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Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.104", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E127G", "MUTATION_PDB": "E161G", "SEC_STR": "Helix", "RSA": "58.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.14, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 0.51, "dGKIN_H2O": 10.62, "ddGKIN_H2O": -4.02, "mf": -2.89, "mu": 0.5, "PhiF_H2O": 0.9, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.106", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "E127G", "MUTATION_PDB": "E161G", "SEC_STR": "Helix", "RSA": "58.2", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 11.14, "dln(kf)_H2O": -1.11, "ln(ku)_H2O": 6.86, "dln(ku)_H2O": 0.51, "dGKIN_H2O": 10.62, "ddGKIN_H2O": -4.02, "mf": -2.89, "mu": 0.5, "PhiF_H2O": 0.9, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.106", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D128N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "16.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -2.61, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -9.95, "mf": -2.64, "mu": 0.67, "PhiF_H2O": 0.65, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.108", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" }, { "PROTEIN": "POB (L146A/Y166W)", "SOURCE": "Pyrobaculum aerophilum", "LENGTH": 51.0, "UniProt": "Q8ZUR6", "PDB_wild": "1w4j", "MUTATED_CHAIN": "A", "PFAM": [ "PF02817" ], "CATH": [ "1w4jA00 (4.10.320.10)" ], "EC_NUMBER": "2.3.1.12", "MUTATION_UNIPROT": "D128N", "MUTATION_PDB": "D162N", "SEC_STR": "Helix", "RSA": "16.6", "T": 25.0, "pH": 5.5, "BUFFER_NAME": "Sodium Acetate", "BUFFER_CONC": "0.05", "MEASURE_KIN": "T-jump or continuous-flow", "METHOD_KIN": "thermal or GuHCl", "ln(kf)_H2O": 9.64, "dln(kf)_H2O": -2.61, "ln(ku)_H2O": 7.74, "dln(ku)_H2O": 1.39, "dGKIN_H2O": 4.69, "ddGKIN_H2O": -9.95, "mf": -2.64, "mu": 0.67, "PhiF_H2O": 0.65, "BACKGROUND_MUT_UNIPROT": [ "L112A", "Y132W" ], "AUTHOR_KIN": "Sharpe, T.D., Ferguson, N., Johnson, C.M., Fersht, A.R.", "REFERENCE_KIN": "Conservation of Transition State Structure in Fast Folding Peripheral Subunit-Binding Domains. Journal of Molecular Biology 383, 224\u2013237. https://doi.org/10.1016/j.jmb.2008.06.108", "YEAR_KIN": 2008.0, "PMID_KIN": "18625240", "STATE": "2", "REVERSIBILITY": "yes", "REVIEW_DATE": "2023-06-04T01:00:00" } ]