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ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "CBP, KIX domain", "SOURCE": "Mus musculus", "LENGTH": 86.0, "UniProt": "P45481", "PDB_wild": "1sb0", "MUTATED_CHAIN": "A", "PFAM": [ "PF02172" ], "CATH": [ "1sb0A00 (1.10.246.20)" ], "EC_NUMBER": "2.3.1.48", "MUTATION_UNIPROT": "L628,L652A", "MUTATION_PDB": "L43A,L67A", "SEC_STR": "Helix,Helix", "RSA": "17.7,23.2", "T": 20.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 6.49, "dln(kf)_H2O": -1.29, "ln(ku)_H2O": 0.34, "dln(ku)_H2O": -2.31, "dGKIN_H2O": 14.98, "ddGKIN_H2O": 2.48, "AUTHOR_KIN": "Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S", "REFERENCE_KIN": "The Folding Pathway of the KIX Domain. 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ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "CBP, KIX domain", "SOURCE": "Mus musculus", "LENGTH": 86.0, "UniProt": "P45481", "PDB_wild": "1sb0", "MUTATED_CHAIN": "A", "PFAM": [ "PF02172" ], "CATH": [ "1sb0A00 (1.10.246.20)" ], "EC_NUMBER": "2.3.1.48", "MUTATION_UNIPROT": "Y650,Y658A", "MUTATION_PDB": "Y65A,Y73A", "SEC_STR": "Helix,Helix", "RSA": "41.0,57.7", "T": 20.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.55, "dln(kf)_H2O": -0.23, "ln(ku)_H2O": 3.43, "dln(ku)_H2O": 0.78, "dGKIN_H2O": 10.04, "ddGKIN_H2O": -2.46, "AUTHOR_KIN": "Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S", "REFERENCE_KIN": "The Folding Pathway of the KIX Domain. ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "CBP, KIX domain", "SOURCE": "Mus musculus", "LENGTH": 86.0, "UniProt": "P45481", "PDB_wild": "1sb0", "MUTATED_CHAIN": "A", "PFAM": [ "PF02172" ], "CATH": [ "1sb0A00 (1.10.246.20)" ], "EC_NUMBER": "2.3.1.48", "MUTATION_UNIPROT": "Y650,Y658A", "MUTATION_PDB": "Y65A,Y73A", "SEC_STR": "Helix,Helix", "RSA": "41.0,57.7", "T": 20.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.55, "dln(kf)_H2O": -0.23, "ln(ku)_H2O": 3.43, "dln(ku)_H2O": 0.78, "dGKIN_H2O": 10.04, "ddGKIN_H2O": -2.46, "AUTHOR_KIN": "Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S", "REFERENCE_KIN": "The Folding Pathway of the KIX Domain. ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "CBP, KIX domain", "SOURCE": "Mus musculus", "LENGTH": 86.0, "UniProt": "P45481", "PDB_wild": "1sb0", "MUTATED_CHAIN": "A", "PFAM": [ "PF02172" ], "CATH": [ "1sb0A00 (1.10.246.20)" ], "EC_NUMBER": "2.3.1.48", "MUTATION_UNIPROT": "L652,Y658A", "MUTATION_PDB": "L67A,Y73A", "SEC_STR": "Helix,Helix", "RSA": "23.2,57.7", "T": 20.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.44, "dln(kf)_H2O": -0.34, "ln(ku)_H2O": 3.26, "dln(ku)_H2O": 0.61, "dGKIN_H2O": 10.18, "ddGKIN_H2O": -2.32, "AUTHOR_KIN": "Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S", "REFERENCE_KIN": "The Folding Pathway of the KIX Domain. ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "CBP, KIX domain", "SOURCE": "Mus musculus", "LENGTH": 86.0, "UniProt": "P45481", "PDB_wild": "1sb0", "MUTATED_CHAIN": "A", "PFAM": [ "PF02172" ], "CATH": [ "1sb0A00 (1.10.246.20)" ], "EC_NUMBER": "2.3.1.48", "MUTATION_UNIPROT": "L652,Y658A", "MUTATION_PDB": "L67A,Y73A", "SEC_STR": "Helix,Helix", "RSA": "23.2,57.7", "T": 20.0, "pH": 7.2, "BUFFER_NAME": "Sodium Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 7.44, "dln(kf)_H2O": -0.34, "ln(ku)_H2O": 3.26, "dln(ku)_H2O": 0.61, "dGKIN_H2O": 10.18, "ddGKIN_H2O": -2.32, "AUTHOR_KIN": "Troilo F, Bonetti D, Toto A, Visconti L, Brunori M, Longhi S, Gianni S", "REFERENCE_KIN": "The Folding Pathway of the KIX Domain. ACS Chem Biol. 12(6):1683-1690. https://doi.org/10.1021/acschembio.7b00289", "YEAR_KIN": 2017.0, "PMID_KIN": "28459531", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Tenascin, TNfn3 domain", "SOURCE": "Homo sapiens", "LENGTH": 92.0, "UniProt": "P24821", "PDB_wild": "1ten", "MUTATED_CHAIN": "A", "PFAM": [ "PF00041" ], "CATH": [ "1tenA00 (2.60.40.10)" ], "MUTATION_UNIPROT": "I860A,V871A", "MUTATION_PDB": "I860A,V871A", "SEC_STR": "Beta,Beta", "RSA": "1.8,0.0", "T": 25.0, "pH": 5.0, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "alkaline unfolding (pH 12.4)", "ln(kf)_H2O": -2.3, "dln(kf)_H2O": -3.99, "ln(ku)_H2O": -5.74, "dln(ku)_H2O": 3.88, "PhiF_H2O": 0.51, "AUTHOR_KIN": "Hamill SJ, Steward A, Clarke J", "REFERENCE_KIN": "The folding of an immunoglobulin-like Greek key protein is defined by a common-core nucleus and regions constrained by topology. J Mol Biol. 297(1):165-78. https://doi.org/10.1006/jmbi.2000.3538", "YEAR_KIN": 2000.0, "PMID_KIN": "10704314", "STATE": "Yes", "REVERSIBILITY": "Yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Tenascin, TNfn3 domain", "SOURCE": "Homo sapiens", "LENGTH": 92.0, "UniProt": "P24821", "PDB_wild": "1ten", "MUTATED_CHAIN": "A", "PFAM": [ "PF00041" ], "CATH": [ "1tenA00 (2.60.40.10)" ], "MUTATION_UNIPROT": "I860A,V871A", "MUTATION_PDB": "I860A,V871A", "SEC_STR": "Beta,Beta", "RSA": "1.8,0.0", "T": 25.0, "pH": 5.0, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "alkaline unfolding (pH 12.4)", "ln(kf)_H2O": -2.3, "dln(kf)_H2O": -3.99, "ln(ku)_H2O": -5.74, "dln(ku)_H2O": 3.88, "PhiF_H2O": 0.51, "AUTHOR_KIN": "Hamill SJ, Steward A, Clarke J", "REFERENCE_KIN": "The folding of an immunoglobulin-like Greek key protein is defined by a common-core nucleus and regions constrained by topology. J Mol Biol. 297(1):165-78. https://doi.org/10.1006/jmbi.2000.3538", "YEAR_KIN": 2000.0, "PMID_KIN": "10704314", "STATE": "Yes", "REVERSIBILITY": "Yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A,V11A", "SEC_STR": "Helix,Helix", "RSA": "45.8,61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "SAP (L31W) domain of THO1", "SOURCE": "Saccharomyces cerevisiae", "LENGTH": 49.0, "UniProt": "P40040", "PDB_wild": "2wqg", "MUTATED_CHAIN": "A", "PFAM": [ "PF02037" ], "CATH": [ "2wqgA00 (1.10.720.30)" ], "MUTATION_UNIPROT": "V9A", "MUTATION_PDB": "V10A,V11A", "SEC_STR": "Helix,Helix", "RSA": "45.8,61.3", "T": 10.0, "pH": 6.0, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump", "METHOD_KIN": "thermal denaturation", "BACKGROUND_MUT_UNIPROT": [ "L30W" ], "BACKGROUND_MUT_PDB": [ "L31W" ], "AUTHOR_KIN": "Dodson CA, Arbely E", "REFERENCE_KIN": "Protein folding of the SAP domain, a naturally occurring two-helix bundle. FEBS Lett. 589(15):1740-7. https://doi.org/10.1016/j.febslet.2015.06.002", "YEAR_KIN": 2015.0, "PMID_KIN": "26073259", "STATE": "Yes", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Rd-apocyt b562 (de novo)", "SOURCE": "Escherichia coli", "LENGTH": 106.0, "UniProt": "P0ABE7", "PDB_wild": "1yyj", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I98V,N102A", "MUTATION_PDB": "I98V,N102A", "SEC_STR": "Helix,Helix", "RSA": "37.3,59.2", "T": 25.0, "pH": 5.0, "BUFFER_NAME": "NaAcetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.87, "ln(ku)_H2O": -3.91, "dGKIN_H2O": 21.77, "ddGKIN_H2O": -0.35, "mf": -3.14, "mu": 4.02, "BACKGROUND_MUT_UNIPROT": [ "M7W", "R98I", "N99R", "H102N", "R106G" ], "AUTHOR_KIN": "Chu R, Pei W, Takei J, Bai Y.", "REFERENCE_KIN": "Relationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein. Biochemistry. 41(25):7998-8003. doi: 10.1021/bi025872n", "YEAR_KIN": 2002.0, "PMID_KIN": "12069590", "STATE": "2", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Rd-apocyt b562 (de novo)", "SOURCE": "Escherichia coli", "LENGTH": 106.0, "UniProt": "P0ABE7", "PDB_wild": "1yyj", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I98V,N102A", "MUTATION_PDB": "I98V,N102A", "SEC_STR": "Helix,Helix", "RSA": "37.3,59.2", "T": 25.0, "pH": 5.0, "BUFFER_NAME": "NaAcetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.87, "ln(ku)_H2O": -3.91, "dGKIN_H2O": 21.77, "ddGKIN_H2O": -0.35, "mf": -3.14, "mu": 4.02, "BACKGROUND_MUT_UNIPROT": [ "M7W", "R98I", "N99R", "H102N", "R106G" ], "AUTHOR_KIN": "Chu R, Pei W, Takei J, Bai Y.", "REFERENCE_KIN": "Relationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein. Biochemistry. 41(25):7998-8003. doi: 10.1021/bi025872n", "YEAR_KIN": 2002.0, "PMID_KIN": "12069590", "STATE": "2", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Rd-apocyt b562 (de novo)", "SOURCE": "Escherichia coli", "LENGTH": 106.0, "UniProt": "P0ABE7", "PDB_wild": "1yyj", "MUTATED_CHAIN": "A", "MUTATION_UNIPROT": "I98V,N102G", "MUTATION_PDB": "I98V,N102G", "SEC_STR": "Helix,Helix", "RSA": "37.3,59.2", "T": 25.0, "pH": 5.0, "BUFFER_NAME": "NaAcetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.54, "ln(ku)_H2O": -1.43, "dGKIN_H2O": 14.79, "ddGKIN_H2O": -7.33, "mf": -3.26, "mu": 3.43, "PhiF_H2O": 0.16, "BACKGROUND_MUT_UNIPROT": [ "M7W", "R98I", "N99R", "H102N", "R106G" ], "AUTHOR_KIN": "Chu R, Pei W, Takei J, Bai Y.", "REFERENCE_KIN": "Relationship between the native-state hydrogen exchange and folding pathways of a four-helix bundle protein. 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J Mol Biol 320(2):389-402. https://doi.org/10.1016/S0022-2836(02)00445-X", "YEAR_KIN": 2002.0, "PMID_KIN": "12079394", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 62.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "T127A,S124K", "MUTATION_PDB": "T127A,S124K", "SEC_STR": "Coil,Beta", "RSA": "56.3,0.8", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris-HCl", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "mf": -4.58, "mu": 1.64, "beta-T": 0.74, "AUTHOR_KIN": "Northey JGB, Maxwell KL, Davidson AR", "REFERENCE_KIN": "Protein folding kinetics beyond the phi value: using multiple amino acid substitutions to investigate the structure of the SH3 domain folding transition state. J Mol Biol 320(2):389-402. https://doi.org/10.1016/S0022-2836(02)00445-X", "YEAR_KIN": 2002.0, "PMID_KIN": "12079394", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 62.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "G128A,S124K", "MUTATION_PDB": "G128A,S124K", "SEC_STR": "Coil,Beta", "RSA": "31.0,0.8", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris-HCl", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "mf": -6.62, "mu": 1.59, "beta-T": 0.81, "AUTHOR_KIN": "Northey JGB, Maxwell KL, Davidson AR", "REFERENCE_KIN": "Protein folding kinetics beyond the phi value: using multiple amino acid substitutions to investigate the structure of the SH3 domain folding transition state. J Mol Biol 320(2):389-402. https://doi.org/10.1016/S0022-2836(02)00445-X", "YEAR_KIN": 2002.0, "PMID_KIN": "12079394", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 62.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "G128A,S124K", "MUTATION_PDB": "G128A,S124K", "SEC_STR": "Coil,Beta", "RSA": "31.0,0.8", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris-HCl", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "mf": -6.62, "mu": 1.59, "beta-T": 0.81, "AUTHOR_KIN": "Northey JGB, Maxwell KL, Davidson AR", "REFERENCE_KIN": "Protein folding kinetics beyond the phi value: using multiple amino acid substitutions to investigate the structure of the SH3 domain folding transition state. J Mol Biol 320(2):389-402. https://doi.org/10.1016/S0022-2836(02)00445-X", "YEAR_KIN": 2002.0, "PMID_KIN": "12079394", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 59.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "A89L,F103L", "MUTATION_PDB": "A89L,F103L", "SEC_STR": "Beta,Beta", "RSA": "2.8,2.0", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 3.95, "dln(kf)_H2O": 0.54, "ln(ku)_H2O": 4.52, "dln(ku)_H2O": 5.19, "ddGKIN_H2O": -11.55, "mf": -6.28, "mu": 2.72, "PhiF_H2O": -0.11, "AUTHOR_KIN": "Northey, J.G.B., Di Nardo, A.A., Davidson, A.R.", "REFERENCE_KIN": "Hydrophobic core packing in the SH3 domain folding transition state. Nat. Struct. Biol. 9, 126\u2013130. https://doi.org/10.1038/nsb755", "YEAR_KIN": 2002.0, "PMID_KIN": "11786916", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 59.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "A89L,F103L", "MUTATION_PDB": "A89L,F103L", "SEC_STR": "Beta,Beta", "RSA": "2.8,2.0", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 3.95, "dln(kf)_H2O": 0.54, "ln(ku)_H2O": 4.52, "dln(ku)_H2O": 5.19, "ddGKIN_H2O": -11.55, "mf": -6.28, "mu": 2.72, "PhiF_H2O": -0.11, "AUTHOR_KIN": "Northey, J.G.B., Di Nardo, A.A., Davidson, A.R.", "REFERENCE_KIN": "Hydrophobic core packing in the SH3 domain folding transition state. Nat. Struct. Biol. 9, 126\u2013130. https://doi.org/10.1038/nsb755", "YEAR_KIN": 2002.0, "PMID_KIN": "11786916", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 59.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "A122G,V138I", "MUTATION_PDB": "A122G,V138I", "SEC_STR": "Beta,Beta", "RSA": "0.0,9.2", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 1.63, "dln(kf)_H2O": -1.78, "ln(ku)_H2O": -0.4, "dln(ku)_H2O": 0.27, "ddGKIN_H2O": -5.06, "mf": -8.95, "mu": 3.43, "AUTHOR_KIN": "Northey, J.G.B., Di Nardo, A.A., Davidson, A.R.", "REFERENCE_KIN": "Hydrophobic core packing in the SH3 domain folding transition state. Nat. Struct. 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Biol. 9, 126\u2013130. https://doi.org/10.1038/nsb785", "YEAR_KIN": 2002.0, "PMID_KIN": "11786916", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Fyn SH3", "SOURCE": "Homo sapiens", "LENGTH": 59.0, "UniProt": "P06241", "PDB_wild": "1shf", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shfA00 (2.30.30.40)" ], "EC_NUMBER": "2.7.10.2", "MUTATION_UNIPROT": "A122G,V138L", "MUTATION_PDB": "A122G,V138L", "SEC_STR": "Beta,Beta", "RSA": "0.0,9.2", "T": 25.0, "pH": 8.0, "BUFFER_NAME": "Tris", "BUFFER_CONC": "0.01", "ADDITIVES": "0.2 mM EDTA", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 1.72, "dln(kf)_H2O": -1.69, "ln(ku)_H2O": -0.78, "dln(ku)_H2O": -0.11, "ddGKIN_H2O": -3.93, "mf": -9.12, "mu": 3.1, "AUTHOR_KIN": "Northey, J.G.B., Di Nardo, A.A., Davidson, A.R.", "REFERENCE_KIN": "Hydrophobic core packing in the SH3 domain folding transition state. Nat. Struct. 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Proc Natl Acad Sci U S A 105(29): 9999\u201310004. https://doi.org/10.1073/pnas.0801874105", "YEAR_KIN": 2008.0, "PMID_KIN": "18626019", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L971S,D1011G", "MUTATION_PDB": "L8S,D48G", "SEC_STR": "Beta,Coil", "RSA": "42.1,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.08, "dln(kf)_H2O": 2.77, "ln(ku)_H2O": -3.24, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 18.14, "ddGKIN_H2O": -3.8, "mf": -2.06, "mu": 1.06, "PhiF_H2O": 0.16, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14898", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L971S,D1011G", "MUTATION_PDB": "L8S,D48G", "SEC_STR": "Beta,Coil", "RSA": "42.1,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.08, "dln(kf)_H2O": 2.77, "ln(ku)_H2O": -3.24, "dln(ku)_H2O": 2.06, "dGKIN_H2O": 18.14, "ddGKIN_H2O": -3.8, "mf": -2.06, "mu": 1.06, "PhiF_H2O": 0.16, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14898", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A974G,D1011G", "MUTATION_PDB": "A11G,D48G", "SEC_STR": "Beta,Coil", "RSA": "1.9,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.26, "dln(kf)_H2O": 2.95, "ln(ku)_H2O": -0.63, "dln(ku)_H2O": 4.67, "dGKIN_H2O": 12.13, "ddGKIN_H2O": -9.81, "mf": -2.3, "mu": 0.97, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14899", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A974G,D1011G", "MUTATION_PDB": "A11G,D48G", "SEC_STR": "Beta,Coil", "RSA": "1.9,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.26, "dln(kf)_H2O": 2.95, "ln(ku)_H2O": -0.63, "dln(ku)_H2O": 4.67, "dGKIN_H2O": 12.13, "ddGKIN_H2O": -9.81, "mf": -2.3, "mu": 0.97, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14899", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V986A,D1011G", "MUTATION_PDB": "V23A,D48G", "SEC_STR": "Coil,Coil", "RSA": "1.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.18, "dln(kf)_H2O": 1.87, "ln(ku)_H2O": -2.3, "dln(ku)_H2O": 3.0, "dGKIN_H2O": 13.58, "ddGKIN_H2O": -8.36, "mf": -1.88, "mu": 1.29, "PhiF_H2O": 0.32, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14901", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V986A,D1011G", "MUTATION_PDB": "V23A,D48G", "SEC_STR": "Coil,Coil", "RSA": "1.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.18, "dln(kf)_H2O": 1.87, "ln(ku)_H2O": -2.3, "dln(ku)_H2O": 3.0, "dGKIN_H2O": 13.58, "ddGKIN_H2O": -8.36, "mf": -1.88, "mu": 1.29, "PhiF_H2O": 0.32, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14901", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "T987A,D1011G", "MUTATION_PDB": "T24A,D48G", "SEC_STR": "Coil,Coil", "RSA": "39.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.81, "dln(kf)_H2O": 2.5, "ln(ku)_H2O": -3.22, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 17.41, "ddGKIN_H2O": -4.53, "mf": -2.06, "mu": 1.06, "PhiF_H2O": 0.29, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14902", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "T987A,D1011G", "MUTATION_PDB": "T24A,D48G", "SEC_STR": "Coil,Coil", "RSA": "39.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.81, "dln(kf)_H2O": 2.5, "ln(ku)_H2O": -3.22, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 17.41, "ddGKIN_H2O": -4.53, "mf": -2.06, "mu": 1.06, "PhiF_H2O": 0.29, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14902", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "D992A,D1011G", "MUTATION_PDB": "D29A,D48G", "SEC_STR": "Coil,Coil", "RSA": "33.1,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.89, "dln(kf)_H2O": 2.58, "ln(ku)_H2O": -3.0, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 17.06, "ddGKIN_H2O": -4.88, "mf": -2.28, "mu": 1.14, "PhiF_H2O": 0.22, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14903", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "D992A,D1011G", "MUTATION_PDB": "D29A,D48G", "SEC_STR": "Coil,Coil", "RSA": "33.1,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.89, "dln(kf)_H2O": 2.58, "ln(ku)_H2O": -3.0, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 17.06, "ddGKIN_H2O": -4.88, "mf": -2.28, "mu": 1.14, "PhiF_H2O": 0.22, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14903", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L996V,D1011G", "MUTATION_PDB": "L33V,D48G", "SEC_STR": "Beta,Coil", "RSA": "6.1,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.85, "dln(kf)_H2O": 3.54, "ln(ku)_H2O": -2.21, "dln(ku)_H2O": 3.09, "dGKIN_H2O": 17.49, "ddGKIN_H2O": -4.45, "mf": -1.93, "mu": 1.16, "PhiF_H2O": -0.2, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. 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Biol. 6, 1010\u20131016. https://doi.org/10.1038/14904", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "S999N,D1011G", "MUTATION_PDB": "S36N,D48G", "SEC_STR": "Coil,Coil", "RSA": "34.6,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.87, "dln(kf)_H2O": 2.56, "ln(ku)_H2O": -3.22, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 17.57, "ddGKIN_H2O": -4.37, "mf": -2.28, "mu": 0.89, "PhiF_H2O": 0.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. 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Biol. 6, 1010\u20131016. https://doi.org/10.1038/14905", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "K1006A,D1011G", "MUTATION_PDB": "K43A,D48G", "SEC_STR": "Beta,Coil", "RSA": "31.2,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.91, "dln(kf)_H2O": 2.6, "ln(ku)_H2O": -3.32, "dln(ku)_H2O": 1.98, "dGKIN_H2O": 17.93, "ddGKIN_H2O": -4.01, "mf": -2.13, "mu": 1.06, "PhiF_H2O": 0.26, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14906", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "K1006A,D1011G", "MUTATION_PDB": "K43A,D48G", "SEC_STR": "Beta,Coil", "RSA": "31.2,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.91, "dln(kf)_H2O": 2.6, "ln(ku)_H2O": -3.32, "dln(ku)_H2O": 1.98, "dGKIN_H2O": 17.93, "ddGKIN_H2O": -4.01, "mf": -2.13, "mu": 1.06, "PhiF_H2O": 0.26, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14906", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1007A,D1011G", "MUTATION_PDB": "V44A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.61, "dln(kf)_H2O": 0.3, "ln(ku)_H2O": -1.51, "dln(ku)_H2O": 3.79, "dGKIN_H2O": 7.74, "ddGKIN_H2O": -14.2, "mf": -2.63, "mu": 1.04, "PhiF_H2O": 0.48, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14907", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1007A,D1011G", "MUTATION_PDB": "V44A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.61, "dln(kf)_H2O": 0.3, "ln(ku)_H2O": -1.51, "dln(ku)_H2O": 3.79, "dGKIN_H2O": 7.74, "ddGKIN_H2O": -14.2, "mf": -2.63, "mu": 1.04, "PhiF_H2O": 0.48, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14907", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1009A,D1011G", "MUTATION_PDB": "V46A,D48G", "SEC_STR": "Beta,Coil", "RSA": "12.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.42, "dln(kf)_H2O": 1.11, "ln(ku)_H2O": -4.96, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 18.3, "ddGKIN_H2O": -3.64, "mf": -2.21, "mu": 1.06, "PhiF_H2O": 1.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14908", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1009A,D1011G", "MUTATION_PDB": "V46A,D48G", "SEC_STR": "Beta,Coil", "RSA": "12.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.42, "dln(kf)_H2O": 1.11, "ln(ku)_H2O": -4.96, "dln(ku)_H2O": 0.34, "dGKIN_H2O": 18.3, "ddGKIN_H2O": -3.64, "mf": -2.21, "mu": 1.06, "PhiF_H2O": 1.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14908", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "F1015A,D1011G", "MUTATION_PDB": "F52A,D48G", "SEC_STR": "Beta,Coil", "RSA": "28.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.7, "dln(kf)_H2O": 0.39, "ln(ku)_H2O": -2.66, "dln(ku)_H2O": 2.64, "dGKIN_H2O": 10.81, "ddGKIN_H2O": -11.13, "mf": -2.48, "mu": 0.84, "PhiF_H2O": 0.58, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14910", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "F1015A,D1011G", "MUTATION_PDB": "F52A,D48G", "SEC_STR": "Beta,Coil", "RSA": "28.4,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.7, "dln(kf)_H2O": 0.39, "ln(ku)_H2O": -2.66, "dln(ku)_H2O": 2.64, "dGKIN_H2O": 10.81, "ddGKIN_H2O": -11.13, "mf": -2.48, "mu": 0.84, "PhiF_H2O": 0.58, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14910", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1016A,D1011G", "MUTATION_PDB": "V53A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.08, "dln(kf)_H2O": 0.77, "ln(ku)_H2O": -3.22, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 13.13, "ddGKIN_H2O": -8.81, "mf": -2.3, "mu": 1.19, "PhiF_H2O": 0.61, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14911", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1016A,D1011G", "MUTATION_PDB": "V53A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.08, "dln(kf)_H2O": 0.77, "ln(ku)_H2O": -3.22, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 13.13, "ddGKIN_H2O": -8.81, "mf": -2.3, "mu": 1.19, "PhiF_H2O": 0.61, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14911", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A1018G,D1011G", "MUTATION_PDB": "A55G,D48G", "SEC_STR": "Helix,Coil", "RSA": "13.2,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.56, "dln(kf)_H2O": 1.25, "ln(ku)_H2O": -3.0, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 13.76, "ddGKIN_H2O": -8.18, "mf": -2.38, "mu": 1.04, "PhiF_H2O": 0.53, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14912", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A1018G,D1011G", "MUTATION_PDB": "A55G,D48G", "SEC_STR": "Helix,Coil", "RSA": "13.2,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.56, "dln(kf)_H2O": 1.25, "ln(ku)_H2O": -3.0, "dln(ku)_H2O": 2.3, "dGKIN_H2O": 13.76, "ddGKIN_H2O": -8.18, "mf": -2.38, "mu": 1.04, "PhiF_H2O": 0.53, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14912", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1021A,D1011G", "MUTATION_PDB": "V58A,D48G", "SEC_STR": "Beta,Coil", "RSA": "8.5,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.71, "dln(kf)_H2O": 2.4, "ln(ku)_H2O": -1.14, "dln(ku)_H2O": 4.16, "dGKIN_H2O": 12.02, "ddGKIN_H2O": -9.92, "mf": -2.38, "mu": 1.11, "PhiF_H2O": 0.16, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14913", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1021A,D1011G", "MUTATION_PDB": "V58A,D48G", "SEC_STR": "Beta,Coil", "RSA": "8.5,69.3", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Phosphate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.71, "dln(kf)_H2O": 2.4, "ln(ku)_H2O": -1.14, "dln(ku)_H2O": 4.16, "dGKIN_H2O": 12.02, "ddGKIN_H2O": -9.92, "mf": -2.38, "mu": 1.11, "PhiF_H2O": 0.16, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14913", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L971S,D1011G", "MUTATION_PDB": "L8S,D48G", "SEC_STR": "Beta,Coil", "RSA": "42.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.81, "dln(kf)_H2O": 1.29, "ln(ku)_H2O": -1.27, "dln(ku)_H2O": 1.26, "dGKIN_H2O": 12.59, "ddGKIN_H2O": -3.36, "mf": -3.39, "mu": 1.19, "PhiF_H2O": 0.07, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14934", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L971S,D1011G", "MUTATION_PDB": "L8S,D48G", "SEC_STR": "Beta,Coil", "RSA": "42.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.81, "dln(kf)_H2O": 1.29, "ln(ku)_H2O": -1.27, "dln(ku)_H2O": 1.26, "dGKIN_H2O": 12.59, "ddGKIN_H2O": -3.36, "mf": -3.39, "mu": 1.19, "PhiF_H2O": 0.07, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14934", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A974G,D1011G", "MUTATION_PDB": "A11G,D48G", "SEC_STR": "Beta,Coil", "RSA": "1.9,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.76, "dln(kf)_H2O": 1.24, "ln(ku)_H2O": 0.34, "dln(ku)_H2O": 2.87, "dGKIN_H2O": 8.47, "ddGKIN_H2O": -7.48, "mf": -3.12, "mu": 1.26, "PhiF_H2O": 0.05, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14935", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A974G,D1011G", "MUTATION_PDB": "A11G,D48G", "SEC_STR": "Beta,Coil", "RSA": "1.9,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.76, "dln(kf)_H2O": 1.24, "ln(ku)_H2O": 0.34, "dln(ku)_H2O": 2.87, "dGKIN_H2O": 8.47, "ddGKIN_H2O": -7.48, "mf": -3.12, "mu": 1.26, "PhiF_H2O": 0.05, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14935", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V986A,D1011G", "MUTATION_PDB": "V23A,D48G", "SEC_STR": "Coil,Coil", "RSA": "1.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.18, "dln(kf)_H2O": 0.66, "ln(ku)_H2O": -0.51, "dln(ku)_H2O": 2.02, "dGKIN_H2O": 9.14, "ddGKIN_H2O": -6.81, "mf": -3.42, "mu": 1.36, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14937", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V986A,D1011G", "MUTATION_PDB": "V23A,D48G", "SEC_STR": "Coil,Coil", "RSA": "1.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.18, "dln(kf)_H2O": 0.66, "ln(ku)_H2O": -0.51, "dln(ku)_H2O": 2.02, "dGKIN_H2O": 9.14, "ddGKIN_H2O": -6.81, "mf": -3.42, "mu": 1.36, "PhiF_H2O": 0.27, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14937", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "T987A,D1011G", "MUTATION_PDB": "T24A,D48G", "SEC_STR": "Coil,Coil", "RSA": "39.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.5, "dln(kf)_H2O": 0.98, "ln(ku)_H2O": -1.24, "dln(ku)_H2O": 1.29, "dGKIN_H2O": 11.73, "ddGKIN_H2O": -4.22, "mf": -2.82, "mu": 1.24, "PhiF_H2O": 0.24, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14938", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "T987A,D1011G", "MUTATION_PDB": "T24A,D48G", "SEC_STR": "Coil,Coil", "RSA": "39.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.5, "dln(kf)_H2O": 0.98, "ln(ku)_H2O": -1.24, "dln(ku)_H2O": 1.29, "dGKIN_H2O": 11.73, "ddGKIN_H2O": -4.22, "mf": -2.82, "mu": 1.24, "PhiF_H2O": 0.24, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14938", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "D992A,D1011G", "MUTATION_PDB": "D29A,D48G", "SEC_STR": "Coil,Coil", "RSA": "33.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.61, "dln(kf)_H2O": 1.09, "ln(ku)_H2O": -1.66, "dln(ku)_H2O": 0.87, "dGKIN_H2O": 13.06, "ddGKIN_H2O": -2.89, "mf": -3.12, "mu": 1.54, "PhiF_H2O": 0.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14939", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "D992A,D1011G", "MUTATION_PDB": "D29A,D48G", "SEC_STR": "Coil,Coil", "RSA": "33.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.61, "dln(kf)_H2O": 1.09, "ln(ku)_H2O": -1.66, "dln(ku)_H2O": 0.87, "dGKIN_H2O": 13.06, "ddGKIN_H2O": -2.89, "mf": -3.12, "mu": 1.54, "PhiF_H2O": 0.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14939", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L996V,D1011G", "MUTATION_PDB": "L33V,D48G", "SEC_STR": "Beta,Coil", "RSA": "6.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.44, "dln(kf)_H2O": 1.92, "ln(ku)_H2O": -0.45, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 12.11, "ddGKIN_H2O": -3.84, "mf": -3.1, "mu": 1.21, "PhiF_H2O": -0.3, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14940", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "L996V,D1011G", "MUTATION_PDB": "L33V,D48G", "SEC_STR": "Beta,Coil", "RSA": "6.1,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 4.44, "dln(kf)_H2O": 1.92, "ln(ku)_H2O": -0.45, "dln(ku)_H2O": 2.08, "dGKIN_H2O": 12.11, "ddGKIN_H2O": -3.84, "mf": -3.1, "mu": 1.21, "PhiF_H2O": -0.3, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14940", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "S999N,D1011G", "MUTATION_PDB": "S36N,D48G", "SEC_STR": "Coil,Coil", "RSA": "34.6,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.78, "dln(kf)_H2O": 1.26, "ln(ku)_H2O": -1.61, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 13.36, "ddGKIN_H2O": -2.59, "mf": -2.92, "mu": 1.24, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14941", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "S999N,D1011G", "MUTATION_PDB": "S36N,D48G", "SEC_STR": "Coil,Coil", "RSA": "34.6,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.78, "dln(kf)_H2O": 1.26, "ln(ku)_H2O": -1.61, "dln(ku)_H2O": 0.92, "dGKIN_H2O": 13.36, "ddGKIN_H2O": -2.59, "mf": -2.92, "mu": 1.24, "PhiF_H2O": 0.12, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14941", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "K1006A,D1011G", "MUTATION_PDB": "K43A,D48G", "SEC_STR": "Beta,Coil", "RSA": "31.2,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.64, "dln(kf)_H2O": 1.12, "ln(ku)_H2O": -1.71, "dln(ku)_H2O": 0.82, "dGKIN_H2O": 13.26, "ddGKIN_H2O": -2.69, "mf": -2.8, "mu": 1.31, "PhiF_H2O": 0.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14942", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "K1006A,D1011G", "MUTATION_PDB": "K43A,D48G", "SEC_STR": "Beta,Coil", "RSA": "31.2,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.64, "dln(kf)_H2O": 1.12, "ln(ku)_H2O": -1.71, "dln(ku)_H2O": 0.82, "dGKIN_H2O": 13.26, "ddGKIN_H2O": -2.69, "mf": -2.8, "mu": 1.31, "PhiF_H2O": 0.25, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14942", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1007A,D1011G", "MUTATION_PDB": "V44A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.1, "dln(kf)_H2O": -1.42, "ln(ku)_H2O": 0.12, "dln(ku)_H2O": 2.65, "dGKIN_H2O": 2.42, "ddGKIN_H2O": -13.53, "mf": -2.97, "mu": 1.24, "PhiF_H2O": 0.52, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14943", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1007A,D1011G", "MUTATION_PDB": "V44A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.1, "dln(kf)_H2O": -1.42, "ln(ku)_H2O": 0.12, "dln(ku)_H2O": 2.65, "dGKIN_H2O": 2.42, "ddGKIN_H2O": -13.53, "mf": -2.97, "mu": 1.24, "PhiF_H2O": 0.52, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14943", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1009A,D1011G", "MUTATION_PDB": "V46A,D48G", "SEC_STR": "Beta,Coil", "RSA": "12.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.17, "dln(kf)_H2O": -0.35, "ln(ku)_H2O": -2.66, "dln(ku)_H2O": -0.13, "dGKIN_H2O": 11.98, "ddGKIN_H2O": -3.97, "mf": -3.0, "mu": 1.07, "PhiF_H2O": 1.1, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14944", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1009A,D1011G", "MUTATION_PDB": "V46A,D48G", "SEC_STR": "Beta,Coil", "RSA": "12.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 2.17, "dln(kf)_H2O": -0.35, "ln(ku)_H2O": -2.66, "dln(ku)_H2O": -0.13, "dGKIN_H2O": 11.98, "ddGKIN_H2O": -3.97, "mf": -3.0, "mu": 1.07, "PhiF_H2O": 1.1, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14944", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "F1015A,D1011G", "MUTATION_PDB": "F52A,D48G", "SEC_STR": "Beta,Coil", "RSA": "28.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.16, "dln(kf)_H2O": -1.36, "ln(ku)_H2O": -0.97, "dln(ku)_H2O": 1.56, "dGKIN_H2O": 5.28, "ddGKIN_H2O": -10.67, "mf": -2.97, "mu": 1.14, "PhiF_H2O": 0.64, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14946", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "F1015A,D1011G", "MUTATION_PDB": "F52A,D48G", "SEC_STR": "Beta,Coil", "RSA": "28.4,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.16, "dln(kf)_H2O": -1.36, "ln(ku)_H2O": -0.97, "dln(ku)_H2O": 1.56, "dGKIN_H2O": 5.28, "ddGKIN_H2O": -10.67, "mf": -2.97, "mu": 1.14, "PhiF_H2O": 0.64, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14946", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1016A,D1011G", "MUTATION_PDB": "V53A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 0.96, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": -0.73, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 4.19, "ddGKIN_H2O": -11.76, "mf": -2.97, "mu": 1.26, "PhiF_H2O": 0.62, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14947", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1016A,D1011G", "MUTATION_PDB": "V53A,D48G", "SEC_STR": "Beta,Coil", "RSA": "0.0,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 0.96, "dln(kf)_H2O": -1.56, "ln(ku)_H2O": -0.73, "dln(ku)_H2O": 1.8, "dGKIN_H2O": 4.19, "ddGKIN_H2O": -11.76, "mf": -2.97, "mu": 1.26, "PhiF_H2O": 0.62, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14947", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A1018G,D1011G", "MUTATION_PDB": "A55G,D48G", "SEC_STR": "Helix,Coil", "RSA": "13.2,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.59, "dln(kf)_H2O": -0.93, "ln(ku)_H2O": -1.43, "dln(ku)_H2O": 1.1, "dGKIN_H2O": 7.47, "ddGKIN_H2O": -8.48, "mf": -2.6, "mu": 1.31, "PhiF_H2O": 0.68, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14948", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "A1018G,D1011G", "MUTATION_PDB": "A55G,D48G", "SEC_STR": "Helix,Coil", "RSA": "13.2,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 1.59, "dln(kf)_H2O": -0.93, "ln(ku)_H2O": -1.43, "dln(ku)_H2O": 1.1, "dGKIN_H2O": 7.47, "ddGKIN_H2O": -8.48, "mf": -2.6, "mu": 1.31, "PhiF_H2O": 0.68, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14948", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1021A,D1011G", "MUTATION_PDB": "V58A,D48G", "SEC_STR": "Beta,Coil", "RSA": "8.5,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.14, "dln(kf)_H2O": 0.62, "ln(ku)_H2O": 0.22, "dln(ku)_H2O": 2.75, "dGKIN_H2O": 7.24, "ddGKIN_H2O": -8.71, "mf": -3.07, "mu": 1.46, "PhiF_H2O": 0.22, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14949", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "Spectrin SH3", "SOURCE": "Gallus gallus", "LENGTH": 62.0, "UniProt": "P07751", "PDB_wild": "1shg", "MUTATED_CHAIN": "A", "PFAM": [ "PF00018" ], "CATH": [ "1shgA00 (2.30.30.40)" ], "MUTATION_UNIPROT": "V1021A,D1011G", "MUTATION_PDB": "V58A,D48G", "SEC_STR": "Beta,Coil", "RSA": "8.5,69.3", "T": 25.0, "pH": 3.5, "BUFFER_NAME": "Glycine", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "urea", "ln(kf)_H2O": 3.14, "dln(kf)_H2O": 0.62, "ln(ku)_H2O": 0.22, "dln(ku)_H2O": 2.75, "dGKIN_H2O": 7.24, "ddGKIN_H2O": -8.71, "mf": -3.07, "mu": 1.46, "PhiF_H2O": 0.22, "AUTHOR_KIN": "Mart\u00ednez, J.C., Serrano, L.", "REFERENCE_KIN": "The folding transition state between SH3 domains is conformationally restricted and evolutionarily conserved. Nat. Struct. Biol. 6, 1010\u20131016. https://doi.org/10.1038/14949", "YEAR_KIN": 1999.0, "PMID_KIN": "10542091", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "CI2", "SOURCE": "Hordeum vulgare", "LENGTH": 83.0, "UniProt": "P01053", "PDB_wild": "1coa", "MUTATED_CHAIN": "I", "PFAM": [ "PF00280" ], "CATH": [ "1coaI00 (3.30.10.10)" ], "MUTATION_UNIPROT": "V58A,F70L", "MUTATION_PDB": "V57A,F69L", "SEC_STR": "Coil,Beta", "RSA": "40.8,16.2", "T": 25.0, "pH": 6.25, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 2.9, "dln(kf)_H2O": -1.13, "ln(ku)_H2O": -6.31, "dln(ku)_H2O": 2.73, "dGKIN_H2O": 22.82, "mf": -6.39, "mu": 2.92, "AUTHOR_KIN": "Itzhaki LS, Otzen DE, Fersht AR", "REFERENCE_KIN": "The structure of the transition state for folding of chymotrypsin inhibitor 2 analysed by protein engineering methods: evidence for a nucleation-condensation mechanism for protein folding, J Mol Biol. 254(2):260-288. doi: 10.1006/jmbi.1995.0616.", "YEAR_KIN": 1995.0, "PMID_KIN": "7490748", "STATE": "2", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "CI2", "SOURCE": "Hordeum vulgare", "LENGTH": 83.0, "UniProt": "P01053", "PDB_wild": "1coa", "MUTATED_CHAIN": "I", "PFAM": [ "PF00280" ], "CATH": [ "1coaI00 (3.30.10.10)" ], "MUTATION_UNIPROT": "V58A,F70L", "MUTATION_PDB": "V57A,F69L", "SEC_STR": "Coil,Beta", "RSA": "40.8,16.2", "T": 25.0, "pH": 6.25, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 2.9, "dln(kf)_H2O": -1.13, "ln(ku)_H2O": -6.31, "dln(ku)_H2O": 2.73, "dGKIN_H2O": 22.82, "mf": -6.39, "mu": 2.92, "AUTHOR_KIN": "Itzhaki LS, Otzen DE, Fersht AR", "REFERENCE_KIN": "The structure of the transition state for folding of chymotrypsin inhibitor 2 analysed by protein engineering methods: evidence for a nucleation-condensation mechanism for protein folding, J Mol Biol. 254(2):260-288. doi: 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10.1006/jmbi.1995.0616.", "YEAR_KIN": 1995.0, "PMID_KIN": "7490748", "STATE": "2", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "CI2", "SOURCE": "Hordeum vulgare", "LENGTH": 83.0, "UniProt": "P01053", "PDB_wild": "1coa", "MUTATED_CHAIN": "I", "PFAM": [ "PF00280" ], "CATH": [ "1coaI00 (3.30.10.10)" ], "MUTATION_UNIPROT": "L52V,V58A", "MUTATION_PDB": "L51V,V57A", "SEC_STR": "Beta,Coil", "RSA": "23.8,40.8", "T": 25.0, "pH": 6.25, "BUFFER_NAME": "MES", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GuHCl", "ln(kf)_H2O": 3.82, "dln(kf)_H2O": -0.21, "ln(ku)_H2O": -6.44, "dln(ku)_H2O": 2.6, "dGKIN_H2O": 25.42, "mf": -6.02, "mu": 3.05, "AUTHOR_KIN": "Itzhaki LS, Otzen DE, Fersht AR", "REFERENCE_KIN": "The structure of the transition state for folding of chymotrypsin inhibitor 2 analysed by protein engineering methods: evidence for a nucleation-condensation mechanism for protein folding, J Mol Biol. 254(2):260-288. doi: 10.1006/jmbi.1995.0616.", "YEAR_KIN": 1995.0, "PMID_KIN": "7490748", "STATE": "2", "REVIEW_DATE": "2023-06-04T01:00:00" } ]