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hydrogen bonds; tyrosine;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "Y81F", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1WQR, 1TCY", "MUTATED_CHAIN": "A", "MUTATION": "Y63F", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "25.5", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.24, "dCp": 1.53, "REVERSIBILITY": "yes", "AUTHOR": "Yamagata Y, Kubota M, Sumikawa Y, Funahashi J, Takano K,", "REFERENCE": "BIOCHEMISTRY 37, 9355-9362 (1998).", "PMID": "9649316", "KEY_WORDS": "conformational stability; hydrogen bonds; tyrosine;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "Y142F", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1WQM", "MUTATED_CHAIN": "A", "MUTATION": "Y124F", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "4.2", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.36, "dCp": 1.46, "REVERSIBILITY": "yes", "AUTHOR": "Yamagata Y, Kubota M, Sumikawa Y, Funahashi J, Takano K,", "REFERENCE": "BIOCHEMISTRY 37, 9355-9362 (1998).", "PMID": "9649316", "KEY_WORDS": "conformational stability; hydrogen bonds; tyrosine;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "dHvH": 120.1, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; protein stability; cavity;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2HEB", "MUTATED_CHAIN": "A", "MUTATION": "I23A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -2.54, "dHvH": 115.5, "dCp": 1.2, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; protein stability; cavity;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2HEC", "MUTATED_CHAIN": "A", "MUTATION": "I56A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -3.71, "dHvH": 110.8, "dCp": 1.34, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; protein stability; cavity;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2HED", "MUTATED_CHAIN": "A", "MUTATION": "I59A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -1.72, "dHvH": 120.9, "dCp": 1.46, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; protein stability; cavity;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I107A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2HEF", "MUTATED_CHAIN": "A", "MUTATION": "I89A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "2.2", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -2.7, "dHvH": 113.0, "dCp": 1.65, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; protein stability; cavity;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I124A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2HEA", "MUTATED_CHAIN": "A", "MUTATION": "I106A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.3", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.3 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.93, "dHvH": 112.2, "dCp": 1.34, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Funahashi J, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "J MOL BIOL 274, 132-142 (1997).", "PMID": "9398521", "KEY_WORDS": "water molecule; 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surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF9", "MUTATED_CHAIN": "A", "MUTATION": "V2Y", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.21, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.0, "dHvH": 119.98, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF9", "MUTATED_CHAIN": "A", "MUTATION": "V2Y", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.24, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 73.0, "dHvH": 122.13, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.66, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 59.6, "dHvH": 99.9, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.96, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dHvH": 108.03, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.01, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 65.7, "dHvH": 108.99, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.14, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 68.0, "dHvH": 113.05, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "44.4", "pH": 3.3, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.2, "dHvH": 118.07, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.6, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 59.1, "dHvH": 99.9, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.82, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.7, "dHvH": 104.92, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.91, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.3, "dHvH": 108.99, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.01, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.3, "dHvH": 110.9, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.6, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.1, "dHvH": 109.94, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.88, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.4, "dHvH": 114.96, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.99, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 68.6, "dHvH": 119.98, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "44.4", "pH": 3.15, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.7, "dHvH": 123.09, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 3.3, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 74.1, "dHvH": 125.96, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "V74S", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.61, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.1, "dHvH": 109.94, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "V74S", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.83, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.0, "dHvH": 114.96, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "V74S", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.28, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.2, "dHvH": 121.89, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.67, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.3, "dHvH": 103.97, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.82, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.9, "dHvH": 111.14, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.02, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.0, "dHvH": 116.16, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.21, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 73.3, "dHvH": 119.98, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.26, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 74.1, "dHvH": 123.09, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.6, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 61.7, "dHvH": 109.94, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.83, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 65.9, "dHvH": 114.96, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.98, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 68.1, "dHvH": 118.07, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.1, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.1, "dHvH": 123.09, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.25, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.5, "dHvH": 126.91, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFK", "MUTATED_CHAIN": "A", "MUTATION": "V74N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.65, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.0, "dHvH": 114.01, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFK", "MUTATED_CHAIN": "A", "MUTATION": "V74N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.84, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.2, "dHvH": 115.92, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFK", "MUTATED_CHAIN": "A", "MUTATION": "V74N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.18, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 71.5, "dHvH": 123.09, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.66, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.8, "dHvH": 100.14, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.85, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 67.3, "dHvH": 105.16, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.0, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 69.5, "dHvH": 108.99, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.18, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.8, "dHvH": 114.01, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 3.22, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 73.2, "dHvH": 116.16, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.66, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.3, "dHvH": 105.16, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.85, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 67.3, "dHvH": 111.14, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.98, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 69.3, "dHvH": 114.01, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.18, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.2, "dHvH": 121.18, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.38, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 75.4, "dHvH": 125.0, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.63, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.9, "dHvH": 108.03, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.84, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 67.9, "dHvH": 112.09, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.0, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.4, "dHvH": 119.98, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.13, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.4, "dHvH": 122.13, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.34, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 75.5, "dHvH": 125.0, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.64, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.0, "dHvH": 108.99, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.83, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 67.2, "dHvH": 114.96, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.04, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.6, "dHvH": 118.07, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.13, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 71.7, "dHvH": 121.89, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.72, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 68.2, "dHvH": 108.99, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.8, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 69.3, "dHvH": 110.18, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.01, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 72.8, "dHvH": 116.16, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 3.15, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 75.0, "dHvH": 119.98, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dCp": 1.58, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF8", "MUTATED_CHAIN": "A", "MUTATION": "V2S", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.5, "dTm": -4.4, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF9", "MUTATED_CHAIN": "A", "MUTATION": "V2Y", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.7, "dTm": -1.2, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.4, "dTm": -4.5, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.8, "dTm": -4.1, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.6, "dTm": -1.3, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF8", "MUTATED_CHAIN": "A", "MUTATION": "V2S", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -1.41, "dCp": 1.55, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GF9", "MUTATED_CHAIN": "A", "MUTATION": "V2Y", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.36, "dCp": 1.82, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFA", "MUTATED_CHAIN": "A", "MUTATION": "V2D", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -1.43, "dCp": 1.58, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFE", "MUTATED_CHAIN": "A", "MUTATION": "V2N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "44.4", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -1.34, "dCp": 1.7, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFG", "MUTATED_CHAIN": "A", "MUTATION": "V2R", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.38, "dCp": 1.41, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "V74S", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.38, "dCp": 1.22, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.24, "dCp": 1.65, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.43, "dCp": 1.58, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFK", "MUTATED_CHAIN": "A", "MUTATION": "V74N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.33, "dCp": 1.1, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.07, "dCp": 1.67, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -0.14, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": 0.17, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": 0.07, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "T": 64.9, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": 0.88, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "V74S", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dCp": 1.22, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFH", "MUTATED_CHAIN": "A", "MUTATION": "V74Y", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.0, "dTm": -0.9, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFJ", "MUTATED_CHAIN": "A", "MUTATION": "V74D", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.5, "dTm": -1.4, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFK", "MUTATED_CHAIN": "A", "MUTATION": "V74N", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.9, "dTm": -1.0, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFR", "MUTATED_CHAIN": "A", "MUTATION": "V74R", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.6, "dTm": -0.3, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128Y", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFT", "MUTATED_CHAIN": "A", "MUTATION": "V110Y", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.4, "dTm": -0.5, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128D", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFU", "MUTATED_CHAIN": "A", "MUTATION": "V110D", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 65.4, "dTm": 0.5, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128N", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1GFV", "MUTATED_CHAIN": "A", "MUTATION": "V110N", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 65.1, "dTm": 0.2, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128R", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1INU", "MUTATED_CHAIN": "A", "MUTATION": "V110R", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 67.8, "dTm": 2.9, "REVERSIBILITY": "Unknown", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K.", "REFERENCE": "J BIOL CHEM 277, 21792-21800 (2002).", "PMID": "11927576", "KEY_WORDS": "hydration structure; surface; alpha-helix; beta-sheet;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "T": 36.7, "pH": 2.35, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.5 - 1.0 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "dHvH": 58.08, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K.", "REFERENCE": "PROTEIN ENG 16, 5-9 (2003).", "PMID": "12646687", "KEY_WORDS": "cavity; human lysozyme; mutant protein; protein stability; water molecule", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "T": 47.2, "pH": 2.9, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.5 - 1.0 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "dHvH": 74.81, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K.", "REFERENCE": "PROTEIN ENG 16, 5-9 (2003).", "PMID": "12646687", "KEY_WORDS": "cavity; human lysozyme; mutant protein; protein stability; water molecule", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.5 - 1.0 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 43.4, "dTm": -5.8, "dCp": 1.63, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K.", "REFERENCE": "PROTEIN ENG 16, 5-9 (2003).", "PMID": "12646687", "KEY_WORDS": "cavity; human lysozyme; mutant protein; protein stability; water molecule", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "T": 49.2, "pH": 2.7, "BUFFER_NAME": "Gly-HCl", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.5 - 1.0 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "ddG": -1.31, "dCp": 1.63, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K.", "REFERENCE": "PROTEIN ENG 16, 5-9 (2003).", "PMID": "12646687", "KEY_WORDS": "cavity; human lysozyme; mutant protein; protein stability; water molecule", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "P89G,P121G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "P71G,P103G", "NO_MOLECULE": "1", "SEC_STR": "Coil,Turn", "RSA": "56.4,100.0", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 8.6, "ddG_H2O": -1.1, "m": 5.48, "Cm": 2.64, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 9.7, "m": 5.52, "Cm": 2.97, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "P89G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHI", "MUTATED_CHAIN": "A", "MUTATION": "P71G", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "35.2", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 8.5, "ddG_H2O": -1.2, "m": 5.43, "Cm": 2.63, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "P121G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHJ", "MUTATED_CHAIN": "A", "MUTATION": "P103G", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "70.0", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 9.7, "m": 5.55, "Cm": 2.95, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "A65P", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHL", "MUTATED_CHAIN": "A", "MUTATION": "A47P", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "65.9", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 10.8, "ddG_H2O": 1.1, "m": 5.5, "Cm": 3.3, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "D109P", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHK", "MUTATED_CHAIN": "A", "MUTATION": "D91P", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "25.5", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 8.9, "ddG_H2O": -0.8, "m": 5.42, "Cm": 2.78, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128P", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHH", "MUTATED_CHAIN": "A", "MUTATION": "V110P", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 12.1, "ddG_H2O": 2.4, "m": 6.69, "Cm": 3.05, "REVERSIBILITY": "yes (100%)", "AUTHOR": "Herning T., Yutani K., Taniyama Y. & Kikuchi M.", "REFERENCE": "BIOCHEMISTRY 30, 9882-9891 (1991).", "PMID": "1911779", "KEY_WORDS": "human lysozyme; refolding kinetics; proline mutations;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 49.2, "dCp": 1.67, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQC", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 47.6, "dTm": -1.6, "dCp": 1.82, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQD", "MUTATED_CHAIN": "A", "MUTATION": "I56V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 42.9, "dTm": -6.3, "dCp": 1.29, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQE", "MUTATED_CHAIN": "A", "MUTATION": "I59V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 44.8, "dTm": -4.4, "dCp": 1.67, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I107V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQF", "MUTATED_CHAIN": "A", "MUTATION": "I89V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "2.2", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 46.5, "dTm": -2.7, "dCp": 1.84, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I124V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQB", "MUTATED_CHAIN": "A", "MUTATION": "I106V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.3", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 44.5, "dTm": -4.7, "dCp": 1.72, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUG", "MUTATED_CHAIN": "A", "MUTATION": "V2A", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 41.3, "dTm": -7.9, "dCp": 1.44, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQM", "MUTATED_CHAIN": "A", "MUTATION": "V74A", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 47.3, "dTm": -1.9, "dCp": 1.53, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V111A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQN", "MUTATED_CHAIN": "A", "MUTATION": "V93A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 44.8, "dTm": -4.4, "dCp": 1.53, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; 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hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQH", "MUTATED_CHAIN": "A", "MUTATION": "V110A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 49.5, "dTm": 0.3, "dCp": 1.51, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V139A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQI", "MUTATED_CHAIN": "A", "MUTATION": "V121A", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "6.0", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 39.9, "dTm": -9.3, "dCp": 1.37, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V143A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQJ", "MUTATED_CHAIN": "A", "MUTATION": "V125A", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "12.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 42.2, "dTm": -7.0, "dCp": 1.56, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V148A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "2BQK", "MUTATED_CHAIN": "A", "MUTATION": "V130A", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "38.9", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 44.4, "dTm": -4.8, "dCp": 1.87, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; double mutant;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 3.14, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 57.8, "dHvH": 97.4, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 3.04, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 55.4, "dHvH": 95.0, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.81, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 52.0, "dHvH": 89.2, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 49.2, "dHvH": 85.4, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.53, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 45.5, "dHvH": 79.8, "REVERSIBILITY": "yes", "REMARKS": "pseudo wild type human lysozyme, 1LHM (C77A, C95A)", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 3.09, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 55.0, "dHvH": 95.0, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.96, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 52.3, "dHvH": 89.2, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.78, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 49.6, "dHvH": 85.9, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.66, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 46.7, "dHvH": 80.4, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; hydrophobic effect; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.49, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 43.5, "dHvH": 74.6, "REVERSIBILITY": "yes", "REMARKS": "ddG and dTm are relative to pseudo wild type", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "J MOL BIOL 280, 749-761 (1998).", "PMID": "9677301", "KEY_WORDS": "protein stability; 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human lysozyme; mutant protein; protein stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77F", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59F", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K", "REFERENCE": "PROTEIN ENG 12, 841-850 (1999).", "PMID": "10556244", "KEY_WORDS": "calorimetry; human lysozyme; mutant protein; protein stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I77S", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "I59S", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.0", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Yamagata Y, Yutani K", "REFERENCE": "PROTEIN ENG 12, 841-850 (1999).", "PMID": "10556244", "KEY_WORDS": "calorimetry; 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human lysozyme; mutant protein; protein stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dHvH": 120.1, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Ogasahara K, Kaneda H, Yamagata Y, Fujii S, Kanaya E,", "REFERENCE": "J MOL BIOL 254, 62-76 (1995).", "PMID": "7473760", "KEY_WORDS": "protein stability; hydrophobicity; cavity; calorimetry; human lysozyme", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I41V", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1YAN", "MUTATED_CHAIN": "A", "MUTATION": "I23V", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "4.8", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.8, "dTm": -1.1, "dHvH": 120.3, "dCp": 1.39, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Ogasahara K, Kaneda H, Yamagata Y, Fujii S, Kanaya E,", "REFERENCE": "J MOL BIOL 254, 62-76 (1995).", "PMID": "7473760", "KEY_WORDS": "protein stability; 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hydrophobicity; cavity; calorimetry; human lysozyme", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 20.0, "pH": 5.2, "BUFFER_NAME": "Sodium acetate", "BUFFER_CONC": "20 mM", "PROTEIN_CONC": "2-13 micro M", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 14.7, "m": 3.6, "Cm": 4.12, "REVERSIBILITY": "yes", "AUTHOR": "Arai M, Hamel P, Kanaya E, Inaka K, Miki K, Kikuchi M, Kuwajima K", "REFERENCE": "BIOCHEMISTRY 39, 3472-3479 (2000).", "PMID": "10727242", "KEY_WORDS": "disulfide bonds; stability; folding; free-energy", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "W82C,C83A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "W64C,C65A", "NO_MOLECULE": "1", "SEC_STR": "Turn,Sheet", "RSA": "13.5,0.0", "T": 20.0, "pH": 5.2, "BUFFER_NAME": "Sodium acetate", "BUFFER_CONC": "20 mM", "PROTEIN_CONC": "2-13 micro M", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 12.5, "ddG_H2O": -2.2, "m": 3.3, "Cm": 3.79, "REVERSIBILITY": "yes", "AUTHOR": "Arai M, Hamel P, Kanaya E, Inaka K, Miki K, Kikuchi M, Kuwajima K", "REFERENCE": "BIOCHEMISTRY 39, 3472-3479 (2000).", "PMID": "10727242", "KEY_WORDS": "disulfide bonds; stability; folding; free-energy", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.63, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 55.6, "dHvH": 96.32, "dCp": 1.94, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.64, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 56.4, "dHvH": 95.6, "dCp": 1.63, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.68, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 56.8, "dHvH": 96.32, "dCp": 1.94, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.75, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 58.2, "dHvH": 98.95, "dCp": 1.77, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.78, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 59.0, "dHvH": 101.1, "dCp": 1.31, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.84, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.3, "dHvH": 103.01, "dCp": 1.67, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.87, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.5, "dHvH": 98.95, "dCp": 1.7, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.93, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.5, "dHvH": 102.06, "dCp": 1.46, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 3.03, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.8, "dHvH": 102.06, "dCp": 1.79, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 9.49, "m": 3.23, "Cm": 2.9, "REVERSIBILITY": "yes", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 4.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 12.86, "m": 3.37, "Cm": 3.8, "REVERSIBILITY": "yes", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Sodium phosphate", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 12.05, "m": 3.01, "Cm": 4.0, "REVERSIBILITY": "yes", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 6.19, "m": 3.01, "Cm": 2.1, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 4.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 8.25, "m": 2.96, "Cm": 2.8, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 25.0, "pH": 7.0, "BUFFER_NAME": "Sodium phosphate", "BUFFER_CONC": "50 mM", "PROTEIN_CONC": "0.9-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "GdnHCl", "dG_H2O": 8.51, "m": 2.96, "Cm": 2.9, "REVERSIBILITY": "yes", "REMARKS": "four extra residues (Glu-4-Ala-3-Glu-2-Ala-1-) were added at", "AUTHOR": "Goda S, Takano K, Yamagata Y, Katakura Y, Yutani K.", "REFERENCE": "PROTEIN ENG 13, 299-307 (2000).", "PMID": "10810162", "KEY_WORDS": "crystal structure; extra N-terminal residues; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.8-0.9 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dHvH": 120.1, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Ogasahara K, Yamagata Y, Yutani K", "REFERENCE": "J BIOCHEM 120, 1216-1223 (1996).", "PMID": "9010773", "KEY_WORDS": "amyloid formation; denatured state of a protein; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74T", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUA, 1LOZ", "MUTATED_CHAIN": "A", "MUTATION": "I56T", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.8-0.9 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 52.4, "dTm": -12.5, "dHvH": 108.16, "dCp": 1.24, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Ogasahara K, Yamagata Y, Yutani K", "REFERENCE": "J BIOCHEM 120, 1216-1223 (1996).", "PMID": "9010773", "KEY_WORDS": "amyloid formation; denatured state of a protein; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74T", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUA, 1LOZ", "MUTATED_CHAIN": "A", "MUTATION": "I56T", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "pH": 3.11, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.8-0.9 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.52, "dHvH": 103.3, "dCp": 1.15, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Ogasahara K, Yamagata Y, Yutani K", "REFERENCE": "J BIOCHEM 120, 1216-1223 (1996).", "PMID": "9010773", "KEY_WORDS": "amyloid formation; denatured state of a protein; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74T", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUA, 1LOZ", "MUTATED_CHAIN": "A", "MUTATION": "I56T", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "pH": 2.98, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.8-0.9 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 57.96, "dHvH": 102.2, "dCp": 1.2, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Ogasahara K, Yamagata Y, Yutani K", "REFERENCE": "J BIOCHEM 120, 1216-1223 (1996).", "PMID": "9010773", "KEY_WORDS": "amyloid formation; denatured state of a protein; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "I74T", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUA, 1LOZ", "MUTATED_CHAIN": "A", "MUTATION": "I56T", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "0.3", "pH": 2.67, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.8-0.9 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 51.78, "dHvH": 90.9, "dCp": 1.0, "REVERSIBILITY": "yes", "AUTHOR": "Funahashi J, Takano K, Ogasahara K, Yamagata Y, Yutani K", "REFERENCE": "J BIOCHEM 120, 1216-1223 (1996).", "PMID": "9010773", "KEY_WORDS": "amyloid formation; denatured state of a protein; human lysozyme;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dHvH": 120.1, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V20A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUG", "MUTATED_CHAIN": "A", "MUTATION": "V2A", "NO_MOLECULE": "1", "SEC_STR": "Sheet", "RSA": "44.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.3, "dTm": -4.6, "dHvH": 114.2, "dCp": 1.56, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V92A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUH", "MUTATED_CHAIN": "A", "MUTATION": "V74A", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "39.9", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 63.8, "dTm": -1.1, "dHvH": 119.8, "dCp": 1.48, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V111A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUI", "MUTATED_CHAIN": "A", "MUTATION": "V93A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.6, "dTm": -2.3, "dHvH": 119.8, "dCp": 1.53, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V117A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUJ", "MUTATED_CHAIN": "A", "MUTATION": "V99A", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "1.8", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 61.9, "dTm": -3.0, "dHvH": 116.6, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V118A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUB", "MUTATED_CHAIN": "A", "MUTATION": "V100A", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "0.5", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.1, "dTm": -0.8, "dHvH": 119.9, "dCp": 1.0, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V128A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUC", "MUTATED_CHAIN": "A", "MUTATION": "V110A", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "35.7", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 66.4, "dTm": 1.5, "dHvH": 121.88, "dCp": 1.24, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V139A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUD", "MUTATED_CHAIN": "A", "MUTATION": "V121A", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "6.0", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.4, "dTm": -4.5, "dHvH": 112.3, "dCp": 1.53, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V143A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUE", "MUTATED_CHAIN": "A", "MUTATION": "V125A", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "12.4", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 60.9, "dTm": -4.0, "dHvH": 116.4, "dCp": 1.29, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "V148A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1OUF", "MUTATED_CHAIN": "A", "MUTATION": "V130A", "NO_MOLECULE": "1", "SEC_STR": "Coil", "RSA": "38.9", "pH": 2.7, "BUFFER_NAME": "glycine", "BUFFER_CONC": "0.05 M", "PROTEIN_CONC": "0.7-1.5 mg/ml", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 62.3, "dTm": -2.6, "dHvH": 119.4, "dCp": 1.51, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Fujii S, Yutani K", "REFERENCE": "BIOCHEMISTRY 36, 688-698 (1997).", "PMID": "9020766", "KEY_WORDS": "human lysozyme; calorimetry; hydrophobic effect; conformational stability;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 10.0, "pH": 4.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 14.0, "REVERSIBILITY": "yes", "AUTHOR": "Taniyama Y., Ogasahara K., Yutani K. & Kikuchi M.", "REFERENCE": "J BIOL CHEM 267, 4619-4624 (1992).", "PMID": "1537844", "KEY_WORDS": "human lysozyme; circular dichroism; disulfide bond; stabilization;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "C95A,C113A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHM", "MUTATED_CHAIN": "A", "MUTATION": "C77A,C95A", "NO_MOLECULE": "1", "SEC_STR": "Turn,Helix", "RSA": "15.3,1.3", "T": 10.0, "pH": 4.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "CD", "METHOD": "GdnHCl", "dG_H2O": 8.2, "ddG_H2O": -5.8, "REVERSIBILITY": "yes", "AUTHOR": "Taniyama Y., Ogasahara K., Yutani K. & Kikuchi M.", "REFERENCE": "J BIOL CHEM 267, 4619-4624 (1992).", "PMID": "1537844", "KEY_WORDS": "human lysozyme; circular dichroism; disulfide bond; stabilization;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "T": 10.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "Fluorescence", "METHOD": "GdnHCl", "dG_H2O": 11.8, "REVERSIBILITY": "yes", "AUTHOR": "Taniyama Y., Ogasahara K., Yutani K. & Kikuchi M.", "REFERENCE": "J BIOL CHEM 267, 4619-4624 (1992).", "PMID": "1537844", "KEY_WORDS": "human lysozyme; circular dichroism; disulfide bond; stabilization;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "C95A,C113A", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "PDB_mutant": "1LHM", "MUTATED_CHAIN": "A", "MUTATION": "C77A,C95A", "NO_MOLECULE": "1", "SEC_STR": "Turn,Helix", "RSA": "15.3,1.3", "T": 10.0, "pH": 3.0, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "40 mM", "MEASURE": "Fluorescence", "METHOD": "GdnHCl", "dG_H2O": 6.1, "ddG_H2O": -5.7, "REVERSIBILITY": "yes", "AUTHOR": "Taniyama Y., Ogasahara K., Yutani K. & Kikuchi M.", "REFERENCE": "J BIOL CHEM 267, 4619-4624 (1992).", "PMID": "1537844", "KEY_WORDS": "human lysozyme; circular dichroism; disulfide bond; stabilization;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; conformational stability; conformational entropy;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "R68G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "R50G", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "27.3", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 65.8, "dTm": 0.9, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; conformational stability; conformational entropy;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "Q76G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "Q58G", "NO_MOLECULE": "1", "SEC_STR": "Turn", "RSA": "2.3", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 70.6, "dTm": 5.7, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; conformational stability; conformational entropy;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "H96G", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "H78G", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "47.8", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.4, "dTm": -0.5, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; conformational stability; conformational entropy;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "G55Q", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "G37Q", "NO_MOLECULE": "1", "SEC_STR": "Helix", "RSA": "57.5", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.1, "dTm": -0.8, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; conformational stability; conformational entropy;", "REVIEW_DATE": "2020/09/13" }, { "PROTEIN": "Lysozyme", "SOURCE": "Homo sapiens (Human)", "LENGTH": "148", "UniProt": "P61626", "MUTATION_uni": "WT", "EC_NUMBER": "3.2.1.17", "PDB_wild": "1LZ1", "MUTATED_CHAIN": "A", "MUTATION": "WT", "NO_MOLECULE": "1", "pH": 2.7, "BUFFER_NAME": "glycine-HCl", "BUFFER_CONC": "0.05 M", "MEASURE": "DSC", "METHOD": "Thermal", "Tm": 64.9, "dCp": 1.58, "REVERSIBILITY": "yes", "AUTHOR": "Takano K, Yamagata Y, Yutani K", "REFERENCE": "BIOCHEMISTRY 39, 8655-8665 (2000).", "PMID": "10913274", "KEY_WORDS": "turn; 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