[ { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "dGKIN_H2O": 12.13, "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "WT", "MUTATION_PDB": "WT", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "dGKIN_H2O": 12.13, "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I400A", "MUTATION_PDB": "I3A", "SEC_STR": "Coil", "RSA": "28.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I400A", "MUTATION_PDB": "I3A", "SEC_STR": "Coil", "RSA": "28.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I400V", "MUTATION_PDB": "I3V", "SEC_STR": "Coil", "RSA": "28.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I400V", "MUTATION_PDB": "I3V", "SEC_STR": "Coil", "RSA": "28.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T401A", "MUTATION_PDB": "T4A", "SEC_STR": "Beta", "RSA": "57.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T401A", "MUTATION_PDB": "T4A", "SEC_STR": "Beta", "RSA": "57.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T401S", "MUTATION_PDB": "T4S", "SEC_STR": "Beta", "RSA": "57.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T401S", "MUTATION_PDB": "T4S", "SEC_STR": "Beta", "RSA": "57.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "Y402F", "MUTATION_PDB": "Y5F", "SEC_STR": "Beta", "RSA": "8.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "Y402F", "MUTATION_PDB": "Y5F", "SEC_STR": "Beta", "RSA": "8.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R403A", "MUTATION_PDB": "R6A", "SEC_STR": "Beta", "RSA": "58.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R403A", "MUTATION_PDB": "R6A", "SEC_STR": "Beta", "RSA": "58.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V404A", "MUTATION_PDB": "V7A", "SEC_STR": "Beta", "RSA": "4.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V404A", "MUTATION_PDB": "V7A", "SEC_STR": "Beta", "RSA": "4.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R405A", "MUTATION_PDB": "R8A", "SEC_STR": "Coil", "RSA": "63.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R405A", "MUTATION_PDB": "R8A", "SEC_STR": "Coil", "RSA": "63.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S409A", "MUTATION_PDB": "S12A", "SEC_STR": "Coil", "RSA": "50.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S409A", "MUTATION_PDB": "S12A", "SEC_STR": "Coil", "RSA": "50.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L410A", "MUTATION_PDB": "L13A", "SEC_STR": "Helix", "RSA": "26.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L410A", "MUTATION_PDB": "L13A", "SEC_STR": "Helix", "RSA": "26.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S411A", "MUTATION_PDB": "S14A", "SEC_STR": "Helix", "RSA": "63.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S411A", "MUTATION_PDB": "S14A", "SEC_STR": "Helix", "RSA": "63.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S411G", "MUTATION_PDB": "S14G", "SEC_STR": "Helix", "RSA": "63.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S411G", "MUTATION_PDB": "S14G", "SEC_STR": "Helix", "RSA": "63.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S412A", "MUTATION_PDB": "S15A", "SEC_STR": "Helix", "RSA": "49.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S412A", "MUTATION_PDB": "S15A", "SEC_STR": "Helix", "RSA": "49.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S412G", "MUTATION_PDB": "S15G", "SEC_STR": "Helix", "RSA": "49.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "S412G", "MUTATION_PDB": "S15G", "SEC_STR": "Helix", "RSA": "49.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I413V", "MUTATION_PDB": "I16V", "SEC_STR": "Helix", "RSA": "3.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I413V", "MUTATION_PDB": "I16V", "SEC_STR": "Helix", "RSA": "3.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "A414G", "MUTATION_PDB": "A17G", "SEC_STR": "Helix", "RSA": "3.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "A414G", "MUTATION_PDB": "A17G", "SEC_STR": "Helix", "RSA": "3.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K415A", "MUTATION_PDB": "K18A", "SEC_STR": "Helix", "RSA": "84.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K415A", "MUTATION_PDB": "K18A", "SEC_STR": "Helix", "RSA": "84.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K415G", "MUTATION_PDB": "K18G", "SEC_STR": "Helix", "RSA": "84.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K415G", "MUTATION_PDB": "K18G", "SEC_STR": "Helix", "RSA": "84.9", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R416A", "MUTATION_PDB": "R19A", "SEC_STR": "Helix", "RSA": "87.5", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R416A", "MUTATION_PDB": "R19A", "SEC_STR": "Helix", "RSA": "87.5", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "H417A", "MUTATION_PDB": "H20A", "SEC_STR": "Helix", "RSA": "30.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "H417A", "MUTATION_PDB": "H20A", "SEC_STR": "Helix", "RSA": "30.4", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "G418A", "MUTATION_PDB": "G21A", "SEC_STR": "Coil", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "G418A", "MUTATION_PDB": "G21A", "SEC_STR": "Coil", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V419A", "MUTATION_PDB": "V22A", "SEC_STR": "Coil", "RSA": "10.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V419A", "MUTATION_PDB": "V22A", "SEC_STR": "Coil", "RSA": "10.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I421A", "MUTATION_PDB": "I24A", "SEC_STR": "Helix", "RSA": "10.1", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I421A", "MUTATION_PDB": "I24A", "SEC_STR": "Helix", "RSA": "10.1", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I421V", "MUTATION_PDB": "I24V", "SEC_STR": "Helix", "RSA": "10.1", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "I421V", "MUTATION_PDB": "I24V", "SEC_STR": "Helix", "RSA": "10.1", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K422A", "MUTATION_PDB": "K25A", "SEC_STR": "Helix", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K422A", "MUTATION_PDB": "K25A", "SEC_STR": "Helix", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K422G", "MUTATION_PDB": "K25G", "SEC_STR": "Helix", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "K422G", "MUTATION_PDB": "K25G", "SEC_STR": "Helix", "RSA": "70.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "D423A", "MUTATION_PDB": "D26A", "SEC_STR": "Helix", "RSA": "22.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "D423A", "MUTATION_PDB": "D26A", "SEC_STR": "Helix", "RSA": "22.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V424A", "MUTATION_PDB": "V27A", "SEC_STR": "Helix", "RSA": "0.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "V424A", "MUTATION_PDB": "V27A", "SEC_STR": "Helix", "RSA": "0.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "M425A", "MUTATION_PDB": "M28A", "SEC_STR": "Helix", "RSA": "58.5", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "M425A", "MUTATION_PDB": "M28A", "SEC_STR": "Helix", "RSA": "58.5", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R426A", "MUTATION_PDB": "R29A", "SEC_STR": "Helix", "RSA": "76.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R426A", "MUTATION_PDB": "R29A", "SEC_STR": "Helix", "RSA": "76.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R426G", "MUTATION_PDB": "R29G", "SEC_STR": "Helix", "RSA": "76.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "R426G", "MUTATION_PDB": "R29G", "SEC_STR": "Helix", "RSA": "76.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T431S", "MUTATION_PDB": "T34S", "SEC_STR": "Helix", "RSA": "29.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T431S", "MUTATION_PDB": "T34S", "SEC_STR": "Helix", "RSA": "29.6", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L434A", "MUTATION_PDB": "L37A", "SEC_STR": "Coil", "RSA": "23.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L434A", "MUTATION_PDB": "L37A", "SEC_STR": "Coil", "RSA": "23.8", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "D438A", "MUTATION_PDB": "D41A", "SEC_STR": "Beta", "RSA": "30.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "D438A", "MUTATION_PDB": "D41A", "SEC_STR": "Beta", "RSA": "30.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L440M", "MUTATION_PDB": "L43M", "SEC_STR": "Beta", "RSA": "4.3", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L440M", "MUTATION_PDB": "L43M", "SEC_STR": "Beta", "RSA": "4.3", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T441A", "MUTATION_PDB": "T44A", "SEC_STR": "Beta", "RSA": "28.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T441A", "MUTATION_PDB": "T44A", "SEC_STR": "Beta", "RSA": "28.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T441S", "MUTATION_PDB": "T44S", "SEC_STR": "Beta", "RSA": "28.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "T441S", "MUTATION_PDB": "T44S", "SEC_STR": "Beta", "RSA": "28.2", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.44, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L442M", "MUTATION_PDB": "L45M", "SEC_STR": "Coil", "RSA": "0.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "L442M", "MUTATION_PDB": "L45M", "SEC_STR": "Coil", "RSA": "0.0", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 4.6, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "F443L", "MUTATION_PDB": "F46L", "SEC_STR": "Coil", "RSA": "47.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" }, { "PROTEIN": "LysM domain of Membrane-bound lytic murein transglycosylase D", "SOURCE": "Escherichia coli", "LENGTH": 48.0, "UniProt": "P0AEZ7", "PDB_wild": "1e0g", "MUTATED_CHAIN": "A", "PFAM": [ "PF01476" ], "CATH": [ "1e0gA00 (3.10.350.10)" ], "EC_NUMBER": "4.2.2.n1", "MUTATION_UNIPROT": "F443L", "MUTATION_PDB": "F46L", "SEC_STR": "Coil", "RSA": "47.7", "T": 11.0, "pH": 7.0, "BUFFER_NAME": "MOPS", "BUFFER_CONC": "0.05", "MEASURE_KIN": "stopped-flow", "METHOD_KIN": "GdmCl", "mu": 5.02, "AUTHOR_KIN": "Nickson AA, Stoll KE, Clarke J", "REFERENCE_KIN": "Folding of a LysM domain: entropy-enthalpy compensation in the transition state of an ideal two-state folder. J Mol Bio 380(3):557-69. https://doi.org/10.1016/j.jmb.2008.05.020", "YEAR_KIN": 2008.0, "PMID_KIN": "18538343", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-11T01:00:00" } ]