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      "YEAR_KIN": 2006.0,
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "PMID_KIN": "16406408",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.026",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
      "STATE": "2",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.027",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.027",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.030",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
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      "REVIEW_DATE": "2023-06-04T01:00:00"
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      "PMID_KIN": "16406408",
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      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "E3BD (F166W)",
      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
      "LENGTH": 45.0,
      "UniProt": "P11961",
      "PDB_wild": "1w4e",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF02817"
      ],
      "CATH": [
         "1w4eA00 (4.10.320.10)"
      ],
      "EC_NUMBER": "2.3.1.12",
      "MUTATION_UNIPROT": "V135A",
      "MUTATION_PDB": "V135A",
      "SEC_STR": "Helix",
      "RSA": "2.1",
      "T": 25.0,
      "pH": 5.5,
      "BUFFER_NAME": "Sodium Acetate",
      "BUFFER_CONC": "0.02",
      "MEASURE_KIN": "T-jump or stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 9.93,
      "dln(kf)_H2O": -0.3,
      "ln(ku)_H2O": 6.51,
      "dln(ku)_H2O": 3.51,
      "mf": -1.92,
      "mu": 0.71,
      "BACKGROUND_MUT_UNIPROT": [
         "F166W"
      ],
      "AUTHOR_KIN": "Ferguson, N.,  Sharpe, T.D., Johnson, C.M., Fersht, A.R.",
      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.020",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "E3BD (F166W)",
      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
      "LENGTH": 45.0,
      "UniProt": "P11961",
      "PDB_wild": "1w4e",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF02817"
      ],
      "CATH": [
         "1w4eA00 (4.10.320.10)"
      ],
      "EC_NUMBER": "2.3.1.12",
      "MUTATION_UNIPROT": "Y138F",
      "MUTATION_PDB": "Y138F",
      "SEC_STR": "Helix",
      "RSA": "18.9",
      "T": 25.0,
      "pH": 5.5,
      "BUFFER_NAME": "Sodium Acetate",
      "BUFFER_CONC": "0.02",
      "MEASURE_KIN": "T-jump or stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 9.45,
      "dln(kf)_H2O": -0.78,
      "ln(ku)_H2O": 6.15,
      "dln(ku)_H2O": 3.15,
      "mf": -1.92,
      "mu": 1.05,
      "BACKGROUND_MUT_UNIPROT": [
         "F166W"
      ],
      "AUTHOR_KIN": "Ferguson, N.,  Sharpe, T.D., Johnson, C.M., Fersht, A.R.",
      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.021",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   },
   {
      "PROTEIN": "E3BD (F166W)",
      "SOURCE": "Geobacillus stearothermophilus (Bacillus stearothermophilus)",
      "LENGTH": 45.0,
      "UniProt": "P11961",
      "PDB_wild": "1w4e",
      "MUTATED_CHAIN": "A",
      "PFAM": [
         "PF02817"
      ],
      "CATH": [
         "1w4eA00 (4.10.320.10)"
      ],
      "EC_NUMBER": "2.3.1.12",
      "MUTATION_UNIPROT": "Y138F",
      "MUTATION_PDB": "Y138F",
      "SEC_STR": "Helix",
      "RSA": "18.9",
      "T": 25.0,
      "pH": 5.5,
      "BUFFER_NAME": "Sodium Acetate",
      "BUFFER_CONC": "0.02",
      "MEASURE_KIN": "T-jump or stopped-flow",
      "METHOD_KIN": "GuHCl",
      "ln(kf)_H2O": 9.45,
      "dln(kf)_H2O": -0.78,
      "ln(ku)_H2O": 6.15,
      "dln(ku)_H2O": 3.15,
      "mf": -1.92,
      "mu": 1.05,
      "BACKGROUND_MUT_UNIPROT": [
         "F166W"
      ],
      "AUTHOR_KIN": "Ferguson, N.,  Sharpe, T.D., Johnson, C.M., Fersht, A.R.",
      "REFERENCE_KIN": "The transition state for folding of a peripheral subunit-binding domain contains robust and ionic-strength dependent characteristics [WWW Document]. Journal of molecular biology. https://doi.org/10.1016/j.jmb.2005.12.021",
      "YEAR_KIN": 2006.0,
      "PMID_KIN": "16406408",
      "STATE": "2",
      "REVERSIBILITY": "yes",
      "REVIEW_DATE": "2023-06-04T01:00:00"
   }
]