[
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "WT(F653W)",
      "MUTATION_PDB": "WT(F45W)",
      "T": 25.0,
      "pH": 5.0,
      "BUFFER_NAME": "Acetate",
      "BUFFER_CONC": "0.02",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 7.33,
      "ln(ku)_H2O": -6.84,
      "dGKIN_H2O": 35.11,
      "mf": -5.66,
      "mu": 3.43,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Maxwell, K.L., Wildes, D., Zarrine-Afsar, A., De Los Rios, M.A., Brown, A.G., Friel, C.T., Hedberg, L., Horng, J.-C., Bona, D., Miller, E.J., Vall\u00e9e-B\u00e9lisle, A., Main, E.R.G., Bemporad, F., Qiu, L., Teilum, K., Vu, N.-D., Edwards, A.M., Ruczinski, I., Poulsen, F.M., Kragelund, B.B., Michnick, S.W., Chiti, F., Bai, Y., Hagen, S.J., Serrano, L., Oliveberg, M., Raleigh, D.P., Wittung-Stafshede, P., Radford, S.E., Jackson, S.E., Sosnick, T.R., Marqusee, S., Davidson, A.R., Plaxco, K.W.",
      "REFERENCE_KIN": "Protein folding: defining a \u201cstandard\u201d set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci. 14, 602\u2013616. https://doi.org/10.1110/ps.041205428",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15689503",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "WT(F653W)",
      "MUTATION_PDB": "WT(F45W)",
      "T": 25.0,
      "pH": 5.0,
      "BUFFER_NAME": "Acetate",
      "BUFFER_CONC": "0.02",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 7.33,
      "ln(ku)_H2O": -6.84,
      "dGKIN_H2O": 35.11,
      "mf": -5.66,
      "mu": 3.43,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Maxwell, K.L., Wildes, D., Zarrine-Afsar, A., De Los Rios, M.A., Brown, A.G., Friel, C.T., Hedberg, L., Horng, J.-C., Bona, D., Miller, E.J., Vall\u00e9e-B\u00e9lisle, A., Main, E.R.G., Bemporad, F., Qiu, L., Teilum, K., Vu, N.-D., Edwards, A.M., Ruczinski, I., Poulsen, F.M., Kragelund, B.B., Michnick, S.W., Chiti, F., Bai, Y., Hagen, S.J., Serrano, L., Oliveberg, M., Raleigh, D.P., Wittung-Stafshede, P., Radford, S.E., Jackson, S.E., Sosnick, T.R., Marqusee, S., Davidson, A.R., Plaxco, K.W.",
      "REFERENCE_KIN": "Protein folding: defining a \u201cstandard\u201d set of experimental conditions and a preliminary kinetic data set of two-state proteins. Protein Sci. 14, 602\u2013616. https://doi.org/10.1110/ps.041205428",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15689503",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "WT(F653W)",
      "MUTATION_PDB": "WT(F45W)",
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      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.85,
      "ln(ku)_H2O": -6.43,
      "dGKIN_H2O": 30.42,
      "mf": -6.57,
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      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi025",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
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      "MUTATION_PDB": "WT(F45W)",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      "ln(ku)_H2O": -6.43,
      "dGKIN_H2O": 30.42,
      "mf": -6.57,
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      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi025",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
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         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I611V",
      "MUTATION_PDB": "I3V",
      "SEC_STR": "Beta",
      "RSA": "0.0",
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      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.06,
      "dln(kf)_H2O": -0.34,
      "ln(ku)_H2O": -4.57,
      "dln(ku)_H2O": 4.59,
      "dGKIN_H2O": 23.86,
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      "PhiF_H2O": 0.4,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi026",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I611V",
      "MUTATION_PDB": "I3V",
      "SEC_STR": "Beta",
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi026",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "I611A",
      "MUTATION_PDB": "I3A",
      "SEC_STR": "Beta",
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      "BUFFER_NAME": "Tris-HCl",
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      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 3.78,
      "dln(kf)_H2O": -2.07,
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi027",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "I611A",
      "MUTATION_PDB": "I3A",
      "SEC_STR": "Beta",
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      "BUFFER_NAME": "Tris-HCl",
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      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 3.78,
      "dln(kf)_H2O": -2.07,
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi027",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "V613A",
      "MUTATION_PDB": "V5A",
      "SEC_STR": "Beta",
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      "BUFFER_NAME": "Tris-HCl",
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      "METHOD_KIN": "GuHCl",
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      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi028",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "V613A",
      "MUTATION_PDB": "V5A",
      "SEC_STR": "Beta",
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      "METHOD_KIN": "GuHCl",
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         "F653W"
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi028",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "T615A",
      "MUTATION_PDB": "T7A",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi029",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_PDB": "T7A",
      "SEC_STR": "Beta",
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         "F653W"
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi029",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
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         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "T617A",
      "MUTATION_PDB": "T9A",
      "SEC_STR": "Coil",
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      "BUFFER_CONC": "0.05",
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         "F653W"
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      "BACKGROUND_MUT_PDB": [
         "F45W"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi030",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "T617A",
      "MUTATION_PDB": "T9A",
      "SEC_STR": "Coil",
      "RSA": "87.3",
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      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.69,
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      "dGKIN_H2O": 24.75,
      "ddGKIN_H2O": -5.67,
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi030",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I621V",
      "MUTATION_PDB": "I13V",
      "SEC_STR": "Beta",
      "RSA": "3.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.03,
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      "ln(ku)_H2O": -4.28,
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      "dGKIN_H2O": 23.07,
      "ddGKIN_H2O": -7.35,
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      "BACKGROUND_MUT_UNIPROT": [
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi031",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I621V",
      "MUTATION_PDB": "I13V",
      "SEC_STR": "Beta",
      "RSA": "3.0",
      "T": 25.0,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      "BACKGROUND_MUT_UNIPROT": [
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi031",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I621A",
      "MUTATION_PDB": "I13A",
      "SEC_STR": "Beta",
      "RSA": "3.0",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 3.4,
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi032",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
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      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I621A",
      "MUTATION_PDB": "I13A",
      "SEC_STR": "Beta",
      "RSA": "3.0",
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      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 3.4,
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi032",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
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      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "L623A",
      "MUTATION_PDB": "L15A",
      "SEC_STR": "Beta",
      "RSA": "4.3",
      "T": 25.0,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi033",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_PDB": "L15A",
      "SEC_STR": "Beta",
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi033",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_PDB": "V17A",
      "SEC_STR": "Coil",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi034",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
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      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "V625A",
      "MUTATION_PDB": "V17A",
      "SEC_STR": "Coil",
      "RSA": "2.8",
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      "BUFFER_CONC": "0.05",
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      "METHOD_KIN": "GuHCl",
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi034",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "D629N",
      "MUTATION_PDB": "D21N",
      "SEC_STR": "Coil",
      "RSA": "22.7",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi035",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "D629N",
      "MUTATION_PDB": "D21N",
      "SEC_STR": "Coil",
      "RSA": "22.7",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi035",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "T630A",
      "MUTATION_PDB": "T22A",
      "SEC_STR": "Beta",
      "RSA": "33.1",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi036",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "T630A",
      "MUTATION_PDB": "T22A",
      "SEC_STR": "Beta",
      "RSA": "33.1",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi036",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
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         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631V",
      "MUTATION_PDB": "I23V",
      "SEC_STR": "Helix",
      "RSA": "0.0",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      "BACKGROUND_MUT_UNIPROT": [
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi037",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631V",
      "MUTATION_PDB": "I23V",
      "SEC_STR": "Helix",
      "RSA": "0.0",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      "ln(ku)_H2O": -5.54,
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      "dGKIN_H2O": 26.56,
      "ddGKIN_H2O": -3.86,
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      "BACKGROUND_MUT_UNIPROT": [
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi037",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
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      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631A",
      "MUTATION_PDB": "I23A",
      "SEC_STR": "Helix",
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi038",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631A",
      "MUTATION_PDB": "I23A",
      "SEC_STR": "Helix",
      "RSA": "0.0",
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      "BUFFER_CONC": "0.05",
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      ],
      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi038",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631G",
      "MUTATION_PDB": "I23G",
      "SEC_STR": "Helix",
      "RSA": "0.0",
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi039",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I631G",
      "MUTATION_PDB": "I23G",
      "SEC_STR": "Helix",
      "RSA": "0.0",
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      "MEASURE_KIN": "stopped-flow",
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi039",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "V634A",
      "MUTATION_PDB": "V26A",
      "SEC_STR": "Helix",
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi040",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
      "PFAM": [
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         "1ubqA00 (3.10.20.90)"
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      "MUTATION_UNIPROT": "V634A",
      "MUTATION_PDB": "V26A",
      "SEC_STR": "Helix",
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      "BUFFER_CONC": "0.05",
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      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi040",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
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      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
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      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "K635A",
      "MUTATION_PDB": "K27A",
      "SEC_STR": "Helix",
      "RSA": "6.3",
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      "BUFFER_CONC": "0.05",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi041",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "K635A",
      "MUTATION_PDB": "K27A",
      "SEC_STR": "Helix",
      "RSA": "6.3",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
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      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi041",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "A636G",
      "MUTATION_PDB": "A28G",
      "SEC_STR": "Helix",
      "RSA": "41.5",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
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      "BACKGROUND_MUT_PDB": [
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi042",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
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      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "A636G",
      "MUTATION_PDB": "A28G",
      "SEC_STR": "Helix",
      "RSA": "41.5",
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      "pH": 7.4,
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      "BUFFER_CONC": "0.05",
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi042",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
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      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I638V",
      "MUTATION_PDB": "I30V",
      "SEC_STR": "Helix",
      "RSA": "0.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
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      ],
      "BACKGROUND_MUT_PDB": [
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      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi043",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
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      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi043",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
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         "1ubqA00 (3.10.20.90)"
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      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi044",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi044",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "PMID_KIN": "15857839",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi048",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi049",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi049",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "mf": -5.48,
      "mu": 3.25,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi050",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "I669A",
      "MUTATION_PDB": "I61A",
      "SEC_STR": "Coil",
      "RSA": "3.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.23,
      "dln(kf)_H2O": -0.62,
      "ln(ku)_H2O": -0.6,
      "dln(ku)_H2O": 8.56,
      "dGKIN_H2O": 14.44,
      "ddGKIN_H2O": -15.98,
      "mf": -5.48,
      "mu": 3.25,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi050",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "L675A",
      "MUTATION_PDB": "L67A",
      "SEC_STR": "Beta",
      "RSA": "0.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.45,
      "dln(kf)_H2O": -0.4,
      "ln(ku)_H2O": -1.74,
      "dln(ku)_H2O": 7.42,
      "dGKIN_H2O": 17.81,
      "ddGKIN_H2O": -12.61,
      "mf": -7.09,
      "mu": 3.27,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi051",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "L675A",
      "MUTATION_PDB": "L67A",
      "SEC_STR": "Beta",
      "RSA": "0.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.45,
      "dln(kf)_H2O": -0.4,
      "ln(ku)_H2O": -1.74,
      "dln(ku)_H2O": 7.42,
      "dGKIN_H2O": 17.81,
      "ddGKIN_H2O": -12.61,
      "mf": -7.09,
      "mu": 3.27,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi051",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "L677A",
      "MUTATION_PDB": "L69A",
      "SEC_STR": "Beta",
      "RSA": "3.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.32,
      "dln(kf)_H2O": -0.53,
      "ln(ku)_H2O": -1.3,
      "dln(ku)_H2O": 7.86,
      "dGKIN_H2O": 16.4,
      "ddGKIN_H2O": -14.02,
      "mf": -7.38,
      "mu": 2.35,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi052",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "Ubiquitin",
      "SOURCE": "Homo sapiens",
      "LENGTH": 76.0,
      "UniProt": "P0CG48",
      "PDB_wild": "1ubq",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF00240"
      ],
      "CATH": [
         "1ubqA00 (3.10.20.90)"
      ],
      "MUTATION_UNIPROT": "L677A",
      "MUTATION_PDB": "L69A",
      "SEC_STR": "Beta",
      "RSA": "3.0",
      "T": 25.0,
      "pH": 7.4,
      "BUFFER_NAME": "Tris-HCl",
      "BUFFER_CONC": "0.05",
      "MEASURE_KIN": "stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 5.32,
      "dln(kf)_H2O": -0.53,
      "ln(ku)_H2O": -1.3,
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      "dGKIN_H2O": 16.4,
      "ddGKIN_H2O": -14.02,
      "mf": -7.38,
      "mu": 2.35,
      "PhiF_H2O": 0.1,
      "BACKGROUND_MUT_UNIPROT": [
         "F653W"
      ],
      "BACKGROUND_MUT_PDB": [
         "F45W"
      ],
      "AUTHOR_KIN": "Went, H.M., Jackson, S.E.",
      "REFERENCE_KIN": "Ubiquitin folds through a highly polarized transition state. Protein Eng. Des. Sel. 18, 229\u2013237. https://doi.org/10.1093/protein/gzi052",
      "YEAR_KIN": 2005.0,
      "PMID_KIN": "15857839",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   }
]