKIN_H2O": 10.04, "ddGKIN_H2O": 0.63, "mf": -2.93, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.061", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E439A", "MUTATION_PDB": "E10A", "SEC_STR": "Beta", "RSA": "55.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.76, "dln(kf)_H2O": 0.71, "ln(ku)_H2O": 6.48, "dln(ku)_H2O": 0.65, "dGKIN_H2O": 10.04, "ddGKIN_H2O": 0.63, "mf": -2.93, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.061", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y440A", "MUTATION_PDB": "Y11A", "SEC_STR": "Beta", "RSA": "53.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 0.52, "dGKIN_H2O": 7.28, "ddGKIN_H2O": -2.64, "mf": -2.22, "mu": 2.21, "PhiF_H2O": 0.55, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.062", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y440A", "MUTATION_PDB": "Y11A", "SEC_STR": "Beta", "RSA": "53.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 0.52, "dGKIN_H2O": 7.28, "ddGKIN_H2O": -2.64, "mf": -2.22, "mu": 2.21, "PhiF_H2O": 0.55, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.062", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y440F", "MUTATION_PDB": "Y11F", "SEC_STR": "Beta", "RSA": "53.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.76, "dln(kf)_H2O": -0.29, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 0.32, "dGKIN_H2O": 8.45, "ddGKIN_H2O": -1.42, "mf": -2.55, "mu": 1.8, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.063", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y440F", "MUTATION_PDB": "Y11F", "SEC_STR": "Beta", "RSA": "53.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.76, "dln(kf)_H2O": -0.29, "ln(ku)_H2O": 6.15, "dln(ku)_H2O": 0.32, "dGKIN_H2O": 8.45, "ddGKIN_H2O": -1.42, "mf": -2.55, "mu": 1.8, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.063", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442A", "MUTATION_PDB": "T13A", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.1, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 7.32, "dln(ku)_H2O": 1.49, "dGKIN_H2O": 6.53, "ddGKIN_H2O": -3.39, "mf": -2.97, "mu": 1.47, "PhiF_H2O": -0.03, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.064", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442A", "MUTATION_PDB": "T13A", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.1, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 7.32, "dln(ku)_H2O": 1.49, "dGKIN_H2O": 6.53, "ddGKIN_H2O": -3.39, "mf": -2.97, "mu": 1.47, "PhiF_H2O": -0.03, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.064", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442G", "MUTATION_PDB": "T13G", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.39, "dln(kf)_H2O": 0.34, "ln(ku)_H2O": 7.21, "dln(ku)_H2O": 1.38, "dGKIN_H2O": 7.45, "ddGKIN_H2O": -2.43, "mf": -2.51, "mu": 1.8, "PhiF_H2O": -0.32, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.065", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442G", "MUTATION_PDB": "T13G", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.39, "dln(kf)_H2O": 0.34, "ln(ku)_H2O": 7.21, "dln(ku)_H2O": 1.38, "dGKIN_H2O": 7.45, "ddGKIN_H2O": -2.43, "mf": -2.51, "mu": 1.8, "PhiF_H2O": -0.32, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.065", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442S", "MUTATION_PDB": "T13S", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.01, "dln(kf)_H2O": -0.04, "ln(ku)_H2O": 6.19, "dln(ku)_H2O": 0.36, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -0.96, "mf": -2.59, "mu": 2.21, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.066", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T442S", "MUTATION_PDB": "T13S", "SEC_STR": "Coil", "RSA": "26.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.01, "dln(kf)_H2O": -0.04, "ln(ku)_H2O": 6.19, "dln(ku)_H2O": 0.36, "dGKIN_H2O": 8.95, "ddGKIN_H2O": -0.96, "mf": -2.59, "mu": 2.21, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.066", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "A443G", "MUTATION_PDB": "A14G", "SEC_STR": "Coil", "RSA": "100.0", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 6.11, "dln(ku)_H2O": 0.28, "dGKIN_H2O": 7.78, "ddGKIN_H2O": -2.09, "mf": -3.43, "mu": 1.64, "PhiF_H2O": 0.69, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.067", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "A443G", "MUTATION_PDB": "A14G", "SEC_STR": "Coil", "RSA": "100.0", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 6.11, "dln(ku)_H2O": 0.28, "dGKIN_H2O": 7.78, "ddGKIN_H2O": -2.09, "mf": -3.43, "mu": 1.64, "PhiF_H2O": 0.69, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.067", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "D444A", "MUTATION_PDB": "D15A", "SEC_STR": "Coil", "RSA": "95.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 5.97, "dln(ku)_H2O": 0.14, "dGKIN_H2O": 8.12, "ddGKIN_H2O": -1.76, "mf": -3.01, "mu": 1.8, "PhiF_H2O": 0.82, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.068", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "D444A", "MUTATION_PDB": "D15A", "SEC_STR": "Coil", "RSA": "95.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.44, "dln(kf)_H2O": -0.61, "ln(ku)_H2O": 5.97, "dln(ku)_H2O": 0.14, "dGKIN_H2O": 8.12, "ddGKIN_H2O": -1.76, "mf": -3.01, "mu": 1.8, "PhiF_H2O": 0.82, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.068", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "D444G", "MUTATION_PDB": "D15G", "SEC_STR": "Coil", "RSA": "95.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.52, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 5.99, "dln(ku)_H2O": 0.16, "dGKIN_H2O": 8.24, "ddGKIN_H2O": -1.63, "mf": -2.59, "mu": 2.05, "PhiF_H2O": 0.77, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.069", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "D444G", "MUTATION_PDB": "D15G", "SEC_STR": "Coil", "RSA": "95.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.52, "dln(kf)_H2O": -0.53, "ln(ku)_H2O": 5.99, "dln(ku)_H2O": 0.16, "dGKIN_H2O": 8.24, "ddGKIN_H2O": -1.63, "mf": -2.59, "mu": 2.05, "PhiF_H2O": 0.77, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.069", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L465V", "MUTATION_PDB": "L36V", "SEC_STR": "Coil", "RSA": "14.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.17, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 6.91, "dln(ku)_H2O": 1.08, "dGKIN_H2O": 7.66, "ddGKIN_H2O": -2.22, "mf": -2.97, "mu": 1.31, "PhiF_H2O": -0.13, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.098", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L465V", "MUTATION_PDB": "L36V", "SEC_STR": "Coil", "RSA": "14.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.17, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 6.91, "dln(ku)_H2O": 1.08, "dGKIN_H2O": 7.66, "ddGKIN_H2O": -2.22, "mf": -2.97, "mu": 1.31, "PhiF_H2O": -0.13, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.098", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L455A", "MUTATION_PDB": "L26A", "SEC_STR": "Coil", "RSA": "73.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.98, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 6.75, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 7.53, "ddGKIN_H2O": -2.34, "mf": -2.55, "mu": 0.9, "PhiF_H2O": 0.08, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.083", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L455A", "MUTATION_PDB": "L26A", "SEC_STR": "Coil", "RSA": "73.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.98, "dln(kf)_H2O": -0.07, "ln(ku)_H2O": 6.75, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 7.53, "ddGKIN_H2O": -2.34, "mf": -2.55, "mu": 0.9, "PhiF_H2O": 0.08, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.083", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L455G", "MUTATION_PDB": "L26G", "SEC_STR": "Coil", "RSA": "73.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 11.09, "dln(kf)_H2O": 1.04, "ln(ku)_H2O": 4.55, "dln(ku)_H2O": -1.28, "dGKIN_H2O": 15.31, "ddGKIN_H2O": 5.4, "mf": -1.8, "mu": 1.96, "PhiF_H2O": 0.45, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.084", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L455G", "MUTATION_PDB": "L26G", "SEC_STR": "Coil", "RSA": "73.2", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 11.09, "dln(kf)_H2O": 1.04, "ln(ku)_H2O": 4.55, "dln(ku)_H2O": -1.28, "dGKIN_H2O": 15.31, "ddGKIN_H2O": 5.4, "mf": -1.8, "mu": 1.96, "PhiF_H2O": 0.45, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.084", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E456A", "MUTATION_PDB": "E27A", "SEC_STR": "Beta", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.83, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 7.43, "dln(ku)_H2O": 1.6, "dGKIN_H2O": 5.65, "ddGKIN_H2O": -4.27, "mf": -2.38, "mu": 1.88, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.085", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E456A", "MUTATION_PDB": "E27A", "SEC_STR": "Beta", "RSA": "61.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.83, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 7.43, "dln(ku)_H2O": 1.6, "dGKIN_H2O": 5.65, "ddGKIN_H2O": -4.27, "mf": -2.38, "mu": 1.88, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.085", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S457A", "MUTATION_PDB": "S28A", "SEC_STR": "Beta", "RSA": "50.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.53, "dln(kf)_H2O": 0.48, "ln(ku)_H2O": 6.29, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 9.92, "ddGKIN_H2O": 0.04, "mf": -2.89, "mu": 2.05, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.086", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S457A", "MUTATION_PDB": "S28A", "SEC_STR": "Beta", "RSA": "50.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.53, "dln(kf)_H2O": 0.48, "ln(ku)_H2O": 6.29, "dln(ku)_H2O": 0.46, "dGKIN_H2O": 9.92, "ddGKIN_H2O": 0.04, "mf": -2.89, "mu": 2.05, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.086", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S457G", "MUTATION_PDB": "S28G", "SEC_STR": "Beta", "RSA": "50.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.15, "dln(kf)_H2O": 0.1, "ln(ku)_H2O": 6.25, "dln(ku)_H2O": 0.42, "dGKIN_H2O": 9.12, "ddGKIN_H2O": -0.79, "mf": -2.76, "mu": 2.54, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.087", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "S457G", "MUTATION_PDB": "S28G", "SEC_STR": "Beta", "RSA": "50.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.15, "dln(kf)_H2O": 0.1, "ln(ku)_H2O": 6.25, "dln(ku)_H2O": 0.42, "dGKIN_H2O": 9.12, "ddGKIN_H2O": -0.79, "mf": -2.76, "mu": 2.54, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.087", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458A", "MUTATION_PDB": "T29A", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.57, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 5.94, "dln(ku)_H2O": 0.11, "dGKIN_H2O": 8.54, "ddGKIN_H2O": -1.38, "mf": -2.89, "mu": 1.8, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.088", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458A", "MUTATION_PDB": "T29A", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.57, "dln(kf)_H2O": -0.48, "ln(ku)_H2O": 5.94, "dln(ku)_H2O": 0.11, "dGKIN_H2O": 8.54, "ddGKIN_H2O": -1.38, "mf": -2.89, "mu": 1.8, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.088", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458G", "MUTATION_PDB": "T29G", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.75, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 8.9, "dln(ku)_H2O": 3.07, "dGKIN_H2O": 1.97, "ddGKIN_H2O": -7.91, "mf": -3.64, "mu": 1.96, "PhiF_H2O": 0.09, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.089", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458G", "MUTATION_PDB": "T29G", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.75, "dln(kf)_H2O": -0.3, "ln(ku)_H2O": 8.9, "dln(ku)_H2O": 3.07, "dGKIN_H2O": 1.97, "ddGKIN_H2O": -7.91, "mf": -3.64, "mu": 1.96, "PhiF_H2O": 0.09, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.089", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458S", "MUTATION_PDB": "T29S", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.16, "dln(kf)_H2O": 0.11, "ln(ku)_H2O": 6.68, "dln(ku)_H2O": 0.85, "dGKIN_H2O": 8.12, "ddGKIN_H2O": -1.76, "mf": -2.68, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.090", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T458S", "MUTATION_PDB": "T29S", "SEC_STR": "Beta", "RSA": "30.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.16, "dln(kf)_H2O": 0.11, "ln(ku)_H2O": 6.68, "dln(ku)_H2O": 0.85, "dGKIN_H2O": 8.12, "ddGKIN_H2O": -1.76, "mf": -2.68, "mu": 1.64, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.090", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "W459A", "MUTATION_PDB": "W30A", "SEC_STR": "Coil", "RSA": "64.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.8, "dln(kf)_H2O": -0.25, "ln(ku)_H2O": 6.94, "dln(ku)_H2O": 1.11, "dGKIN_H2O": 6.69, "ddGKIN_H2O": -3.18, "mf": -2.43, "mu": 1.88, "PhiF_H2O": 0.19, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.091", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "W459A", "MUTATION_PDB": "W30A", "SEC_STR": "Coil", "RSA": "64.8", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.8, "dln(kf)_H2O": -0.25, "ln(ku)_H2O": 6.94, "dln(ku)_H2O": 1.11, "dGKIN_H2O": 6.69, "ddGKIN_H2O": -3.18, "mf": -2.43, "mu": 1.88, "PhiF_H2O": 0.19, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.091", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E460T", "MUTATION_PDB": "E31T", "SEC_STR": "Coil", "RSA": "77.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.21, "dln(kf)_H2O": 0.16, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 9.54, "ddGKIN_H2O": -0.33, "mf": -2.72, "mu": 1.72, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.093", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E460T", "MUTATION_PDB": "E31T", "SEC_STR": "Coil", "RSA": "77.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.21, "dln(kf)_H2O": 0.16, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 9.54, "ddGKIN_H2O": -0.33, "mf": -2.72, "mu": 1.72, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.093", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "K461R", "MUTATION_PDB": "K32R", "SEC_STR": "Coil", "RSA": "28.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.35, "dln(kf)_H2O": 0.3, "ln(ku)_H2O": 6.31, "dln(ku)_H2O": 0.48, "dGKIN_H2O": 9.5, "ddGKIN_H2O": -0.42, "mf": -3.01, "mu": 1.88, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.094", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "K461R", "MUTATION_PDB": "K32R", "SEC_STR": "Coil", "RSA": "28.3", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.35, "dln(kf)_H2O": 0.3, "ln(ku)_H2O": 6.31, "dln(ku)_H2O": 0.48, "dGKIN_H2O": 9.5, "ddGKIN_H2O": -0.42, "mf": -3.01, "mu": 1.88, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.094", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E464A", "MUTATION_PDB": "E35A", "SEC_STR": "Coil", "RSA": "21.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 0.52, "dGKIN_H2O": 8.79, "ddGKIN_H2O": -1.13, "mf": -2.76, "mu": 1.31, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.096", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E464A", "MUTATION_PDB": "E35A", "SEC_STR": "Coil", "RSA": "21.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.07, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 6.35, "dln(ku)_H2O": 0.52, "dGKIN_H2O": 8.79, "ddGKIN_H2O": -1.13, "mf": -2.76, "mu": 1.31, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.096", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L465A", "MUTATION_PDB": "L36A", "SEC_STR": "Coil", "RSA": "14.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.54, "dln(kf)_H2O": 0.49, "ln(ku)_H2O": 7.93, "dln(ku)_H2O": 2.1, "dGKIN_H2O": 6.11, "ddGKIN_H2O": -3.81, "mf": -3.51, "mu": 1.15, "PhiF_H2O": -0.3, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.097", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "L465A", "MUTATION_PDB": "L36A", "SEC_STR": "Coil", "RSA": "14.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.54, "dln(kf)_H2O": 0.49, "ln(ku)_H2O": 7.93, "dln(ku)_H2O": 2.1, "dGKIN_H2O": 6.11, "ddGKIN_H2O": -3.81, "mf": -3.51, "mu": 1.15, "PhiF_H2O": -0.3, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.097", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "G445A", "MUTATION_PDB": "G16A", "SEC_STR": "Coil", "RSA": "66.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 7.28, "dln(kf)_H2O": -2.77, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": -0.39, "dGKIN_H2O": 5.31, "ddGKIN_H2O": -5.56, "mf": -4.81, "mu": 2.37, "PhiF_H2O": 1.17, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.070", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "G445A", "MUTATION_PDB": "G16A", "SEC_STR": "Coil", "RSA": "66.7", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 7.28, "dln(kf)_H2O": -2.77, "ln(ku)_H2O": 5.44, "dln(ku)_H2O": -0.39, "dGKIN_H2O": 5.31, "ddGKIN_H2O": -5.56, "mf": -4.81, "mu": 2.37, "PhiF_H2O": 1.17, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.070", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "K446A", "MUTATION_PDB": "K17A", "SEC_STR": "Coil", "RSA": "56.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.62, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 6.02, "dln(ku)_H2O": 0.19, "dGKIN_H2O": 8.45, "ddGKIN_H2O": -1.46, "mf": -2.55, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.071", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "K446A", "MUTATION_PDB": "K17A", "SEC_STR": "Coil", "RSA": "56.6", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.62, "dln(kf)_H2O": -0.43, "ln(ku)_H2O": 6.02, "dln(ku)_H2O": 0.19, "dGKIN_H2O": 8.45, "ddGKIN_H2O": -1.46, "mf": -2.55, "mu": 1.96, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.071", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T447A", "MUTATION_PDB": "T18A", "SEC_STR": "Beta", "RSA": "28.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.15, "dln(kf)_H2O": -0.9, "ln(ku)_H2O": 5.91, "dln(ku)_H2O": 0.08, "dGKIN_H2O": 7.61, "ddGKIN_H2O": -2.26, "mf": -3.26, "mu": 2.13, "PhiF_H2O": 0.93, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.072", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T447A", "MUTATION_PDB": "T18A", "SEC_STR": "Beta", "RSA": "28.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.15, "dln(kf)_H2O": -0.9, "ln(ku)_H2O": 5.91, "dln(ku)_H2O": 0.08, "dGKIN_H2O": 7.61, "ddGKIN_H2O": -2.26, "mf": -3.26, "mu": 2.13, "PhiF_H2O": 0.93, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.072", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T447G", "MUTATION_PDB": "T18G", "SEC_STR": "Beta", "RSA": "28.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.29, "dln(kf)_H2O": -1.76, "ln(ku)_H2O": 6.46, "dln(ku)_H2O": 0.63, "dGKIN_H2O": 4.31, "ddGKIN_H2O": -4.77, "mf": -4.6, "mu": 3.11, "PhiF_H2O": 0.73, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.073", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T447G", "MUTATION_PDB": "T18G", "SEC_STR": "Beta", "RSA": "28.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 8.29, "dln(kf)_H2O": -1.76, "ln(ku)_H2O": 6.46, "dln(ku)_H2O": 0.63, "dGKIN_H2O": 4.31, "ddGKIN_H2O": -4.77, "mf": -4.6, "mu": 3.11, "PhiF_H2O": 0.73, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.073", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y448A", "MUTATION_PDB": "Y19A", "SEC_STR": "Beta", "RSA": "23.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.28, "dln(kf)_H2O": 0.23, "ln(ku)_H2O": 7.42, "dln(ku)_H2O": 1.59, "dGKIN_H2O": 7.11, "ddGKIN_H2O": -2.8, "mf": -3.26, "mu": 2.13, "PhiF_H2O": 0.11, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.074", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y448A", "MUTATION_PDB": "Y19A", "SEC_STR": "Beta", "RSA": "23.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.28, "dln(kf)_H2O": 0.23, "ln(ku)_H2O": 7.42, "dln(ku)_H2O": 1.59, "dGKIN_H2O": 7.11, "ddGKIN_H2O": -2.8, "mf": -3.26, "mu": 2.13, "PhiF_H2O": 0.11, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.074", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y448F", "MUTATION_PDB": "Y19F", "SEC_STR": "Beta", "RSA": "23.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.12, "dln(kf)_H2O": 0.07, "ln(ku)_H2O": 5.77, "dln(ku)_H2O": -0.06, "dGKIN_H2O": 10.21, "ddGKIN_H2O": 0.29, "mf": -2.76, "mu": 2.62, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.075", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "Y448F", "MUTATION_PDB": "Y19F", "SEC_STR": "Beta", "RSA": "23.4", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.12, "dln(kf)_H2O": 0.07, "ln(ku)_H2O": 5.77, "dln(ku)_H2O": -0.06, "dGKIN_H2O": 10.21, "ddGKIN_H2O": 0.29, "mf": -2.76, "mu": 2.62, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.075", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "C-terminal \u03b2-hairpin of protein GB", "SOURCE": "Streptococcus sp. group G", "LENGTH": 16.0, "UniProt": "P06654", "PDB_wild": "1pgb", "MUTATED_CHAIN": "A", "PFAM": [ "PF01378" ], "CATH": [ "1pgbA00 (3.10.20.10)" ], "MUTATION_PDB": "WT", "T": 21.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 12.02, "ln(ku)_H2O": 12.02, "AUTHOR_KIN": "Mu\u00f1oz V, Thompson PA, Hofrichter J, Eaton WA", "REFERENCE_KIN": "Folding dynamics and mechanism of beta-hairpin formation. Nature. 1997 390(6656):196-9. https://doi.org/10.1038/36626", "YEAR_KIN": 1997.0, "PMID_KIN": "9367160", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "C-terminal \u03b2-hairpin of protein GB", "SOURCE": "Streptococcus sp. group G", "LENGTH": 16.0, "UniProt": "P06654", "PDB_wild": "1pgb", "MUTATED_CHAIN": "A", "PFAM": [ "PF01378" ], "CATH": [ "1pgbA00 (3.10.20.10)" ], "MUTATION_PDB": "WT", "T": 21.0, "pH": 7.0, "BUFFER_NAME": "KPi", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 12.02, "ln(ku)_H2O": 12.02, "AUTHOR_KIN": "Mu\u00f1oz V, Thompson PA, Hofrichter J, Eaton WA", "REFERENCE_KIN": "Folding dynamics and mechanism of beta-hairpin formation. Nature. 1997 390(6656):196-9. https://doi.org/10.1038/36626", "YEAR_KIN": 1997.0, "PMID_KIN": "9367160", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(N239H,Q243W)", "MUTATION_PDB": "WT(N29H,Q33W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.77, "ln(ku)_H2O": 2.13, "dGKIN_H2O": 16.44, "ddGKIN_H2O": -4.06, "mf": -4.18, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(N239H,Q243W)", "MUTATION_PDB": "WT(N29H,Q33W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.77, "ln(ku)_H2O": 2.13, "dGKIN_H2O": 16.44, "ddGKIN_H2O": -4.06, "mf": -4.18, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.08, "dln(kf)_H2O": 0.31, "ln(ku)_H2O": 2.18, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 17.11, "ddGKIN_H2O": -3.39, "mf": -4.52, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.08, "dln(kf)_H2O": 0.31, "ln(ku)_H2O": 2.18, "dln(ku)_H2O": 0.05, "dGKIN_H2O": 17.11, "ddGKIN_H2O": -3.39, "mf": -4.52, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.55, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 3.19, "dln(ku)_H2O": 1.06, "dGKIN_H2O": 13.26, "ddGKIN_H2O": -7.24, "mf": -4.14, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.55, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 3.19, "dln(ku)_H2O": 1.06, "dGKIN_H2O": 13.26, "ddGKIN_H2O": -7.24, "mf": -4.14, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.76, "dln(kf)_H2O": -0.01, "ln(ku)_H2O": 1.95, "dln(ku)_H2O": -0.18, "dGKIN_H2O": 16.86, "ddGKIN_H2O": -3.64, "mf": -3.97, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.76, "dln(kf)_H2O": -0.01, "ln(ku)_H2O": 1.95, "dln(ku)_H2O": -0.18, "dGKIN_H2O": 16.86, "ddGKIN_H2O": -3.64, "mf": -3.97, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.25, "dln(kf)_H2O": -0.52, "ln(ku)_H2O": 3.82, "dln(ku)_H2O": 1.69, "dGKIN_H2O": 11.0, "ddGKIN_H2O": -9.5, "mf": -4.44, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.25, "dln(kf)_H2O": -0.52, "ln(ku)_H2O": 3.82, "dln(ku)_H2O": 1.69, "dGKIN_H2O": 11.0, "ddGKIN_H2O": -9.5, "mf": -4.44, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.55, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 4.41, "dln(ku)_H2O": 2.28, "dGKIN_H2O": 10.25, "ddGKIN_H2O": -10.25, "mf": -4.77, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.55, "dln(kf)_H2O": -0.22, "ln(ku)_H2O": 4.41, "dln(ku)_H2O": 2.28, "dGKIN_H2O": 10.25, "ddGKIN_H2O": -10.25, "mf": -4.77, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.62, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 3.08, "dln(ku)_H2O": 0.95, "dGKIN_H2O": 11.25, "ddGKIN_H2O": -9.25, "mf": -4.73, "mu": 2.01, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.62, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 3.08, "dln(ku)_H2O": 0.95, "dGKIN_H2O": 11.25, "ddGKIN_H2O": -9.25, "mf": -4.73, "mu": 2.01, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L18A", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.88, "dln(kf)_H2O": 0.11, "ln(ku)_H2O": 6.53, "dln(ku)_H2O": 4.4, "dGKIN_H2O": 5.82, "ddGKIN_H2O": -14.68, "mf": -4.77, "mu": 1.97, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L18A", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.88, "dln(kf)_H2O": 0.11, "ln(ku)_H2O": 6.53, "dln(ku)_H2O": 4.4, "dGKIN_H2O": 5.82, "ddGKIN_H2O": -14.68, "mf": -4.77, "mu": 1.97, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.38, "dln(kf)_H2O": -0.39, "ln(ku)_H2O": 3.58, "dln(ku)_H2O": 1.45, "dGKIN_H2O": 11.88, "ddGKIN_H2O": -8.62, "mf": -3.97, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.38, "dln(kf)_H2O": -0.39, "ln(ku)_H2O": 3.58, "dln(ku)_H2O": 1.45, "dGKIN_H2O": 11.88, "ddGKIN_H2O": -8.62, "mf": -3.97, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.14, "dln(kf)_H2O": -1.63, "ln(ku)_H2O": 5.25, "dln(ku)_H2O": 3.12, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -15.81, "mf": -4.64, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 7.14, "dln(kf)_H2O": -1.63, "ln(ku)_H2O": 5.25, "dln(ku)_H2O": 3.12, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -15.81, "mf": -4.64, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.02, "dln(kf)_H2O": -0.75, "ln(ku)_H2O": 3.07, "dln(ku)_H2O": 0.94, "dGKIN_H2O": 12.26, "ddGKIN_H2O": -8.24, "mf": -4.48, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.02, "dln(kf)_H2O": -0.75, "ln(ku)_H2O": 3.07, "dln(ku)_H2O": 0.94, "dGKIN_H2O": 12.26, "ddGKIN_H2O": -8.24, "mf": -4.48, "mu": 1.76, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.79, "dln(kf)_H2O": 1.02, "ln(ku)_H2O": 2.24, "dln(ku)_H2O": 0.11, "dGKIN_H2O": 18.7, "ddGKIN_H2O": -1.8, "mf": -4.9, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.053", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.79, "dln(kf)_H2O": 1.02, "ln(ku)_H2O": 2.24, "dln(ku)_H2O": 0.11, "dGKIN_H2O": 18.7, "ddGKIN_H2O": -1.8, "mf": -4.9, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.053", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.91, "dln(kf)_H2O": 0.14, "ln(ku)_H2O": 2.84, "dln(ku)_H2O": 0.71, "dGKIN_H2O": 15.06, "ddGKIN_H2O": -5.44, "mf": -5.02, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.91, "dln(kf)_H2O": 0.14, "ln(ku)_H2O": 2.84, "dln(ku)_H2O": 0.71, "dGKIN_H2O": 15.06, "ddGKIN_H2O": -5.44, "mf": -5.02, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "9.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.43, "dln(kf)_H2O": -0.34, "ln(ku)_H2O": 4.3, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 10.25, "ddGKIN_H2O": -10.25, "mf": -4.69, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "9.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.43, "dln(kf)_H2O": -0.34, "ln(ku)_H2O": 4.3, "dln(ku)_H2O": 2.17, "dGKIN_H2O": 10.25, "ddGKIN_H2O": -10.25, "mf": -4.69, "mu": 2.13, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Coil", "RSA": "57.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.82, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 2.47, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 15.73, "ddGKIN_H2O": -4.77, "mf": -4.31, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Coil", "RSA": "57.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.82, "dln(kf)_H2O": 0.05, "ln(ku)_H2O": 2.47, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 15.73, "ddGKIN_H2O": -4.77, "mf": -4.31, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.42, "dln(kf)_H2O": 0.65, "ln(ku)_H2O": 1.0, "dln(ku)_H2O": -1.13, "dGKIN_H2O": 20.84, "ddGKIN_H2O": 0.34, "mf": -4.02, "mu": 2.43, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.42, "dln(kf)_H2O": 0.65, "ln(ku)_H2O": 1.0, "dln(ku)_H2O": -1.13, "dGKIN_H2O": 20.84, "ddGKIN_H2O": 0.34, "mf": -4.02, "mu": 2.43, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.06, "dln(kf)_H2O": 0.29, "ln(ku)_H2O": 3.58, "dln(ku)_H2O": 1.45, "dGKIN_H2O": 13.56, "ddGKIN_H2O": -6.94, "mf": -4.52, "mu": 2.01, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.06, "dln(kf)_H2O": 0.29, "ln(ku)_H2O": 3.58, "dln(ku)_H2O": 1.45, "dGKIN_H2O": 13.56, "ddGKIN_H2O": -6.94, "mf": -4.52, "mu": 2.01, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "11.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.33, "dln(kf)_H2O": -0.44, "ln(ku)_H2O": 5.22, "dln(ku)_H2O": 3.09, "dGKIN_H2O": 7.7, "ddGKIN_H2O": -12.8, "mf": -5.44, "mu": 1.63, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "11.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.33, "dln(kf)_H2O": -0.44, "ln(ku)_H2O": 5.22, "dln(ku)_H2O": 3.09, "dGKIN_H2O": 7.7, "ddGKIN_H2O": -12.8, "mf": -5.44, "mu": 1.63, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.41, "dln(kf)_H2O": 0.64, "ln(ku)_H2O": 2.53, "dln(ku)_H2O": 0.4, "dGKIN_H2O": 17.07, "ddGKIN_H2O": -3.43, "mf": -4.85, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.41, "dln(kf)_H2O": 0.64, "ln(ku)_H2O": 2.53, "dln(ku)_H2O": 0.4, "dGKIN_H2O": 17.07, "ddGKIN_H2O": -3.43, "mf": -4.85, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.8, "dln(kf)_H2O": 0.03, "ln(ku)_H2O": 4.05, "dln(ku)_H2O": 1.92, "dGKIN_H2O": 11.8, "ddGKIN_H2O": -8.7, "mf": -4.69, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.061", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.8, "dln(kf)_H2O": 0.03, "ln(ku)_H2O": 4.05, "dln(ku)_H2O": 1.92, "dGKIN_H2O": 11.8, "ddGKIN_H2O": -8.7, "mf": -4.69, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.061", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "31.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.79, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 4.43, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 10.79, "ddGKIN_H2O": -9.71, "mf": -4.56, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (N31H/Q35W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "31.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.79, "dln(kf)_H2O": 0.02, "ln(ku)_H2O": 4.43, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 10.79, "ddGKIN_H2O": -9.71, "mf": -4.56, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "N239H", "Q243W" ], "BACKGROUND_MUT_PDB": [ "N29H", "Q33W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(E258W)", "MUTATION_PDB": "WT(E48W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.29, "ln(ku)_H2O": 3.44, "dGKIN_H2O": 19.46, "ddGKIN_H2O": -1.04, "mf": -4.69, "mu": 1.72, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "WT(E258W)", "MUTATION_PDB": "WT(E48W)", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.29, "ln(ku)_H2O": 3.44, "dGKIN_H2O": 19.46, "ddGKIN_H2O": -1.04, "mf": -4.69, "mu": 1.72, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.96, "dln(kf)_H2O": -0.33, "ln(ku)_H2O": 2.48, "dln(ku)_H2O": -0.96, "dGKIN_H2O": 21.0, "ddGKIN_H2O": 0.5, "mf": -4.18, "mu": 2.18, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222A", "MUTATION_PDB": "N12A", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.96, "dln(kf)_H2O": -0.33, "ln(ku)_H2O": 2.48, "dln(ku)_H2O": -0.96, "dGKIN_H2O": 21.0, "ddGKIN_H2O": 0.5, "mf": -4.18, "mu": 2.18, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.043", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.8, "dln(kf)_H2O": -0.49, "ln(ku)_H2O": 3.98, "dln(ku)_H2O": 0.54, "dGKIN_H2O": 16.9, "ddGKIN_H2O": -3.6, "mf": -4.35, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "N222G", "MUTATION_PDB": "N12G", "SEC_STR": "Helix", "RSA": "47.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.8, "dln(kf)_H2O": -0.49, "ln(ku)_H2O": 3.98, "dln(ku)_H2O": 0.54, "dGKIN_H2O": 16.9, "ddGKIN_H2O": -3.6, "mf": -4.35, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.044", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.41, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 3.62, "dln(ku)_H2O": 0.18, "dGKIN_H2O": 19.33, "ddGKIN_H2O": -1.17, "mf": -4.52, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226A", "MUTATION_PDB": "E16A", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.41, "dln(kf)_H2O": 0.12, "ln(ku)_H2O": 3.62, "dln(ku)_H2O": 0.18, "dGKIN_H2O": 19.33, "ddGKIN_H2O": -1.17, "mf": -4.52, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.045", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.52, "dln(kf)_H2O": -0.77, "ln(ku)_H2O": 4.59, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 14.73, "ddGKIN_H2O": -5.77, "mf": -4.44, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "E226G", "MUTATION_PDB": "E16G", "SEC_STR": "Helix", "RSA": "67.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.52, "dln(kf)_H2O": -0.77, "ln(ku)_H2O": 4.59, "dln(ku)_H2O": 1.15, "dGKIN_H2O": 14.73, "ddGKIN_H2O": -5.77, "mf": -4.44, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.046", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.56, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 4.92, "dln(ku)_H2O": 1.48, "dGKIN_H2O": 13.97, "ddGKIN_H2O": -6.53, "mf": -4.52, "mu": 1.97, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I227V", "MUTATION_PDB": "I17V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.56, "dln(kf)_H2O": -0.73, "ln(ku)_H2O": 4.92, "dln(ku)_H2O": 1.48, "dGKIN_H2O": 13.97, "ddGKIN_H2O": -6.53, "mf": -4.52, "mu": 1.97, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.047", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.97, "dln(kf)_H2O": -1.32, "ln(ku)_H2O": 4.37, "dln(ku)_H2O": 0.93, "dGKIN_H2O": 13.85, "ddGKIN_H2O": -6.65, "mf": -5.19, "mu": 1.8, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L228G", "MUTATION_PDB": "L18G", "SEC_STR": "Coil", "RSA": "26.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.97, "dln(kf)_H2O": -1.32, "ln(ku)_H2O": 4.37, "dln(ku)_H2O": 0.93, "dGKIN_H2O": 13.85, "ddGKIN_H2O": -6.65, "mf": -5.19, "mu": 1.8, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.048", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L20A", "SEC_STR": "Coil", "RSA": "11.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.62, "dln(kf)_H2O": -0.67, "ln(ku)_H2O": 7.29, "dln(ku)_H2O": 3.85, "dGKIN_H2O": 8.24, "ddGKIN_H2O": -12.26, "mf": -4.1, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L230A", "MUTATION_PDB": "L20A", "SEC_STR": "Coil", "RSA": "11.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.62, "dln(kf)_H2O": -0.67, "ln(ku)_H2O": 7.29, "dln(ku)_H2O": 3.85, "dGKIN_H2O": 8.24, "ddGKIN_H2O": -12.26, "mf": -4.1, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.049", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.53, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 5.11, "dln(ku)_H2O": 1.67, "dGKIN_H2O": 13.43, "ddGKIN_H2O": -7.07, "mf": -4.52, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238A", "MUTATION_PDB": "R28A", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.53, "dln(kf)_H2O": -0.76, "ln(ku)_H2O": 5.11, "dln(ku)_H2O": 1.67, "dGKIN_H2O": 13.43, "ddGKIN_H2O": -7.07, "mf": -4.52, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.050", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.91, "dln(kf)_H2O": -2.38, "ln(ku)_H2O": 6.28, "dln(ku)_H2O": 2.84, "dGKIN_H2O": 6.53, "ddGKIN_H2O": -13.97, "mf": -5.65, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "R238G", "MUTATION_PDB": "R28G", "SEC_STR": "Helix", "RSA": "10.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 8.91, "dln(kf)_H2O": -2.38, "ln(ku)_H2O": 6.28, "dln(ku)_H2O": 2.84, "dGKIN_H2O": 6.53, "ddGKIN_H2O": -13.97, "mf": -5.65, "mu": 1.21, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.051", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.44, "dln(kf)_H2O": -0.85, "ln(ku)_H2O": 3.65, "dln(ku)_H2O": 0.21, "dGKIN_H2O": 16.86, "ddGKIN_H2O": -3.64, "mf": -4.31, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "I242V", "MUTATION_PDB": "I32V", "SEC_STR": "Helix", "RSA": "0.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.44, "dln(kf)_H2O": -0.85, "ln(ku)_H2O": 3.65, "dln(ku)_H2O": 0.21, "dGKIN_H2O": 16.86, "ddGKIN_H2O": -3.64, "mf": -4.31, "mu": 2.22, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.052", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.48, "dln(kf)_H2O": 0.19, "ln(ku)_H2O": 2.4, "dln(ku)_H2O": -1.04, "dGKIN_H2O": 22.51, "ddGKIN_H2O": 2.01, "mf": -4.18, "mu": 1.92, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.053", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244A", "MUTATION_PDB": "S34A", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.48, "dln(kf)_H2O": 0.19, "ln(ku)_H2O": 2.4, "dln(ku)_H2O": -1.04, "dGKIN_H2O": 22.51, "ddGKIN_H2O": 2.01, "mf": -4.18, "mu": 1.92, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.053", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -0.88, "ln(ku)_H2O": 3.52, "dln(ku)_H2O": 0.08, "dGKIN_H2O": 17.07, "ddGKIN_H2O": -3.43, "mf": -4.44, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S244G", "MUTATION_PDB": "S34G", "SEC_STR": "Helix", "RSA": "30.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.41, "dln(kf)_H2O": -0.88, "ln(ku)_H2O": 3.52, "dln(ku)_H2O": 0.08, "dGKIN_H2O": 17.07, "ddGKIN_H2O": -3.43, "mf": -4.44, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.054", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "9.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.95, "dln(kf)_H2O": -1.34, "ln(ku)_H2O": 5.64, "dln(ku)_H2O": 2.2, "dGKIN_H2O": 10.71, "ddGKIN_H2O": -9.79, "mf": -5.31, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L245A", "MUTATION_PDB": "L35A", "SEC_STR": "Helix", "RSA": "9.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 9.95, "dln(kf)_H2O": -1.34, "ln(ku)_H2O": 5.64, "dln(ku)_H2O": 2.2, "dGKIN_H2O": 10.71, "ddGKIN_H2O": -9.79, "mf": -5.31, "mu": 1.84, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.055", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Coil", "RSA": "57.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.12, "dln(kf)_H2O": -0.17, "ln(ku)_H2O": 4.49, "dln(ku)_H2O": 1.05, "dGKIN_H2O": 16.4, "ddGKIN_H2O": -4.1, "mf": -4.77, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S250G", "MUTATION_PDB": "S40G", "SEC_STR": "Coil", "RSA": "57.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.12, "dln(kf)_H2O": -0.17, "ln(ku)_H2O": 4.49, "dln(ku)_H2O": 1.05, "dGKIN_H2O": 16.4, "ddGKIN_H2O": -4.1, "mf": -4.77, "mu": 1.38, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.056", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.1, "dln(kf)_H2O": 0.81, "ln(ku)_H2O": 3.32, "dln(ku)_H2O": -0.12, "dGKIN_H2O": 21.76, "ddGKIN_H2O": 1.26, "mf": -4.73, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252A", "MUTATION_PDB": "S42A", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 12.1, "dln(kf)_H2O": 0.81, "ln(ku)_H2O": 3.32, "dln(ku)_H2O": -0.12, "dGKIN_H2O": 21.76, "ddGKIN_H2O": 1.26, "mf": -4.73, "mu": 1.55, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.057", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.06, "dln(kf)_H2O": -0.23, "ln(ku)_H2O": 4.66, "dln(ku)_H2O": 1.22, "dGKIN_H2O": 15.86, "ddGKIN_H2O": -4.64, "mf": -4.56, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "S252G", "MUTATION_PDB": "S42G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.06, "dln(kf)_H2O": -0.23, "ln(ku)_H2O": 4.66, "dln(ku)_H2O": 1.22, "dGKIN_H2O": 15.86, "ddGKIN_H2O": -4.64, "mf": -4.56, "mu": 1.88, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.058", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "11.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.42, "dln(kf)_H2O": -0.87, "ln(ku)_H2O": 5.73, "dln(ku)_H2O": 2.29, "dGKIN_H2O": 11.63, "ddGKIN_H2O": -8.87, "mf": -5.19, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L255A", "MUTATION_PDB": "L45A", "SEC_STR": "Helix", "RSA": "11.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.42, "dln(kf)_H2O": -0.87, "ln(ku)_H2O": 5.73, "dln(ku)_H2O": 2.29, "dGKIN_H2O": 11.63, "ddGKIN_H2O": -8.87, "mf": -5.19, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.059", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.64, "dln(kf)_H2O": 0.35, "ln(ku)_H2O": 3.16, "dln(ku)_H2O": -0.28, "dGKIN_H2O": 21.0, "ddGKIN_H2O": 0.5, "mf": -4.69, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261A", "MUTATION_PDB": "K51A", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 11.64, "dln(kf)_H2O": 0.35, "ln(ku)_H2O": 3.16, "dln(ku)_H2O": -0.28, "dGKIN_H2O": 21.0, "ddGKIN_H2O": 0.5, "mf": -4.69, "mu": 1.67, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.060", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.91, "dln(kf)_H2O": -0.38, "ln(ku)_H2O": 5.14, "dln(ku)_H2O": 1.7, "dGKIN_H2O": 14.31, "ddGKIN_H2O": -6.19, "mf": -4.35, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.061", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "K261G", "MUTATION_PDB": "K51G", "SEC_STR": "Helix", "RSA": "80.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.91, "dln(kf)_H2O": -0.38, "ln(ku)_H2O": 5.14, "dln(ku)_H2O": 1.7, "dGKIN_H2O": 14.31, "ddGKIN_H2O": -6.19, "mf": -4.35, "mu": 1.59, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.061", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "31.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.83, "dln(kf)_H2O": -0.46, "ln(ku)_H2O": 5.73, "dln(ku)_H2O": 2.29, "dGKIN_H2O": 12.64, "ddGKIN_H2O": -7.86, "mf": -4.85, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "BdpA (E50W)", "SOURCE": "Staphylococcus aureus", "LENGTH": 60.0, "UniProt": "P38507", "PDB_wild": "1bdc", "MUTATED_CHAIN": "A", "PFAM": [ "PF02216" ], "CATH": [ "1bdcA00 (1.20.5.420)" ], "MUTATION_UNIPROT": "L262A", "MUTATION_PDB": "L52A", "SEC_STR": "Helix", "RSA": "31.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal", "ln(kf)_H2O": 10.83, "dln(kf)_H2O": -0.46, "ln(ku)_H2O": 5.73, "dln(ku)_H2O": 2.29, "dGKIN_H2O": 12.64, "ddGKIN_H2O": -7.86, "mf": -4.85, "mu": 1.51, "BACKGROUND_MUT_UNIPROT": [ "E258W" ], "BACKGROUND_MUT_PDB": [ "E48W" ], "AUTHOR_KIN": "Sato, S., Fersht, A.R.", "REFERENCE_KIN": "Searching for multiple folding pathways of a nearly symmetrical protein: temperature dependent phi-value analysis of the B domain of protein A. J. Mol. Biol. 372, 254\u2013267. https://doi.org/10.1016/j.jmb.2007.06.062", "YEAR_KIN": 2007.0, "PMID_KIN": "17628591", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Trp-cage protein", "LENGTH": 20.0, "PDB_wild": "1l2y", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT", "T": 23.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.1", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 12.39, "ln(ku)_H2O": 11.32, "dGKIN_H2O": 2.62, "AUTHOR_KIN": "Qiu L, Pabit SA, Roitberg AE, Hagen SJ", "REFERENCE_KIN": "Smaller and faster: the 20-residue Trp-cage protein folds in 4 micros. J Am Chem Soc. 124(44):12952-3. https://doi.org/10.1021/ja0279141", "YEAR_KIN": 2002.0, "PMID_KIN": "12405814", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "Trp-cage protein", "LENGTH": 20.0, "PDB_wild": "1l2y", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT", "T": 23.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.1", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "ln(kf)_H2O": 12.39, "ln(ku)_H2O": 11.32, "dGKIN_H2O": 2.62, "AUTHOR_KIN": "Qiu L, Pabit SA, Roitberg AE, Hagen SJ", "REFERENCE_KIN": "Smaller and faster: the 20-residue Trp-cage protein folds in 4 micros. J Am Chem Soc. 124(44):12952-3. https://doi.org/10.1021/ja0279141", "YEAR_KIN": 2002.0, "PMID_KIN": "12405814", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "\u03b13D", "LENGTH": 73.0, "PDB_wild": "2a3d", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT", "T": 25.0, "pH": 2.2, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation or urea", "ln(kf)_H2O": 12.24, "ln(ku)_H2O": 7.83, "dGKIN_H2O": 10.93, "AUTHOR_KIN": "Zhu Y, Alonso DO, Maki K, Huang CY, Lahr SJ, Daggett V, Roder H, DeGrado WF, Gai F", "REFERENCE_KIN": "Ultrafast folding of alpha3D: a de novo designed three-helix bundle protein. Proc Natl Acad Sci U S A. 100(26):15486-91. https://doi.org/10.1073/pnas.2136623100", "YEAR_KIN": 2003.0, "PMID_KIN": "14671331", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "\u03b13D", "LENGTH": 73.0, "PDB_wild": "2a3d", "MUTATED_CHAIN": "A", "MUTATION_PDB": "WT", "T": 25.0, "pH": 2.2, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation or urea", "ln(kf)_H2O": 12.24, "ln(ku)_H2O": 7.83, "dGKIN_H2O": 10.93, "AUTHOR_KIN": "Zhu Y, Alonso DO, Maki K, Huang CY, Lahr SJ, Daggett V, Roder H, DeGrado WF, Gai F", "REFERENCE_KIN": "Ultrafast folding of alpha3D: a de novo designed three-helix bundle protein. Proc Natl Acad Sci U S A. 100(26):15486-91. https://doi.org/10.1073/pnas.2136623100", "YEAR_KIN": 2003.0, "PMID_KIN": "14671331", "STATE": "Yes", "REVIEW_DATE": "2023-06-09T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "K130G", "MUTATION_PDB": "K164G", "SEC_STR": "Helix", "RSA": "61.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": 7.75, "dln(ku)_H2O": 1.4, "dGKIN_H2O": 7.29, "ddGKIN_H2O": -7.35, "mf": -3.05, "mu": 0.63, "PhiF_H2O": 0.57, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.111", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "K130G", "MUTATION_PDB": "K164G", "SEC_STR": "Helix", "RSA": "61.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": 7.75, "dln(ku)_H2O": 1.4, "dGKIN_H2O": 7.29, "ddGKIN_H2O": -7.35, "mf": -3.05, "mu": 0.63, "PhiF_H2O": 0.57, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.111", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "R131G", "MUTATION_PDB": "R165G", "SEC_STR": "Helix", "RSA": "56.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": 7.26, "dln(ku)_H2O": 0.91, "dGKIN_H2O": 8.51, "ddGKIN_H2O": -6.13, "mf": -2.72, "mu": 0.71, "PhiF_H2O": 0.76, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.113", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "R131G", "MUTATION_PDB": "R165G", "SEC_STR": "Helix", "RSA": "56.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.69, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": 7.26, "dln(ku)_H2O": 0.91, "dGKIN_H2O": 8.51, "ddGKIN_H2O": -6.13, "mf": -2.72, "mu": 0.71, "PhiF_H2O": 0.76, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.113", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A133G", "MUTATION_PDB": "A167G", "SEC_STR": "Helix", "RSA": "19.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.03, "dln(kf)_H2O": -1.22, "ln(ku)_H2O": 7.54, "dln(ku)_H2O": 1.19, "dGKIN_H2O": 8.66, "ddGKIN_H2O": -5.98, "mf": -2.64, "mu": 0.71, "PhiF_H2O": 0.64, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.114", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A133G", "MUTATION_PDB": "A167G", "SEC_STR": "Helix", "RSA": "19.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.03, "dln(kf)_H2O": -1.22, "ln(ku)_H2O": 7.54, "dln(ku)_H2O": 1.19, "dGKIN_H2O": 8.66, "ddGKIN_H2O": -5.98, "mf": -2.64, "mu": 0.71, "PhiF_H2O": 0.64, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.114", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A97G", "MUTATION_PDB": "A131G", "SEC_STR": "Coil", "RSA": "24.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.98, "dln(kf)_H2O": -0.27, "ln(ku)_H2O": 8.19, "dln(ku)_H2O": 1.84, "dGKIN_H2O": 9.4, "ddGKIN_H2O": -5.24, "mf": -2.64, "mu": 0.63, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.082", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A97G", "MUTATION_PDB": "A131G", "SEC_STR": "Coil", "RSA": "24.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.98, "dln(kf)_H2O": -0.27, "ln(ku)_H2O": 8.19, "dln(ku)_H2O": 1.84, "dGKIN_H2O": 9.4, "ddGKIN_H2O": -5.24, "mf": -2.64, "mu": 0.63, "PhiF_H2O": 0.2, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.082", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "M98A", "MUTATION_PDB": "M132A", "SEC_STR": "Coil", "RSA": "28.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.78, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 7.38, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 10.9, "ddGKIN_H2O": -3.74, "mf": -2.72, "mu": 0.67, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.083", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "M98A", "MUTATION_PDB": "M132A", "SEC_STR": "Coil", "RSA": "28.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.78, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 7.38, "dln(ku)_H2O": 1.03, "dGKIN_H2O": 10.9, "ddGKIN_H2O": -3.74, "mf": -2.72, "mu": 0.67, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.083", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A100G", "MUTATION_PDB": "A134G", "SEC_STR": "Helix", "RSA": "44.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.85, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 7.18, "dln(ku)_H2O": 0.83, "dGKIN_H2O": 11.57, "ddGKIN_H2O": -3.07, "mf": -2.85, "mu": 0.67, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.084", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A100G", "MUTATION_PDB": "A134G", "SEC_STR": "Helix", "RSA": "44.3", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.85, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 7.18, "dln(ku)_H2O": 0.83, "dGKIN_H2O": 11.57, "ddGKIN_H2O": -3.07, "mf": -2.85, "mu": 0.67, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.084", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A101G", "MUTATION_PDB": "A135G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.61, "dln(kf)_H2O": -0.64, "ln(ku)_H2O": 9.21, "dln(ku)_H2O": 2.86, "dGKIN_H2O": 5.94, "ddGKIN_H2O": -8.7, "mf": -2.55, "mu": 0.59, "PhiF_H2O": 0.18, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.085", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A101G", "MUTATION_PDB": "A135G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.61, "dln(kf)_H2O": -0.64, "ln(ku)_H2O": 9.21, "dln(ku)_H2O": 2.86, "dGKIN_H2O": 5.94, "ddGKIN_H2O": -8.7, "mf": -2.55, "mu": 0.59, "PhiF_H2O": 0.18, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.085", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L104A", "MUTATION_PDB": "L138A", "SEC_STR": "Helix", "RSA": "17.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.29, "dln(kf)_H2O": -0.96, "ln(ku)_H2O": 8.85, "dln(ku)_H2O": 2.5, "dGKIN_H2O": 6.04, "ddGKIN_H2O": -8.6, "mf": -2.97, "mu": 0.33, "PhiF_H2O": 0.33, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.086", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L104A", "MUTATION_PDB": "L138A", "SEC_STR": "Helix", "RSA": "17.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.29, "dln(kf)_H2O": -0.96, "ln(ku)_H2O": 8.85, "dln(ku)_H2O": 2.5, "dGKIN_H2O": 6.04, "ddGKIN_H2O": -8.6, "mf": -2.97, "mu": 0.33, "PhiF_H2O": 0.33, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.086", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A105G", "MUTATION_PDB": "A139G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.07, "dln(kf)_H2O": -1.18, "ln(ku)_H2O": 9.1, "dln(ku)_H2O": 2.75, "dGKIN_H2O": 4.86, "ddGKIN_H2O": -9.78, "mf": -2.55, "mu": 0.59, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.087", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A105G", "MUTATION_PDB": "A139G", "SEC_STR": "Helix", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.07, "dln(kf)_H2O": -1.18, "ln(ku)_H2O": 9.1, "dln(ku)_H2O": 2.75, "dGKIN_H2O": 4.86, "ddGKIN_H2O": -9.78, "mf": -2.55, "mu": 0.59, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.087", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L108A", "MUTATION_PDB": "L142A", "SEC_STR": "Helix", "RSA": "58.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.1, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 9.31, "dln(ku)_H2O": 2.96, "dGKIN_H2O": 4.44, "ddGKIN_H2O": -10.2, "mf": -2.93, "mu": 0.59, "PhiF_H2O": 0.28, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.088", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "L108A", "MUTATION_PDB": "L142A", "SEC_STR": "Helix", "RSA": "58.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.1, "dln(kf)_H2O": -1.15, "ln(ku)_H2O": 9.31, "dln(ku)_H2O": 2.96, "dGKIN_H2O": 4.44, "ddGKIN_H2O": -10.2, "mf": -2.93, "mu": 0.59, "PhiF_H2O": 0.28, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.088", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I110A", "MUTATION_PDB": "I144A", "SEC_STR": "Coil", "RSA": "11.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.08, "dln(kf)_H2O": -1.17, "ln(ku)_H2O": 8.96, "dln(ku)_H2O": 2.61, "dGKIN_H2O": 5.25, "ddGKIN_H2O": -9.39, "mf": -2.38, "mu": 0.59, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.089", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I110A", "MUTATION_PDB": "I144A", "SEC_STR": "Coil", "RSA": "11.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.08, "dln(kf)_H2O": -1.17, "ln(ku)_H2O": 8.96, "dln(ku)_H2O": 2.61, "dGKIN_H2O": 5.25, "ddGKIN_H2O": -9.39, "mf": -2.38, "mu": 0.59, "PhiF_H2O": 0.31, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.089", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A112G", "MUTATION_PDB": "A146G", "SEC_STR": "Coil", "RSA": "8.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.47, "dln(kf)_H2O": -0.78, "ln(ku)_H2O": 8.72, "dln(ku)_H2O": 2.37, "dGKIN_H2O": 6.83, "ddGKIN_H2O": -7.81, "mf": -2.8, "mu": 0.46, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.091", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "A112G", "MUTATION_PDB": "A146G", "SEC_STR": "Coil", "RSA": "8.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.47, "dln(kf)_H2O": -0.78, "ln(ku)_H2O": 8.72, "dln(ku)_H2O": 2.37, "dGKIN_H2O": 6.83, "ddGKIN_H2O": -7.81, "mf": -2.8, "mu": 0.46, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.091", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V115A", "MUTATION_PDB": "V149A", "SEC_STR": "Coil", "RSA": "2.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.29, "dln(kf)_H2O": -0.96, "ln(ku)_H2O": 8.1, "dln(ku)_H2O": 1.75, "dGKIN_H2O": 7.9, "ddGKIN_H2O": -6.74, "mf": -3.18, "mu": 0.59, "PhiF_H2O": 0.38, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.094", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V115A", "MUTATION_PDB": "V149A", "SEC_STR": "Coil", "RSA": "2.8", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.29, "dln(kf)_H2O": -0.96, "ln(ku)_H2O": 8.1, "dln(ku)_H2O": 1.75, "dGKIN_H2O": 7.9, "ddGKIN_H2O": -6.74, "mf": -3.18, "mu": 0.59, "PhiF_H2O": 0.38, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.094", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "T118S", "MUTATION_PDB": "T152S", "SEC_STR": "Coil", "RSA": "73.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.78, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 7.39, "dln(ku)_H2O": 1.04, "dGKIN_H2O": 10.86, "ddGKIN_H2O": -3.78, "mf": -2.89, "mu": 0.59, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.096", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "T118S", "MUTATION_PDB": "T152S", "SEC_STR": "Coil", "RSA": "73.9", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.78, "dln(kf)_H2O": -0.47, "ln(ku)_H2O": 7.39, "dln(ku)_H2O": 1.04, "dGKIN_H2O": 10.86, "ddGKIN_H2O": -3.78, "mf": -2.89, "mu": 0.59, "PhiF_H2O": 0.4, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.096", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "P120A", "MUTATION_PDB": "P154A", "SEC_STR": "Coil", "RSA": "90.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.16, "dln(kf)_H2O": -1.09, "ln(ku)_H2O": 7.01, "dln(ku)_H2O": 0.66, "dGKIN_H2O": 10.27, "ddGKIN_H2O": -4.37, "mf": -2.76, "mu": 0.63, "PhiF_H2O": 0.8, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.097", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "P120A", "MUTATION_PDB": "P154A", "SEC_STR": "Coil", "RSA": "90.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.16, "dln(kf)_H2O": -1.09, "ln(ku)_H2O": 7.01, "dln(ku)_H2O": 0.66, "dGKIN_H2O": 10.27, "ddGKIN_H2O": -4.37, "mf": -2.76, "mu": 0.63, "PhiF_H2O": 0.8, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.097", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "P120G", "MUTATION_PDB": "P154G", "SEC_STR": "Coil", "RSA": "90.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.55, "dln(kf)_H2O": -1.7, "ln(ku)_H2O": 7.86, "dln(ku)_H2O": 1.51, "dGKIN_H2O": 6.65, "ddGKIN_H2O": -7.99, "mf": -2.93, "mu": 0.75, "PhiF_H2O": 0.58, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.098", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "P120G", "MUTATION_PDB": "P154G", "SEC_STR": "Coil", "RSA": "90.4", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.55, "dln(kf)_H2O": -1.7, "ln(ku)_H2O": 7.86, "dln(ku)_H2O": 1.51, "dGKIN_H2O": 6.65, "ddGKIN_H2O": -7.99, "mf": -2.93, "mu": 0.75, "PhiF_H2O": 0.58, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.098", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V123A", "MUTATION_PDB": "V157A", "SEC_STR": "Beta", "RSA": "15.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.85, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 10.18, "ddGKIN_H2O": -4.46, "mf": -2.76, "mu": 0.54, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.099", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V123A", "MUTATION_PDB": "V157A", "SEC_STR": "Beta", "RSA": "15.5", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.85, "dln(kf)_H2O": -0.4, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 10.18, "ddGKIN_H2O": -4.46, "mf": -2.76, "mu": 0.54, "PhiF_H2O": 0.3, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.099", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I124A", "MUTATION_PDB": "I158A", "SEC_STR": "Coil", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.82, "dln(kf)_H2O": -1.43, "ln(ku)_H2O": 8.07, "dln(ku)_H2O": 1.72, "dGKIN_H2O": 6.81, "ddGKIN_H2O": -7.83, "mf": -2.93, "mu": 0.59, "PhiF_H2O": 0.45, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.100", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "I124A", "MUTATION_PDB": "I158A", "SEC_STR": "Coil", "RSA": "0.0", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.82, "dln(kf)_H2O": -1.43, "ln(ku)_H2O": 8.07, "dln(ku)_H2O": 1.72, "dGKIN_H2O": 6.81, "ddGKIN_H2O": -7.83, "mf": -2.93, "mu": 0.59, "PhiF_H2O": 0.45, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.100", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "T125A", "MUTATION_PDB": "T159A", "SEC_STR": "Coil", "RSA": "21.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.93, "dln(kf)_H2O": -1.32, "ln(ku)_H2O": 7.94, "dln(ku)_H2O": 1.59, "dGKIN_H2O": 7.42, "ddGKIN_H2O": -7.22, "mf": -3.05, "mu": 0.71, "PhiF_H2O": 0.48, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.102", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "T125A", "MUTATION_PDB": "T159A", "SEC_STR": "Coil", "RSA": "21.1", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 10.93, "dln(kf)_H2O": -1.32, "ln(ku)_H2O": 7.94, "dln(ku)_H2O": 1.59, "dGKIN_H2O": 7.42, "ddGKIN_H2O": -7.22, "mf": -3.05, "mu": 0.71, "PhiF_H2O": 0.48, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.102", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V126A", "MUTATION_PDB": "V160A", "SEC_STR": "Helix", "RSA": "31.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.92, "dln(kf)_H2O": -0.33, "ln(ku)_H2O": 8.39, "dln(ku)_H2O": 2.04, "dGKIN_H2O": 8.74, "ddGKIN_H2O": -5.9, "mf": -2.72, "mu": 0.42, "PhiF_H2O": 0.21, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.104", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "V126A", "MUTATION_PDB": "V160A", "SEC_STR": "Helix", "RSA": "31.7", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.92, "dln(kf)_H2O": -0.33, "ln(ku)_H2O": 8.39, "dln(ku)_H2O": 2.04, "dGKIN_H2O": 8.74, "ddGKIN_H2O": -5.9, "mf": -2.72, "mu": 0.42, "PhiF_H2O": 0.21, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.104", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E127G", "MUTATION_PDB": "E161G", "SEC_STR": "Helix", "RSA": "58.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.14, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 0.51, "dGKIN_H2O": 10.62, "ddGKIN_H2O": -4.02, "mf": -2.89, "mu": 0.5, "PhiF_H2O": 0.9, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.106", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E127G", "MUTATION_PDB": "E161G", "SEC_STR": "Helix", "RSA": "58.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.14, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 0.51, "dGKIN_H2O": 10.62, "ddGKIN_H2O": -4.02, "mf": -2.89, "mu": 0.5, "PhiF_H2O": 0.9, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.106", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D128N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "16.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -2.61, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -9.95, "mf": -2.64, "mu": 0.67, "PhiF_H2O": 0.65, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.108", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D128N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "16.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -2.61, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -9.95, "mf": -2.64, "mu": 0.67, "PhiF_H2O": 0.65, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.108", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E436A", "MUTATION_PDB": "E7A", "SEC_STR": "Coil", "RSA": "48.5", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.43, "dln(kf)_H2O": -0.62, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 7.74, "ddGKIN_H2O": -2.18, "mf": -2.34, "mu": 1.64, "PhiF_H2O": 0.67, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.056", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "E436A", "MUTATION_PDB": "E7A", "SEC_STR": "Coil", "RSA": "48.5", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.43, "dln(kf)_H2O": -0.62, "ln(ku)_H2O": 6.13, "dln(ku)_H2O": 0.3, "dGKIN_H2O": 7.74, "ddGKIN_H2O": -2.18, "mf": -2.34, "mu": 1.64, "PhiF_H2O": 0.67, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.056", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "W437F", "MUTATION_PDB": "W8F", "SEC_STR": "Coil", "RSA": "15.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.35, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 8.08, "dln(ku)_H2O": 2.25, "dGKIN_H2O": 2.97, "ddGKIN_H2O": -6.9, "mf": -3.05, "mu": 3.19, "PhiF_H2O": 0.24, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.057", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "W437F", "MUTATION_PDB": "W8F", "SEC_STR": "Coil", "RSA": "15.9", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.35, "dln(kf)_H2O": -0.7, "ln(ku)_H2O": 8.08, "dln(ku)_H2O": 2.25, "dGKIN_H2O": 2.97, "ddGKIN_H2O": -6.9, "mf": -3.05, "mu": 3.19, "PhiF_H2O": 0.24, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.057", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438A", "MUTATION_PDB": "T9A", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.2, "dln(kf)_H2O": 0.15, "ln(ku)_H2O": 7.65, "dln(ku)_H2O": 1.82, "dGKIN_H2O": 5.98, "ddGKIN_H2O": -3.89, "mf": -3.18, "mu": 1.23, "PhiF_H2O": -0.09, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.058", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438A", "MUTATION_PDB": "T9A", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 10.2, "dln(kf)_H2O": 0.15, "ln(ku)_H2O": 7.65, "dln(ku)_H2O": 1.82, "dGKIN_H2O": 5.98, "ddGKIN_H2O": -3.89, "mf": -3.18, "mu": 1.23, "PhiF_H2O": -0.09, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.058", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438G", "MUTATION_PDB": "T9G", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.22, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 5.89, "dln(ku)_H2O": 0.06, "dGKIN_H2O": 7.82, "ddGKIN_H2O": -2.09, "mf": -3.35, "mu": 1.88, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.059", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "FBP28", "SOURCE": "Mus musculus", "LENGTH": 37.0, "UniProt": "Q8CGF7", "PDB_wild": "1e0l", "MUTATED_CHAIN": "A", "PFAM": [ "PF00397" ], "MUTATION_UNIPROT": "T438G", "MUTATION_PDB": "T9G", "SEC_STR": "Beta", "RSA": "33.1", "T": 10.0, "pH": 6.5, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.02", "MEASURE_KIN": "T-jump", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 9.22, "dln(kf)_H2O": -0.83, "ln(ku)_H2O": 5.89, "dln(ku)_H2O": 0.06, "dGKIN_H2O": 7.82, "ddGKIN_H2O": -2.09, "mf": -3.35, "mu": 1.88, "AUTHOR_KIN": "Ferguson, N., Johnson, C.M., Macias, M., Oschkinat, H., Fersht, A.", "REFERENCE_KIN": "Petrovich, M., Jonsson, A.L., Ferguson, N., Daggett, V., Fersht, A.R., 2006. Phi-analysis at the experimental limits: mechanism of beta-hairpin formation. J. Mol. Biol. 360, 865\u2013881. https://doi.org/10.1016/j.jmb.2006.05.059", "YEAR_KIN": 2006.0, "PMID_KIN": "16784750", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" } ]